Daglb (NM_001107120) Rat Tagged ORF Clone

CAT#: RR214653

Daglb (Myc-DDK-tagged ORF) - Rat diacylglycerol lipase, beta (Daglb), (10 ug)

Product Images

Circular map for RR214653

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Daglb
• Synonyms: RGD1310193
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214653 representing NM_001107120
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGGATGGTGCTGTTCGGCCGGCGCTGGTCCCTAGCTAGCGACGACCTGGTGTTCCCCGGGTCTT
  TTGAGCTGTTCCTGCGTGTGCTGTGGTGGATCGCCAGTCTGACGTTATACCTCATGCACAGAAGGAAGCT
  GGACTGTCCTGGTGGGGTCTTGCTCAGCACGTACCTGATCGTCCTCCTTGTTCTCCTGGCTGTTATTATA
  GGCATCGTGCTGGCCATCGTGTGTGTCAGCATGAGAGGAACCATTTGTAACCCTGGACCTCGGAAGTCTA
  TGTCTAAGCTGCTCTATATCCGACTGGCCCTCTTCCTGCCAGAGATGGTCTGGGCTTCTCTGGGGGCTGC
  CTGGGTGGCAAAAGGTATCCAATGTGACAGGACAGTTGTGATTGGCATCATCGCCACCGTCATTGTCAGT
  TGGATTGTCATCGCTGCCACTATGGTCACCATCGTCTTTGTCTTTGACCCGCTGGGTGGGAAAATGGCTC
  CGTATCCCCCATGCATCCCTGAACACCTGGACAGTAACAGTTCAAACCATTTATTGACCGGCCTGAGGAC
  GGCTGCAAAGAGCGTGTGGGAGACCCGGGTGCAATGCTGCTGCTGCTGCATTGGGCAGGACGACAACACC
  AGAGTCGCCTTTTCCAGCACGGCCGACCTGTTCTCCACCTACTTCTCTGACACAGACCTGGTGCCCAGCG
  ATATTGCAGCCGGCTTCACCCTTCTCCACCAGCAACAGGACAAGATCAGCCACAGCCGGGAGCCTTCGGA
  GGTCGTGACCCACACACCAGGGCAACCTCAGGAAACAGAGCTGGACGCGGAAGTGGAAAACTGCCATCAC
  TACATGCCGTTTGCAGCAGCTGCCTACGGATGGCCCCTCTACATCTACAGGAACCCGTTCACGGGGCTGT
  GCAGGATCGGTGGTGACTGCTGCAGAGGCAGAGACATAGAGTATGATGCCGTGGAAGGTGACGAGCACCA
  CTGCCACTTCGCCTCCATCCTGAAGACGACGGGGCTGCAGTACAGAGACTTCATCCACGTTAGCTTTCAC
  GACAAGGTGTACGAGCTGCCCTTCATAGTGGTTCTGGATCACAGGAAGGAGTCTGTTGTGGTTGCCGTGA
  GAGGGACCATGTCTCTCCAGGACGTCCTGACCGATCTGTCTGCCGAGAGCGAGAACCTAGAGCTGGATAT
  CGAGCTACAGGACTGCGTGGCCCACAAGGGAATTGCTCAAGCGGCCAGATACATTTATCGAAGACTGGTC
  AACGATGGGATTCTTAGCCAAGCCTTCAGTGTTGCTCCGGAGTACCGGCTCGTTGTGGTGGGGCACAGCC
  TGGGAGCTGGAGCTGCGGCCCTGTTAGCCATCATGCTCCGCGGGGCCTACCCACAAGTCCGTGCTTATGC
  CTTCTCCCCGCCCAGGGGGCTGCTGAGCAAATCCCTCTTTGAGTACTCCAAGGACTTCGTGGTGTCGCTG
  ATCCTAGGGATGGATGTGATCCCCAGGTTAAGCGTGGCCAACATGGAGGATCTCAAGAGGAGGATCCTGC
  GAGTGATCGCCAACTGCAATAAGCCAAAGTACAAGATCTTGCTGCACGGCTGTTGGTACAGCGTGTTTGG
  AGGAAGCCCTGATAACTTTCCAACCGAACTGGATGAGGGCAACCAGGGGGCCCTGACTCAGCCTCTTCTA
  GGGGAGCAGACTCTGCTAACACGGTGCTCCCCAGGCTACTGCTCCGGCGACTCCCCACTGGATTCTCCCA
  AGTACCCCACTCTGTATCCACCCGGCAGGATCATCCACCTGGAAGAGGAGGGTGGTTCAGGGAGGTTTGG
  CTGCTGCTCTGCTGCCCAGTACAGAGCGAGGTGGGCACATGAAACAGAATTCAGCAAGATCCTGATAGGC
  CCAAAGATGCTGATTGACCATATGCCCGACGTCATGATTCGGGCCCTGGACAGAGTGGTCGCCGACAGAA
  CAGCCTGTGTCTCGTGCCCTGGCCAAGGCGGCTCTAACGTCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214653 representing NM_001107120
  Red=Cloning site Green=Tags(s)
  
  MPGMVLFGRRWSLASDDLVFPGSFELFLRVLWWIASLTLYLMHRRKLDCPGGVLLSTYLIVLLVLLAVII
  GIVLAIVCVSMRGTICNPGPRKSMSKLLYIRLALFLPEMVWASLGAAWVAKGIQCDRTVVIGIIATVIVS
  WIVIAATMVTIVFVFDPLGGKMAPYPPCIPEHLDSNSSNHLLTGLRTAAKSVWETRVQCCCCCIGQDDNT
  RVAFSSTADLFSTYFSDTDLVPSDIAAGFTLLHQQQDKISHSREPSEVVTHTPGQPQETELDAEVENCHH
  YMPFAAAAYGWPLYIYRNPFTGLCRIGGDCCRGRDIEYDAVEGDEHHCHFASILKTTGLQYRDFIHVSFH
  DKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESENLELDIELQDCVAHKGIAQAARYIYRRLV
  NDGILSQAFSVAPEYRLVVVGHSLGAGAAALLAIMLRGAYPQVRAYAFSPPRGLLSKSLFEYSKDFVVSL
  ILGMDVIPRLSVANMEDLKRRILRVIANCNKPKYKILLHGCWYSVFGGSPDNFPTELDEGNQGALTQPLL
  GEQTLLTRCSPGYCSGDSPLDSPKYPTLYPPGRIIHLEEEGGSGRFGCCSAAQYRARWAHETEFSKILIG
  PKMLIDHMPDVMIRALDRVVADRTACVSCPGQGGSNVP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107120
• ORF Size: 2004 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107120.1, NP_001100590.1
• RefSeq Size: 3019 bp
• RefSeq ORF: 2007 bp
• Locus ID: 304289
• Cytogenetics: 12p11
• MW: 73.8 kDa