Fahd2a (NM_001134834) Rat Tagged ORF Clone

CAT#: RR214646

Fahd2a (Myc-DDK-tagged ORF) - Rat fumarylacetoacetate hydrolase domain containing 2A (Fahd2a), (10 ug)

Product Images

Circular map for RR214646

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fahd2a
• Synonyms: Fahd2; RGD1563674
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214646 representing NM_001134834
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGCTCTGGTAGAAGGAGGTTGCTGTCAACTCTGCTGCAGGTTCAGAAGAGGCCCTGTCAACCCT
  GCAGAAATATGAGACTGGTACAGTTCCAGGCACCTCACCTGGAAGAGCCTCACGTGGGCCTAGAGTCAGG
  GATTGGTGGGGGAGTTGTAGACCTAAACACCTTTGACCCAGCGCTCCCCAAGACAATGGTGCAGTTCCTG
  GAGCGGGGAGAGACTGCCCTTTCGGTGGCAAGAAGAGCCCTGGCTGCCCAGTTGCCACTCATCCCTCGGT
  CACAGGTGACCTTCTTAGCCCCTGTCACCCGGCCAGACAAGGTGATATGTGTGGGCTTGAATTATGCAGA
  CCACTGTCAAGAACAGAACGTGCGGGTGCCCAAGAATCCCATCATCTTCAGCAAGTTCTCCAGCTCCATC
  GTGGGGCCCTATGACAGCATCATCCTCCCACCAGAGAGTAAGGAAGTTGACTGGGAGGTGGAGATGGCCG
  TGGTCATTGGGAAGAAAGGCAAACACATCAAGGCCACAGATGTCATGGCTTACGTGGCTGGCTTCACTGT
  GGCTCATGATGTGAGTGCTCGGGACTGGCAAATGAGAAATGGAAAGCAGTGGTTACTGGGAAAAACTTTT
  GATACTTTCTGCCCCCTGGGCCCTGCCTTGGTTACCAAGGATACCATAGCAGATCCACACAATCTAAAGA
  TCTCCTGTCGAGTGAATGGGGAGATTGTCCAGAGCAGCAACACCAACCAGATGGTGTTCAAGACTGAAGA
  TCTGATAGCCTGGGTCTCCCAGTTTGTCACTCTGTACCCAGGGGACATTCTCCTGACTGGGACTCCTCCA
  GGTGTCGGCATGTTTAGGAAGCCTCCTGTCTTTCTCAAGAAGGGTGATGAAGTCCAGTGTGAGATTGAAG
  AGCTTGGTGTCATCATCAACAAGGTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214646 representing NM_001134834
  Red=Cloning site Green=Tags(s)
  
  MLGSGRRRLLSTLLQVQKRPCQPCRNMRLVQFQAPHLEEPHVGLESGIGGGVVDLNTFDPALPKTMVQFL
  ERGETALSVARRALAAQLPLIPRSQVTFLAPVTRPDKVICVGLNYADHCQEQNVRVPKNPIIFSKFSSSI
  VGPYDSIILPPESKEVDWEVEMAVVIGKKGKHIKATDVMAYVAGFTVAHDVSARDWQMRNGKQWLLGKTF
  DTFCPLGPALVTKDTIADPHNLKISCRVNGEIVQSSNTNQMVFKTEDLIAWVSQFVTLYPGDILLTGTPP
  GVGMFRKPPVFLKKGDEVQCEIEELGVIINKVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134834
• ORF Size: 939 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134834.1, NP_001128306.1
• RefSeq Size: 1257 bp
• RefSeq ORF: 942 bp
• Locus ID: 296131
• UniProt ID: B2RYW9
• Cytogenetics: 3q36
• MW: 34.6 kDa
• Gene Summary: May have hydrolase activity.[UniProtKB/Swiss-Prot Function]