Lipa (NM_012732) Rat Tagged ORF Clone

CAT#: RR214675

Lipa (Myc-DDK-tagged ORF) - Rat lipase A, lysosomal acid, cholesterol esterase (Lipa), (10 ug)

Product Images

Circular map for RR214675

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lipa
• Synonyms: Chole; Chole2; LAL; Lip1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214675 representing NM_012732
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACTGCTAGGCCGGGTGATCTGTTTCGTCGTTGGGATCCTGCTTTCTGGGGGCCCAACGGGGACCA
  TATCAGCTGTGGACCCTGAAGCAAACATGAATGTGACTGAGATAATCATGCACTGGGGATATCCTGGCGA
  AGAGCACTCAGTACAGACAGGAGATGGCTACATTCTGGGTGTTCACCGAATCCCGCATGGGCGGAAGAAC
  CAGTTTGATAAAGGTCCCAAACCAGTTGTGTATCTTCAGCATGGCTTCCTGGCAGATTCTAGTAACTGGG
  TCACAAACATTGACAACAACAGCCTGGGCTTCATCCTGGCAGATGCTGGCTTTGATGTGTGGATGGGAAA
  CAGCAGAGGAAACACCTGGTCTCGGAAGCACAAGACTCTGTCAGTTTCTCAGGATGAATACTGGGCCTTC
  AGTTTTGATGAGATGGCAAAATATGACCTCCCTGCTTCAATTAACTACATTTTGAATAAAACTGGCCAAG
  AACAGCTGTATTATGTGGGCCACTCTCAAGGCTGCACCATAGGTTTTATAGCATTTTCACAGATGCCTGA
  ACTGGCAAAGAAGGTTAAAATGTTTTTTGCCCTGGCTCCTGTGTTGTCTCTCAATTTTGCTTCGGGCCCG
  ATGGTCAAATTGGGACGGTTGCCGGATCTGCTTCTTGAGGACTTGTTTGGGCAGAAGCAATTTCTTCCTC
  AGAGTGCAATGGTGAAATGGCTAAGCACTCACATTTGCACGCACGTCATCATGAAGGAGCTTTGCGCCAA
  CATCTTCTTTCTGATATGTGGCTTCAATGAGAAGAATTTAAACATGTCTAGAGTGGATGTGTATACAACA
  CACTGTCCTGCGGGAACCTCTGTGCAAAACATGGTACACTGGACCCAGGTTGTTAAATACCATAAGCTTC
  AAGCTTTTGACTGGGGAAGCAGTGACAAGAATTATTTTCATTACAATCAGAGTTATCCACCCTTGTACAG
  CATAAAAGACATGCAACTACCCACTGCTTTATGGAGCGGGGGCAAGGACTGGCTGGCAGACACCAGTGAC
  ATCAATATCTTACTGACTGAAATCCCCACGTTAGTGTACCACAAGAACATTCCCGAGTGGGACCATCTGG
  ATTTTATCTGGGGTTTGGATGCCCCCTGGAGGCTGTATAATGAAGTAGTCAGTCTAATGAAGAAATACCA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214675 representing NM_012732
  Red=Cloning site Green=Tags(s)
  
  MQLLGRVICFVVGILLSGGPTGTISAVDPEANMNVTEIIMHWGYPGEEHSVQTGDGYILGVHRIPHGRKN
  QFDKGPKPVVYLQHGFLADSSNWVTNIDNNSLGFILADAGFDVWMGNSRGNTWSRKHKTLSVSQDEYWAF
  SFDEMAKYDLPASINYILNKTGQEQLYYVGHSQGCTIGFIAFSQMPELAKKVKMFFALAPVLSLNFASGP
  MVKLGRLPDLLLEDLFGQKQFLPQSAMVKWLSTHICTHVIMKELCANIFFLICGFNEKNLNMSRVDVYTT
  HCPAGTSVQNMVHWTQVVKYHKLQAFDWGSSDKNYFHYNQSYPPLYSIKDMQLPTALWSGGKDWLADTSD
  INILLTEIPTLVYHKNIPEWDHLDFIWGLDAPWRLYNEVVSLMKKYQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012732
• ORF Size: 1191 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012732.3, NP_036864.3
• RefSeq Size: 3230 bp
• RefSeq ORF: 1194 bp
• Locus ID: 25055
• Cytogenetics: 1q53
• MW: 45.1 kDa
• Gene Summary: hydrolase that is involved in intracellular degradation of cholesteryl esters and triglycerides; mutation in gene causes Wolman's disease [RGD, Feb 2006]