Spag6 (NM_001034960) Rat Tagged ORF Clone

CAT#: RR214057

Spag6 (Myc-DDK-tagged ORF) - Rat sperm associated antigen 6 (Spag6), (10 ug)

Product Images

Circular map for RR214057

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Spag6
• Synonyms: RGD1310892
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214057 representing NM_001034960
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAGCGGCAGGTGCTTCAAGTGTTCGAGCAGTACCAGAAAGCCCGGACGCAATTCGTGCAGATGG
  TGGCAGAGCTGGCGACCAGACCCCAAAACATAGAAACGCTACAGAATGCAGGTGTAATGTCTTTGCTAAG
  GCCGCTTCTTCTGGATGTGGTCCCAACAATTCAACAGACTGCTGCTTTGGCTCTGGGGAGACTGGCGAAT
  TACAATGATGACCTGGCAGAAGCAGTTGTGAAGGGCGACATTCTTCCACAGCTTGTTTATTCATTGGCAG
  AACAAAATCGTTTCTACAAAAAGGCAGCTGCCTTTGTGCTGAGAGCAGTTGGTAAACACTCTCCTCAGCT
  GGCCCAGGCCATAGTTGACTGTGGAGCTCTGGATACACTGGTGATATGCTTGGAGGATTTTGACCCTGGA
  GTCAAGGAGGCTGCAGCCTGGGCACTTGGATATATTGCAAGACACAATACAGAATTATCTCAAGCTGTGG
  TGGATGCAGGAGCAATTCCTCTTTTGGTTCTGTGTATCCAGGAGCCAGAAATTGCTTTGAAAAGGATTGC
  TGCCTCGGCCCTCAGTGATATCTCAAAGCACTCCCCAGAGTTAGCGCAGACAGTGGTGGATGCCGGAGCC
  ATTGCTCACCTAGCCCAGATGATCCTCAACCCTGATGCCAAACTGAAGCGCCAGGTACTCTCAGCTCTCA
  GTCAGATTGCAAAACACTCTGTGGACCTGGCAGAGATGGTCGTTGAAGCAGAAATTTTCCCTGTTGTACT
  AACCTGCCTGAAGGACAAAGATGAATATGTGAAGAAAAATGCTTGCACCTTAATTAGAGAAATTGCAAAA
  CATACGCCTGAGCTCTCCCAGCTGATTGTTAATGCCGGAGGTGTGGCTGCTGTGATTGACTGCATCGGAT
  CCAGCAAAGGGAACATACGGCTCCCTGGCATCATGATGCTGGGCTATGTGGCTGCTCATTCTGAGAACCT
  GGCCATGGCGGTGATCATCTCCAAGGGTGTACCCCAGTTGTCAATCTGCCTGTCAGAGGAACCAGAAGAT
  CATATTAAGGCTGCTGCTGCCTGGGCCCTGGGGCAGCTGGGAAGACACACTCCAGAGCATGCCAGGGCTG
  TAGCTGTCACAAACACTCTGCCAGTTCTCCTGTCTTTGTACATGTCAGCAGAGAGCTCTGAGGACCTGCA
  GCTGAAGAGTAAAAAAGCCATAAAGAACATCCTTCAAAAATGTACCTACCTACCAGCCCTCGAGCCATTC
  TTGTACGATGCACCTCCCAATATCCTGAAACATGTTGTTGGGCAGTTCAGTAAGGTGCTGCCACACGACA
  GCAAAGCCCGACGGCTTTTTGTGACAAGTGGTGGACTTAAAAAGGTCCAGGAGATAAAAGCAGAGCCAGG
  CTCTCTCCTGCAGGAATACATCAACAGCATCAACAATTGCTACCCAGAAGAAATAGTAAGGTATTATTCG
  CCTGGATACTCTGAGACACTTCTGCAGAGGGTGGACAGCTACCAGCCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214057 representing NM_001034960
  Red=Cloning site Green=Tags(s)
  
  MSQRQVLQVFEQYQKARTQFVQMVAELATRPQNIETLQNAGVMSLLRPLLLDVVPTIQQTAALALGRLAN
  YNDDLAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPG
  VKEAAAWALGYIARHNTELSQAVVDAGAIPLLVLCIQEPEIALKRIAASALSDISKHSPELAQTVVDAGA
  IAHLAQMILNPDAKLKRQVLSALSQIAKHSVDLAEMVVEAEIFPVVLTCLKDKDEYVKKNACTLIREIAK
  HTPELSQLIVNAGGVAAVIDCIGSSKGNIRLPGIMMLGYVAAHSENLAMAVIISKGVPQLSICLSEEPED
  HIKAAAAWALGQLGRHTPEHARAVAVTNTLPVLLSLYMSAESSEDLQLKSKKAIKNILQKCTYLPALEPF
  LYDAPPNILKHVVGQFSKVLPHDSKARRLFVTSGGLKKVQEIKAEPGSLLQEYINSINNCYPEEIVRYYS
  PGYSETLLQRVDSYQPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034960
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034960.1, NP_001030132.1
• RefSeq Size: 2417 bp
• RefSeq ORF: 1524 bp
• Locus ID: 360747
• Cytogenetics: 11q23
• MW: 55.3 kDa