Fads2 (NM_031344) Rat Tagged ORF Clone

CAT#: RR213635

Fads2 (Myc-DDK-tagged ORF) - Rat fatty acid desaturase 2 (Fads2), (10 ug)

Product Images

Circular map for RR213635

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fads2
• Synonyms: Fadsd6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213635 representing NM_031344
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAGGGAGGTAACCAGGGAGAGGGGAGCACCGAGCTCCAGGCTCCGATGCCCACCTTCCGCTGGG
  AGGAGATTCAGAAGCACAACCTGCGCACCGACCGGTGGCTCGTCATCGACCGCAAGGTCTACAACGTTAC
  CAAATGGTCCCAGCGGCACCCAGGGGGGCACCGTGTCATCGGACACTATTCGGGAGAAGATGCTACGGAT
  GCCTTCCGTGCCTTCCACCTGGACCTGGATTTCGTGGGCAAGTTCTTGAAGCCCCTGCTGATTGGTGAGC
  TGGCCCCAGAGGAGCCCAGCCTGGACCGGGGCAAAAGCTCTCAGATCACCGAGGACTTCAGGGCCCTGAA
  GAAGACTGCTGAGGACATGAACCTTTTCAAAACCAACCACCTGTTCTTCTTTCTCCTCCTGTCCCACATC
  ATCGTCATGGAAAGCATCGCCTGGTTCATCCTCTCGTACTTCGGCAATGGCTGGATTCCCACCGTCATCA
  CGGCCTTTGTCCTTGCTACCTCCCAGGCCCAAGCTGGATGGCTACAACATGATTATGGCCACCTTTCTGT
  CTATAAGAAATCCATATGGAACCACATTGTCCACAAGTTTGTCATTGGCCACTTAAAGGGTGCCTCCGCC
  AACTGGTGGAACCATCGACATTTCCAGCACCATGCGAAGCCCAACATCTTCCACAAGGACCCCGACATAA
  AGAGCCTGCACGTGTTTGTCCTTGGAGAGTGGCAGCCCCTCGAGTATGGCAAGAAGAAGCTGAAATATCT
  GCCCTACAACCACCAGCATGAATACTTCTTCCTGATCGGACCACCGCTGCTCATCCCTATGTACTTCCAG
  TACCAGATCATCATGACCATGATCAGACGCAGAGACTGGGTGGACTTGGCCTGGGCCATCAGCTACTATG
  CACGTTTCTTCTACACCTATATCCCTTTCTATGGCATCTTGGGAGCCCTGGTTTTCCTCAACTTTATCAG
  GTTCCTGGAGAGCCACTGGTTTGTGTGGGTCACACAGATGAACCACATTGTCATGGAGATTGATCTTGAT
  CACTACCGGGACTGGTTCAGCAGCCAGCTGGCAGCCACCTGCAATGTGGAGCAGTCCTTCTTCAATGACT
  GGTTCAGCGGGCACCTCAATTTCCAGATTGAGCACCACCTCTTCCCCACTATGCCAAGACACAACTTGCA
  CAAGATTGCCCCACTGGTGAAGTCTCTCTGCGCCAAGCATGGCATTGAATACCAAGAGAAGCCGCTGCTG
  AGGGCCCTGCTCGACATTGTGAGTTCACTGAAGAAGTCTGGGGAGCTGTGGCTGGATGCCTACCTCCACA
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213635 representing NM_031344
  Red=Cloning site Green=Tags(s)
  
  MGKGGNQGEGSTELQAPMPTFRWEEIQKHNLRTDRWLVIDRKVYNVTKWSQRHPGGHRVIGHYSGEDATD
  AFRAFHLDLDFVGKFLKPLLIGELAPEEPSLDRGKSSQITEDFRALKKTAEDMNLFKTNHLFFFLLLSHI
  IVMESIAWFILSYFGNGWIPTVITAFVLATSQAQAGWLQHDYGHLSVYKKSIWNHIVHKFVIGHLKGASA
  NWWNHRHFQHHAKPNIFHKDPDIKSLHVFVLGEWQPLEYGKKKLKYLPYNHQHEYFFLIGPPLLIPMYFQ
  YQIIMTMIRRRDWVDLAWAISYYARFFYTYIPFYGILGALVFLNFIRFLESHWFVWVTQMNHIVMEIDLD
  HYRDWFSSQLAATCNVEQSFFNDWFSGHLNFQIEHHLFPTMPRHNLHKIAPLVKSLCAKHGIEYQEKPLL
  RALLDIVSSLKKSGELWLDAYLHK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031344
• ORF Size: 1332 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031344.2, NP_112634.1
• RefSeq Size: 1706 bp
• RefSeq ORF: 1335 bp
• Locus ID: 83512
• UniProt ID: Q9Z122
• Cytogenetics: 1q43
• MW: 52.4 kDa
• Gene Summary: Acts as a fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 6 of the fatty acyl chain. Involved in biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors. Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleate (GLA) (18:3n-6) and stearidonate (18:4n-3), respectively (PubMed:10049752, PubMed:14563830, PubMed:22216341, PubMed:11988075). Subsequently, in the biosynthetic pathway of HUFA n-3 series, desaturates tetracosapentaenoate (24:5n-3) to tetracosahexaenoate (24:6n-3), which is then converted to docosahexaenoate (DHA)(22:6n-3), an important lipid for nervous system function (PubMed:11988075). Also desaturates (11E)-octadecenoate (trans-vaccenoate)(18:1n-9), a metabolite in the biohydrogenation pathway of LA (18:2n-6) (PubMed:24070791).[UniProtKB/Swiss-Prot Function]