Cdh15 (NM_207613) Rat Tagged ORF Clone

CAT#: RR214507

Cdh15 (Myc-DDK-tagged ORF) - Rat cadherin 15 (Cdh15), (10 ug)

Product Images

Circular map for RR214507

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdh15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214507 representing NM_207613
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCTGCTCTGCTCTTCGCCCTCGGGCTGCTTGCCCAGAGCCTTGGCCTCTCCTGGGCAGTCCCTG
  AGCCTGAACCCAGCACCCTGTACCCCTGGCGCCGGGCATCAGCCCCAGGCCGTGTGCGGAGAGCCTGGGT
  CATCCCACCCATCAGTGTGTCTGAGAACCACAAACGCCTCCCCTACCCACTTGTGCAGATCAAGTCTGAC
  AAACAACAGCTAGGCAGTGTCATCTACAGTATCCAGGGTCCCGGTGTGGACGAGGAGCCCCGAAACGTCT
  TCTCCATCGACAAGTTCACTGGGAGGGTGTACCTCAATGCCACTCTGGACCGTGAGAAGACGGACCGCTT
  CAGGCTGAGGGCCTTTGCCCTGGACTTGGGTGGCTCTACCCTGGAGGACCCCACGGACCTGGAGATCGTC
  GTGGTGGATCAAAATGACAACCGGCCAGTCTTCCTACAGGATGTGTTCAGAGGCCGCATCCTGGAGGGTG
  CCATCCCAGGCACCTTCGTAACCAGGGCTGAGGCCACAGATGCCGACGATCCGGAGACAGACAATGCGGC
  CCTCAGGTTCTCTATCCTGGAGCAGGGCAGCCCTGAGTTGTTCAGCATTGACGAGCACACTGGAGAGATC
  CGCACAGTGCAAGTGGGGCTGGACCGTGAGGTGGTGGCTGTGTACAACCTGACCTTGCAGGTGGCAGACA
  TGTCCGGAGATGGCCTCACCGCCACAGCCTCGGCCATCATCTCCGTAGATGACGTCAACGACAATGCCCC
  CGAGTTCACCAAGGATGAGTTCTTTATGGAGGCTGCAGAGGCTGTCAGTGGAGTGGACGTGGGACGGCTT
  GAGGTAGAAGACAAGGATCTGCCCGGTTCCCCCAACTGGGTGGCCAGGTTCACCATCCTGGAAGGCGATC
  CTGACGGGCAGTTCAAGATCTATACGGACCCCAAGACCAATGAAGGTGTGCTGTCCGTGGTCAAGCCCCT
  GGATTATGAGAGCCGTGAGCAGTATGAGCTCAGAGTGTCTGTACAGAACGAGGCCCCTCTGCAGACAGCT
  GCCCCCCGGGCCCAGCGGGGCCAGACCAGGGTCAGTGTGTGGGTTCAGGACACCAACGAGGCTCCAGTGT
  TTCCAGAGAACCCACTGAGGACGAGCATAGCTGAAGGAGCCCCGCCAGGCACCTCTGTGGCCACCTTCTC
  TGCCCGAGACCCTGACACGGAACAGCTGCAGAGAATCAGCTACTCTAAGGACTACGACCCAGAAGACTGG
  CTGCAAGTGGACCGGGCCACAGGCAGGATTCAGACCCAGCGAGTGTTGAGCCCTGCCTCACCCTTTTTAA
  AGGACGGCTGGTACAGGGCCATCATCCTAGCCCTGGACAACGCCATGCCTCCCAGCACAGCCACAGGCAC
  CCTGTCCATTGAGATCTTAGAAGTCAACGACCATGCCCCTGCACTGGCCCCTCCTCTGTCTGGCAGCTTG
  TGCAGTGAACCGGACCAAGGCCCCGGTCTCCTCTTGGGTGCCACGGATGAGGACCTGCCCCCGCACGGGG
  CCCCCTTCCACTTCCAGCTGAACCCCAGGGTACCAGATCTCGGCCGGAACTGGAGCCTCAGCCAGATTAA
  CGTGAGCCATGCACGCTTGCGGCTCCGACACCAGGTCTCTGAGGGCCTGCATCGCCTGAGCCTGCTGCTC
  CAGGACTCTGGGGAGCCGCCCCAGCAGCGAGAGCAAACGCTGAACGTTACCGTGTGTCGCTGTGGGTTGG
  ATGGCACCTGCCTGCCCGGGGCCGCTGCGCTGCAAGGAGGAGGTGTAGGCGTCGGCTTGGGCGCACTGGT
  CATTGTGCTGGCCAGCACCGTGGTCCTACTGGTTCTCATCCTGCTTGCTGCGCTCCGCACACGGTTCCGG
  GGGCAGTCTCGGAGCAAGAGTCTGTTGCATGGGCTTCAGGAGGATCTTCGGGACAACATTCTTAACTACG
  ATGAACAAGGAGGCGGGGAGGAGGACCAGGATGCCTACGACATAAACCAGCTGCGCCACCCAGTGGAACC
  GAAGGCCACCAGCCGCTCTTTGGGCCGGCCACCCCTGCGCAGGGATGCACCCTTCAGCTATGTGCCACAG
  CCACATCGAGTACTTCCTACCAGCCCGTCTGACATCGCCAACTTCATCAGTGACGGCTTGGAGGCTGCGG
  ACAGCGACCCCAGCGTGCCTCCCTATGACACAGCTCTCATCTATGACTACGAGGGAGATGGCTCTGTGGC
  AGGGACCCTGAGCTCCATCCTGTCCAGCCAGGGAGATGAAGACCAGGACTATGACTATCTCCGGGACTGG
  GGCCCCCGCTTTGCTCGGCTGGCGGACATGTATGGGCATCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214507 representing NM_207613
  Red=Cloning site Green=Tags(s)
  
