Ywhah (NM_013052) Rat Tagged ORF Clone

CAT#: RR213745

Ywhah (Myc-DDK-tagged ORF) - Rat tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide (Ywhah), (10 ug)

Product Images

Circular map for RR213745

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ywhah
• Synonyms: 14-3-3e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213745 representing NM_013052
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACCGAGAGCAGCTGCTGCAGCGGGCGCGACTGGCGGAGCAGGCGGAGCGCTACGACGACATGG
  CCTCCGCCATGAAGGCGGTGACAGAGCTGAATGAACCTCTATCTAATGAAGATAGAAATCTCCTCTCTGT
  GGCCTACAAGAATGTAGTTGGTGCCAGGCGATCTTCTTGGAGGGTTATTAGTAGCATTGAGCAGAAAACC
  ATGGCAGATGGGAATGAGAAGAAGCTGGAGAAAGTCAAAGCCTATCGGGAGAAGATTGAGAAGGAGCTGG
  AGACAGTTTGCAATGATGTCTTGGCTCTGCTCGACAAGTTCCTTATCAAGAACTGCAATGATTTTCAGTA
  CGAGAGCAAGGTGTTCTACCTGAAAATGAAGGGCGATTACTACCGCTACCTGGCAGAGGTGGCTTCTGGG
  GAGAAGAAAAACAGTGTGGTTGAAGCTTCTGAGGCAGCGTATAAGGAAGCCTTCGAAATCAGCAAAGAGC
  ACATGCAGCCAACACACCCCATCCGGCTTGGCCTGGCCCTCAATTTTTCTGTGTTCTACTATGAGATCCA
  GAATGCACCAGAGCAGGCCTGCCTCTTAGCCAAACAAGCCTTCGATGATGCTATAGCTGAGCTGGACACA
  TTAAACGAGGATTCCTATAAGGACTCCACTCTCATCATGCAGTTGCTGCGAGACAACCTCACCCTCTGGA
  CGAGCGACCAGCAGGATGAAGAAGCCGGAGAAGGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213745 representing NM_013052
  Red=Cloning site Green=Tags(s)
  
  MGDREQLLQRARLAEQAERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSIEQKT
  MADGNEKKLEKVKAYREKIEKELETVCNDVLALLDKFLIKNCNDFQYESKVFYLKMKGDYYRYLAEVASG
  EKKNSVVEASEAAYKEAFEISKEHMQPTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDT
  LNEDSYKDSTLIMQLLRDNLTLWTSDQQDEEAGEGN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013052
• ORF Size: 738 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013052.1, NP_037184.1
• RefSeq Size: 1689 bp
• RefSeq ORF: 741 bp
• Locus ID: 25576
• UniProt ID: P68511
• Cytogenetics: 14q21
• MW: 28.2 kDa
• Gene Summary: belongs to the 14-3-3 family of proteins; mediates signal transduction via activation of protein kinase C and calcium/calmodulin-dependent protein kinase II [RGD, Feb 2006]