Rcan1 (NM_153724) Rat Tagged ORF Clone

CAT#: RR214962

Rcan1 (Myc-DDK-tagged ORF) - Rat regulator of calcineurin 1 (Rcan1), (10 ug)

Product Images

Circular map for RR214962

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rcan1
• Synonyms: Dscr1; Mcip1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214962 representing NM_153724
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATTTTAGGGACTTTAACTACAATTTTAGCTCCCTGATTGCCTGTGTGGCAAACGGTGATGTCTTCA
  GCGAAAGTGAGACCAGGGCCAAATTTGAATCCCTCTTCAGGACGTATGACAAGGACATCACCTTCCAGTA
  TTTTAAGAGCTTCAAGCGTGTCCGAATAAACTTCAGCAACCCCCTGTCTGCGGCGGACGCCAGGCTGCAG
  CTGCATAAGACAGAGTTCCTGGGGAAGGAGATGAAACTGTACTTTGCCCAGACTTTACACATAGGAAGTT
  CACACCTGGCTCCGCCCAATCCAGACAAACAGTTTCTCATCTCTCCCCCTGCCTCTCCACCTGTTGGCTG
  GAAACAAGTAGAAGACGCTACCCCAGTCATAAACTACGATCTTTTATATGCTATCTCCAAGCTGGGACCA
  GGAGAGAAGTACGAGCTACACGCAGCGACAGACACCACGCCCAGCGTGGTGGTCCACGTGTGTGAGAGTG
  ACCAAGAGGAGGAGGAGGAAGAGGAGGAGGAGATGGAGAGAATGAAGAGACCCAAGCCAAAAATCATCCA
  GACTCGGAGGCCAGAGTACACACCCATCCACCTCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214962 representing NM_153724
  Red=Cloning site Green=Tags(s)
  
  MHFRDFNYNFSSLIACVANGDVFSESETRAKFESLFRTYDKDITFQYFKSFKRVRINFSNPLSAADARLQ
  LHKTEFLGKEMKLYFAQTLHIGSSHLAPPNPDKQFLISPPASPPVGWKQVEDATPVINYDLLYAISKLGP
  GEKYELHAATDTTPSVVVHVCESDQEEEEEEEEEMERMKRPKPKIIQTRRPEYTPIHLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153724
• ORF Size: 597 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153724.2, NP_714946.2
• RefSeq Size: 2165 bp
• RefSeq ORF: 600 bp
• Locus ID: 266766
• UniProt ID: Q6IN33
• Cytogenetics: 11q11
• MW: 22.9 kDa
• Gene Summary: Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development.[UniProtKB/Swiss-Prot Function]