  MSSALLFALGLLAQSLGLSWAVPEPEPSTLYPWRRASAPGRVRRAWVIPPISVSENHKRLPYPLVQIKSD
  KQQLGSVIYSIQGPGVDEEPRNVFSIDKFTGRVYLNATLDREKTDRFRLRAFALDLGGSTLEDPTDLEIV
  VVDQNDNRPVFLQDVFRGRILEGAIPGTFVTRAEATDADDPETDNAALRFSILEQGSPELFSIDEHTGEI
  RTVQVGLDREVVAVYNLTLQVADMSGDGLTATASAIISVDDVNDNAPEFTKDEFFMEAAEAVSGVDVGRL
  EVEDKDLPGSPNWVARFTILEGDPDGQFKIYTDPKTNEGVLSVVKPLDYESREQYELRVSVQNEAPLQTA
  APRAQRGQTRVSVWVQDTNEAPVFPENPLRTSIAEGAPPGTSVATFSARDPDTEQLQRISYSKDYDPEDW
  LQVDRATGRIQTQRVLSPASPFLKDGWYRAIILALDNAMPPSTATGTLSIEILEVNDHAPALAPPLSGSL
  CSEPDQGPGLLLGATDEDLPPHGAPFHFQLNPRVPDLGRNWSLSQINVSHARLRLRHQVSEGLHRLSLLL
  QDSGEPPQQREQTLNVTVCRCGLDGTCLPGAAALQGGGVGVGLGALVIVLASTVVLLVLILLAALRTRFR
  GQSRSKSLLHGLQEDLRDNILNYDEQGGGEEDQDAYDINQLRHPVEPKATSRSLGRPPLRRDAPFSYVPQ
  PHRVLPTSPSDIANFISDGLEAADSDPSVPPYDTALIYDYEGDGSVAGTLSSILSSQGDEDQDYDYLRDW
  GPRFARLADMYGHP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207613
• ORF Size: 2352 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207613.1, NP_997496.1
• RefSeq Size: 2736 bp
• RefSeq ORF: 2355 bp
• Locus ID: 361432
• Cytogenetics: 19q12
• MW: 85.8 kDa
• Gene Summary: mouse homolog may play a role in muscle differentiation [RGD, Feb 2006]