Fgfr4 (NM_001109904) Rat Tagged ORF Clone

CAT#: RR212875

Fgfr4 (Myc-DDK-tagged ORF) - Rat fibroblast growth factor receptor 4 (Fgfr4), (10 ug)

Product Images

Circular map for RR212875

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fgfr4
• Synonyms: MGC116290
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212875 representing NM_001109904
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTGCTGTTGGCTTTGTTGAGCATCTTTCAGGAGACACCAGCCTTCTCCCTTGAGGCCTCTGAGG
  AAATGGAACAGGAGCCCTGCCCAGCCCCAATCTCGGAGCAGCAAGAGCAGGTGTTGACTGTGGCCCTTGG
  GCAGCCTGTGCGGCTATGCTGTGGCCGCACTGAGCGTGGTCGTCATTGGTACAAGGAGGGCAGCCGTTTA
  GCATCTGCTGGGAGAGTACGGGGCTGGAGAGGCCGCCTGGAGATCGCCAGCTTCCTTCCTGAGGATGCTG
  GCCGGTACCTCTGCCTGGCCCGTGGCTCCATGACTGTCGTACACAATCTTACTTTGATTATGGATGACTC
  CTTACCCTCCATCAATAACGAGGACCCCAAGACCCTCAGCAGCTCCTCGAGTGGGCACTCCTACCTGCAG
  CAAGCACCTTACTGGACACACCCCCAACGCATGGAGAAGAAACTGCACGCGGTACCTGCCGGGAACACTG
  TCAAATTCCGCTGTCCAGCTGCAGGGAACCCCATGCCCACCATCCACTGGCTCAAGAACGGACAGGCCTT
  CCACGGAGAGAATCGTATCGGAGGCATTCGGCTGCGTCACCAACACTGGAGCCTCGTGATGGAGAGCGTG
  GTGCCCTCAGACCGTGGCACGTACACGTGTCTTGTGGAGAACTCTCTGGGTAGCATTCGCTACAGCTATC
  TGCTGGATGTGCTGGAGCGGTCCCCGCACCGGCCCATCCTGCAGGCGGGACTCCCAGCCAACACCACGGC
  TGTGGTTGGCAGCAACGTGGAGCTGCTGTGCAAGGTGTACAGTGACGCCCAGCCGCACATCCAGTGGCTG
  AAGCACATCGTTATCAACGGCAGCAGTTTCGGCGCTGATGGTTTCCCCTACGTACAAGTCCTGAAGACAA
  CAGACATCAATAGCTCAGAGGTGGAGGTGCTGTATCTGAGGAACGTGTCGGCTGAGGATGCAGGGGAGTA
  CACCTGCCTGGCGGGCAACTCCATCGGCCTCTCCTACCAGTCAGCGTGGCTCACAGTGCTACCCGCAGAG
  GAAGAAGACCTCGCGTGGACAACAGCAACATCGGAGGCCAGATATACAGATATTATCCTATATGTATCTG
  GCTCACTGGCTTTGGTTTTGCTCCTGCTGCTGGCCGGGGTGTATCACCGACAAGCAATCCACGGCCACCA
  CTCTCGACAGCCTGTCACTGTACAGAAGCTGTCCCGGTTCCCTTTGGCCCGGCAGTTCTCCTTGGAGTCG
  AGGTCCTCTGGCAAGTCAAGTTTGTCCCTGGTGCGAGGTGTCCGGCTCTCCTCCAGTGGCCCGCCCTTGC
  TCACGGGCCTTGTGAGTCTAGACCTACCTCTCGATCCACTTTGGGAGTTCCCCCGGGACAGGCTGGTGCT
  CGGAAAGCCCCTGGGTGAGGGCTGCTTTGGGCAAGTGGTTCGTGCAGAAGCCCTTGGCATGGATTCCTCC
  CGGCCAGACCAAACCAGCACCGTGGCTGTGAAGATGCTGAAAGACAATGCCTCCGACAAGGATTTGGCAG
  ACCTGATCTCTGAGATGGAGATGATGAAGCTAATCGGAAGACACAAGAACATCATTAACCTGCTGGGTGT
  CTGCACTCAGGAAGGGCCCCTGTATGTGATTGTGGAATATGCGGCCAAGGGAAACCTTCGGGAATTCCTC
  CGTGCCCGGCGTCCCCCAGGCCCTGATCTCAGCCCTGATGGGCCTCGGAGCAGCGAAGGACCGCTCTCCT
  TCCCGGCCCTGGTCTCCTGTGCCTACCAGGTGGCCCGAGGCATGCAGTATCTGGAGTCTCGGAAGTGCAT
  CCACCGGGACCTGGCTGCCCGAAACGTGCTGGTGACCGAGGATGACGTGATGAAGATCGCTGACTTTGGT
  CTGGCCCGTGGTGTCCACCACATCGACTACTATAAGAAAACCAGCAATGGCCGCCTGCCAGTCAAGTGGA
  TGGCTCCTGAGGCGTTGTTTGACCGTGTATACACACACCAGAGTGACGTGTGGTCCTTCGGGATCCTGCT
  GTGGGAAATCTTCACCCTCGGGGGCTCCCCATACCCCGGCATCCCAGTGGAGGAGCTGTTCTCACTGCTG
  CGAGAGGGGCACAGGATGGAGCGGCCCCCAAACTGCCCCTCAGAGCTGTATGGGCTAATGAGGGAGTGTT
  GGCACGCAGCTCCTTCTCAGAGGCCGACTTTTAAGCAGCTGGTGGAAGCTCTGGACAAGGTCCTGCTGGC
  TGTCTCTGAAGAGTACCTTGACCTCCGCCTGACCTTTGGACCCTATTCCCCCAACAATGGGGATGCCAGC
  AGCACGTGCTCCTCCAGCGACTCGGTTTTCAGCCACGACCCTTTGCCCCTCGAGCCAAGCCCCTTCCCAT
  TTCCTGAGGCGCAGACCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212875 representing NM_001109904
  Red=Cloning site Green=Tags(s)
  
