Dvl1 (NM_031820) Rat Tagged ORF Clone

CAT#: RR213018

Dvl1 (Myc-DDK-tagged ORF) - Rat dishevelled, dsh homolog 1 (Drosophila) (Dvl1), (10 ug)

Product Images

Circular map for RR213018

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dvl1
• Synonyms: dvl-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213018 representing NM_031820
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGACCAAAATCATCTACCACATGGACGAGGAGGAGACGCCGTACCTGGTCAAGCTGCCCGTAG
  CTCCCGAGCGCGTCACGCTGGCAGACTTCAAGAACGTGCTCAGCAACCGGCCGGTGCACGCCTACAAATT
  CTTCTTCAAGTCTATGGACCAGGACTTCGGGGTGGTGAAGGAGGAGATCTTCGACGACAATGCCAAGTTG
  CCCTGCTTCAATGGCCGTGTGGTTTCCTGGCTGGTCCTGGCTGAGGGCGCTCACTCGGATGCAGGGTCCC
  AGGGCACTGACAGCCACACGGACCTGCCCCCACCCCTTGAGAGGACAGGCGGCATTGGGGACTCCAGGCC
  CCCCTCCTTCCATCCAAATGTTGCCAGTAGCCGAGATGGAATGGACAATGAGACAGGCACAGAGTCAATG
  GTCAGTCACCGGCGGGAGCGAGCCCGACGTCGAAACCGTGATGAGGCTGCCCGGACCAATGGGCACCCAA
  GAGGGGACCGGCGGCGGGAGCTGGGACTACCTCCAGACAGTGCGTCCACTGTACTGAGCAGTGAGCTTGA
  ATCTAGCAGCTTTATTGACTCAGATGAGGAGGACAACACGAGCCGGCTGAGCAGCTCTACAGAGCAGAGT
  ACCTCCTCTCGGCTAATTCGTAAGCACAAATGCCGTCGTCGGAAGCAGCGCTTGAGGCAGACAGACCGGG
  CATCCTCCTTCAGCAGCATCACCGACTCTACCATGTCCCTGAACATCATCACTGTCACGCTCAACATGGA
  GAGGCACCACTTCCTGGGCATCAGCATCGTGGGCCAGAGCAACGACCGGGGTGATGGCGGCATCTACATT
  GGATCCATCATGAAGGGCGGGGCTGTGGCTGCTGATGGCCGCATTGAGCCAGGCGACATGTTGCTGCAGG
  TGAATGATGTCAACTTTGAGAACATGAGCAACGATGATGCCGTGCGGGTGCTTCGGGAGATCGTATCCCA
  GACGGGGCCCATCAGTCTCACAGTGGCCAAGTGCTGGGACCCAACCCCTCGGAGCTACTTCACCATCCCA
  AGGGCTGACCCAGTGCGACCCATCGACCCGGCTGCCTGGCTGTCCCACACAGCAGCACTGACCGGTGCTC
  TGCCCCGCTATGGTACGAGTCCCTGCTCCAGCGCCATCACACGCACCAGCTCTTCCTCACTAACCAGCTC
  AGTGCCTGGCGCCCCACAGCTTGAGGAGGCACCACTGACTGTGAAGAGTGACATGAGTGCCATTGTCCGC
  GTCATGCAGTTGCCAGACTCGGGACTGGAGATCCGGGACCGCATGTGGCTTAAGATCACCATTGCCAATG
  CTGTCATTGGGGCGGATGTGGTGGACTGGCTGTACACACACGTGGAGGGCTTCAAGGAGCGAAGGGAGGC
  GAGGAAGTATGCCAGCAGCATGCTGAAGCATGGTTTCCTGCGGCACACGGTGAACAAGATCACTTTCTCT
  GAGCAGTGCTACTATGTCTTTGGTGACCTGTGCAGCAACCTCGCATCCCTGAACCTCAACAGTGGCTCCA
  GTGGAGCCTCAGATCAGGACACACTGGCCCCACTGCCCCACCCATCTGTACCCTGGCCCTTGGGTCAAGG
  CTATCCCTACCAGTACCCAGGACCCCCGCCCTGCTTCCCACCTGCTTACCAGGACCCTGGCTTCAGCTAT
  GGCAGTGGCAGTGCTGGGAGTCAGCAGAGTGAAGGGAGCAAGAGCAGTGGGTCCACACGGAGCAGCCATC
  GCACCCCAGGCCGAGAGGAGCGCCGGGCAACTGGAGCTGGGGGTAGTGGCAGTGAATCAGACCACACAGT
  ACCAAGTGGGTCTGGTAGCACTGGCTGGTGGGAGCGGCCTGTCAGCCAGCTTAGCCGTGGCAGTAGCCCT
  CGAAGTCAGGCTTCAGCTGTTGCCCCAGGGCTCCCTCCACTGCACCCCCTTACCAAGGCCTATGCAGTAG
  TGGGTGGGCCACCTGGAGGGCCACCTGTCCGGGAGCTGGCTGCTGTTCCTCCAGAACTTACAGGTAGCCG
  CCAGTCCTTCCAAAAGGCCATGGGAAACCCCTGTGAGTTCTTTGTGGACATCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213018 representing NM_031820
  Red=Cloning site Green=Tags(s)
  
  MAETKIIYHMDEEETPYLVKLPVAPERVTLADFKNVLSNRPVHAYKFFFKSMDQDFGVVKEEIFDDNAKL
  PCFNGRVVSWLVLAEGAHSDAGSQGTDSHTDLPPPLERTGGIGDSRPPSFHPNVASSRDGMDNETGTESM
  VSHRRERARRRNRDEAARTNGHPRGDRRRELGLPPDSASTVLSSELESSSFIDSDEEDNTSRLSSSTEQS
  TSSRLIRKHKCRRRKQRLRQTDRASSFSSITDSTMSLNIITVTLNMERHHFLGISIVGQSNDRGDGGIYI
  GSIMKGGAVAADGRIEPGDMLLQVNDVNFENMSNDDAVRVLREIVSQTGPISLTVAKCWDPTPRSYFTIP
  RADPVRPIDPAAWLSHTAALTGALPRYGTSPCSSAITRTSSSSLTSSVPGAPQLEEAPLTVKSDMSAIVR
  VMQLPDSGLEIRDRMWLKITIANAVIGADVVDWLYTHVEGFKERREARKYASSMLKHGFLRHTVNKITFS
  EQCYYVFGDLCSNLASLNLNSGSSGASDQDTLAPLPHPSVPWPLGQGYPYQYPGPPPCFPPAYQDPGFSY
  GSGSAGSQQSEGSKSSGSTRSSHRTPGREERRATGAGGSGSESDHTVPSGSGSTGWWERPVSQLSRGSSP
  RSQASAVAPGLPPLHPLTKAYAVVGGPPGGPPVRELAAVPPELTGSRQSFQKAMGNPCEFFVDIM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031820
• ORF Size: 2085 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031820.1, NP_114008.1
• RefSeq Size: 2955 bp
• RefSeq ORF: 2088 bp
• Locus ID: 83721
• UniProt ID: Q9WVB9
• Cytogenetics: 5q36
• MW: 75.4 kDa
• Gene Summary: mouse homolog contributes to social and sensorimotor behavior [RGD, Feb 2006]