Ahi1 (NM_001002277) Rat Tagged ORF Clone

CAT#: RR215028

Ahi1 (Myc-DDK-tagged ORF) - Rat Abelson helper integration site 1 (Ahi1), (10 ug)

Product Images

Circular map for RR215028

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ahi1
• Synonyms: Ahi-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215028 representing NM_001002277
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAAGAGACTCCAGAGAAGGTGGACTCTGCACAGGAGAAGGTCAGAGGCAAGTCCCAGCCAGCAG
  ATGACTCGGAGGACAGCCGAGAAAAGGCTGGGACAGAGGGGACGGGAGAACTGACCGAAGCCTATGAGCT
  GCAGGTAGCTGAAGAAATGGCAAAAGAGATCAAGAAGAAAATAAGAAGGAAGCTAAAGGAGCAGCTGACC
  TACTTTCCTCCAGACACTCTGCTGCATGACGACAAGCTGGGCAGTGAAAAAAGGAAGAAGAAGAAGAAGA
  AAAAAGTCCCAGTGCCAGCTAAGCCTGAGACGAGTCCCTCAGATGTCTGTGACAGTGCAGCTGAAGGTGA
  GCAAAAGAAAGAAGGCGCCCCTGAGGGGTCTCACCACAGGGAGGGAGGATGCTCAACAGAACAGAATGCA
  GATGCTAGTGTACCAGAGAACACAAAGCCCAAACCCAAGAAGATGAAGAAGAAGCCTAAAGCAGTTTCAG
  AGGATAATGAAGAGACTAATGGAGATGGTGTTCATGAGATAACAGGCCGAGACAGCCCAGTTCATCCCAA
  GTGTTTGCTCGATGACGACCTCGTCATGGGAGTCTACATTCACCGAACCGATCGGCTTAAATCTGACTTT
  ATGATTTCTCACCCAATGGTAAAGATCCATGTGGTTGATGAACACACTGGCCAGTACGTCAAGAAAGATG
  ACAGTGAGCGTCCCGTCTCATCTTACTATGAGAAAGACAACGTGGACTATATTCTCCCTATTATGACGCA
  GCCGTACGACTTTAAAAAGCTAAAGTCAAGGCTTCCAGAGTGGGAGGAACAAGTTATTTTTAATGAAAAT
  TTTCCCTATTTGCTTCGAGAGTTTGATGAGTGCCCAAAAGTCATTCTGTTCTTTGAGATTCTTGACTTTT
  TAAGCATGGATGAAATCAAGAATAACTCTGAGTTTCAAAACCAAGAATGTGGCTTTCGGAAAATTGCCTG
  GGCATTTCTTAAGCTCCTGGGAGCCAATGGAAATGCAAACATCAACTCAAAACTTCGTCTGCAGCTCTAC
  TACCCACCTACTAAGCCTCGATCCCAGCCAAATGTTGTTGAGGTTTTTGAGTGGTGGTCCAAGTGTCCAA
  GGAATCGTTACCCATCAACACTGTATGTAACCGTACGAGGGTTGAAAGTCCCGGACTGCATAAAGCCATC
  GTACCGCTCTATGATGGCTCTCCAGGAGGAAAGGGGTACACCAGTGTACTGTGAACGCCACCGTGAAACA
  AGTTCTGTGGACACAGAACCTGGATTAGAAGATTCAAAGGAGGAAGTAAAGTGGAAACGTCTGCCGGGCC
  AGGCCTGTCGTATCCCAAACAAGCATCTCTTCTCACTAAATGCTGGAGAGCGAGGGTGTTTTTGCCTTGA
  TTTCTCCCACAATGGAAGGATATTAGCAGCAGCCTGTGCCAGCCGAGACGGATATCCGATTATACTATAC
  GAAATTCCTTCTGGACGTTTCATGAGAGAATTGTGTGGCCACCTCAATATCATTTATGATCTCGACTGGT
  CAAAAGACGATCGCTATCTTGTTACCTCATCATCTGATGGCACTGCCAGGGTTTGGAAGAATGAAATCAA
  CAGCACAAGCACGTTCAGAGTCTTACCTCACCCTTCCTTCGTCTACACGGCTAGATTCCACCCCGCCACA
  CGAGAGCTGGTGGTTACGGGATGCTATGACTCTATGATTCGGATATGGAAGGTGGATGCGAGGGAAGATG
  CCGCCATCTTAGTGCGCCAGTTGGATGTCCACAAGAGCTTCGTCAACTCCATCTGTTTTGATGATGAAGG
  TCACCACATGTACTCAGGAGACTGCATCGGCGTCATCGCTGTGTGGGACACTTATGTCAAAGTGACTGAT
  GTGCAGCACTCTGTGCGCCACTGGACAATAAACAAGGAAATTAAGGAAACTGAATTCAGGGGAGTTCCAG
  TCAGTTACCTGGAGGTGCATCCCAACGGAAAGCGTTTATTAATCCACACCAAAGACAGTACATTGAGGAT
  CATGGATCTGCGGATATTAGCAGCCAGGAAATTTGTGGGCGCAGCAAATTACCGGGAGAAGATCCACAGC
  ACTTTGACACCGTGTGGGACTCTGCTCTTTTCTGGCAGTGAGGATGGGATAGTGTACGTTTGGAACCCAG
  AGACAGGAGAACAAGTGGCAATGTATTCTGAGCTGCCGTTCAAGTCAACTATCCGAGACATCTCTTACCA
  TCCATTTGAGAACATGGTTGCGTTTTGTGCCTTTGGACAGAGCGAGCCCATTCTTCTTTACATTTATGAT
  TTCCAGGTGGCCCAGCAGGAGGCTGAAATGTTGAAGCGCTACAGTGGGACAGTCCCGTTACCTGGGATCC
  ATCTGAGTGAGGATGCTCTATGCACCTGCCCTAAACTGCCCCAGCAGGGCTCTTTCCAGATCGATGAATT
  TGTCAACACTGAAAATAATTCATCAAGAAAGATTCAGCTTGTGAAACAGAGACTCGAAACTGTCACAGAG
  GTGATACGATCCTGTGCTGCAAAAGTCAACAAAAATCTCTCAATCACTTCACCACCACCAGGCCCTGCAA
  AGAAGCCAAGGGTGAAACAGTCATTCGTGCTGACCACCGATCAGATCATACATCAGTTTGGAGTCCCTCA
  GACGGCATTTATCAGCATAGAAAGAAGACCTTTTATGCGTCCAGTGGATCCGCCACCAATGGTAGTGGCT
  CTTTATGACTACACAGCGAGCCGATCAGATGAACTAACCATCCATCGCGGAGACATTATCCGAGTGTTCT
  TCAAAGATAACGAAGACTGGTGGTACGGCAGCTTAGGAAAGGGGCAGGAAGGGTTCTTTCCAGCTAATCA
  TGTGGCCAGTGAGACACTGTACCGAGACTCGCCCCCAAAGGTAAAGGAGCGGTCCCCTCCTTTAACTCCC
  AAGGAGAAAGCTAAAATGGAAAAGCCTCCAGCTTCTCGAAAGTCACTCATTAAGGACAGGTTCCTGGATT
  CCAGACTGGGCTCGAAGCCTATGGGACATTCTGAAAAGGGCAGAGATCAAAACTTCGAGGAGCGAGGACA
  CAAATCGGATATGGAGATGAAGAAAAGTGAGCCAACGGTCCGCAAAGTCACCCTGATAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215028 representing NM_001002277
  Red=Cloning site Green=Tags(s)
  