  MWLLLALLSIFQETPAFSLEASEEMEQEPCPAPISEQQEQVLTVALGQPVRLCCGRTERGRHWYKEGSRL
  ASAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMTVVHNLTLIMDDSLPSINNEDPKTLSSSSSGHSYLQ
  QAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPMPTIHWLKNGQAFHGENRIGGIRLRHQHWSLVMESV
  VPSDRGTYTCLVENSLGSIRYSYLLDVLERSPHRPILQAGLPANTTAVVGSNVELLCKVYSDAQPHIQWL
  KHIVINGSSFGADGFPYVQVLKTTDINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPAE
  EEDLAWTTATSEARYTDIILYVSGSLALVLLLLLAGVYHRQAIHGHHSRQPVTVQKLSRFPLARQFSLES
  RSSGKSSLSLVRGVRLSSSGPPLLTGLVSLDLPLDPLWEFPRDRLVLGKPLGEGCFGQVVRAEALGMDSS
  RPDQTSTVAVKMLKDNASDKDLADLISEMEMMKLIGRHKNIINLLGVCTQEGPLYVIVEYAAKGNLREFL
  RARRPPGPDLSPDGPRSSEGPLSFPALVSCAYQVARGMQYLESRKCIHRDLAARNVLVTEDDVMKIADFG
  LARGVHHIDYYKKTSNGRLPVKWMAPEALFDRVYTHQSDVWSFGILLWEIFTLGGSPYPGIPVEELFSLL
  REGHRMERPPNCPSELYGLMRECWHAAPSQRPTFKQLVEALDKVLLAVSEEYLDLRLTFGPYSPNNGDAS
  STCSSSDSVFSHDPLPLEPSPFPFPEAQTT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109904
• ORF Size: 2400 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109904.1, NP_001103374.1
• RefSeq Size: 3164 bp
• RefSeq ORF: 2403 bp
• Locus ID: 25114
• UniProt ID: Q498D6
• Cytogenetics: 17p14
• MW: 88.7 kDa
• Gene Summary: fibroblast growth factor receptor subtype expressed in lung and kidney [RGD, Feb 2006]