  MEQETPEKVDSAQEKVRGKSQPADDSEDSREKAGTEGTGELTEAYELQVAEEMAKEIKKKIRRKLKEQLT
  YFPPDTLLHDDKLGSEKRKKKKKKKVPVPAKPETSPSDVCDSAAEGEQKKEGAPEGSHHREGGCSTEQNA
  DASVPENTKPKPKKMKKKPKAVSEDNEETNGDGVHEITGRDSPVHPKCLLDDDLVMGVYIHRTDRLKSDF
  MISHPMVKIHVVDEHTGQYVKKDDSERPVSSYYEKDNVDYILPIMTQPYDFKKLKSRLPEWEEQVIFNEN
  FPYLLREFDECPKVILFFEILDFLSMDEIKNNSEFQNQECGFRKIAWAFLKLLGANGNANINSKLRLQLY
  YPPTKPRSQPNVVEVFEWWSKCPRNRYPSTLYVTVRGLKVPDCIKPSYRSMMALQEERGTPVYCERHRET
  SSVDTEPGLEDSKEEVKWKRLPGQACRIPNKHLFSLNAGERGCFCLDFSHNGRILAAACASRDGYPIILY
  EIPSGRFMRELCGHLNIIYDLDWSKDDRYLVTSSSDGTARVWKNEINSTSTFRVLPHPSFVYTARFHPAT
  RELVVTGCYDSMIRIWKVDAREDAAILVRQLDVHKSFVNSICFDDEGHHMYSGDCIGVIAVWDTYVKVTD
  VQHSVRHWTINKEIKETEFRGVPVSYLEVHPNGKRLLIHTKDSTLRIMDLRILAARKFVGAANYREKIHS
  TLTPCGTLLFSGSEDGIVYVWNPETGEQVAMYSELPFKSTIRDISYHPFENMVAFCAFGQSEPILLYIYD
  FQVAQQEAEMLKRYSGTVPLPGIHLSEDALCTCPKLPQQGSFQIDEFVNTENNSSRKIQLVKQRLETVTE
  VIRSCAAKVNKNLSITSPPPGPAKKPRVKQSFVLTTDQIIHQFGVPQTAFISIERRPFMRPVDPPPMVVA
  LYDYTASRSDELTIHRGDIIRVFFKDNEDWWYGSLGKGQEGFFPANHVASETLYRDSPPKVKERSPPLTP
  KEKAKMEKPPASRKSLIKDRFLDSRLGSKPMGHSEKGRDQNFEERGHKSDMEMKKSEPTVRKVTLIE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002277
• ORF Size: 3141 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002277.1, NP_001002277.1
• RefSeq Size: 3144 bp
• RefSeq ORF: 3144 bp
• Locus ID: 308923
• UniProt ID: Q6DTM3
• Cytogenetics: 1p12
• MW: 119.7 kDa
• Gene Summary: mutations in the human homolog are associated with Joubert syndrome, an autosomal recessive disorder resulting in severe cognitive disability [RGD, Feb 2006]