Snx8 (NM_001105912) Rat Tagged ORF Clone

CAT#: RR212742

Snx8 (Myc-DDK-tagged ORF) - Rat sorting nexin 8 (Snx8), (10 ug)

Product Images

Circular map for RR212742

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Snx8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212742 representing NM_001105912
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGGCCGCGCGATGGACCCGCTGCCCTCGCCGGCAGTCGCTGCAGCAGCTGAGGCGGAGGCTGACG
  AGGAGGCGGATCCGCCGGCGACAGGCCCACAAACATCCCAGGTCACCGAGTGGAGGAACCTGGACCCCAG
  GCGAATGCAGATGCCTCAGGGGAATCCGTTGCTGCTGTCCTACACCCTGCAGGAGCTGCTGGCCAAGGAC
  ACGGTGCAGGTGGAGCTCATCCCTGAGAAGAAGGGCCTGTTCCTTAAGCATGTGGAGTACGAGGTTTCCA
  GCCAGCGCTTCAAGTCCTCCGTGTACAGACGCTACAATGACTTCGTGGTCTTCCATGAAGTGCTGCTTCA
  CAAGTTCCCGTACCGCATGGTACCAGCCCTTCCACCCAAGAGAGTGCTGGGAGCCGACCGGGAGTTCATC
  GAGGGCCGCCGGAGGGCCCTGAAGCGCTTCATCAACCTGGTGGCCCGGCACCCGCCCTTCTCTGAGGATG
  TGCTCCTGAAGCTCTTCCTCTCCTTCAGTGGCTCAGATGTTCAGCACAAGCTCAAGGAGGCGGCCCAGTG
  CGTGGGAGATGAGTTCACGAACTGTAAGCTGGCTGCTAGGGCCAAGGACTTCCTCCCAGCTGACATCCAG
  ACTCAATTTGCCATGAGCCGGGAGCTGATTCGAAATGTCTACAACAGCTTCTACAAACTTCGAGACAGAG
  CCGAGCGGATTGCGTCCCGGGCCATTGACAACGCTGCTGATCTTCTCATATTCGGGAAGGAGCTCAGGCA
  AGTGACTCTTGCTTTGGGTTCCGACACCACCCCTCTGCCCTCCTGGGCTGCTCTGCACCTCAGCACGTGG
  GGCTCCCTGAAGCAAGCGCTCAAGGGCCTGTCCGTGGAGTTTGCTTTGCTTGCAGACAAAGCTGCACAGC
  AGGGTAAGAAGGAGGAGAATGATGTGGTGGAGAAGCTGAACCTCTTCTTGGATTTGCTGCAGTCCTACAA
  AGACCTGTGTGAGCGGCATGAGAAGGGCGTGCTTCACAAGCATCAGCGAGCACTGCACAAGTACGGCCTG
  ATGAAGAGGCAGATGATGAGTGCGGCACATGGCCGAGAGCCCGAGTCTGTGGAGCAGCTGGAGTCACGAA
  TTGTGGAGCAGGAGAACGTGATCCAGACTATGGAACTCCGAAACTACTTCTCCCTGTACTGTCTGCACCA
  GGAGACTCAGCTTGTCCATGTCTACCTGCCTCTCACCTCTCACATCCTGGGGGCCTTCGTGAACTCCCAG
  ATCCAAGGGCACAAGGAGATGAGCAAGGTGTGGAATGACCTGAAGCCAAAGCTGAGCTGCCTCTTTGCTG
  GACCTCACAGTGTGCTGACACCACCACGCTCCCCGCAGGAGGATGGCATGTGTCCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212742 representing NM_001105912
  Red=Cloning site Green=Tags(s)
  
  MTGRAMDPLPSPAVAAAAEAEADEEADPPATGPQTSQVTEWRNLDPRRMQMPQGNPLLLSYTLQELLAKD
  TVQVELIPEKKGLFLKHVEYEVSSQRFKSSVYRRYNDFVVFHEVLLHKFPYRMVPALPPKRVLGADREFI
  EGRRRALKRFINLVARHPPFSEDVLLKLFLSFSGSDVQHKLKEAAQCVGDEFTNCKLAARAKDFLPADIQ
  TQFAMSRELIRNVYNSFYKLRDRAERIASRAIDNAADLLIFGKELRQVTLALGSDTTPLPSWAALHLSTW
  GSLKQALKGLSVEFALLADKAAQQGKKEENDVVEKLNLFLDLLQSYKDLCERHEKGVLHKHQRALHKYGL
  MKRQMMSAAHGREPESVEQLESRIVEQENVIQTMELRNYFSLYCLHQETQLVHVYLPLTSHILGAFVNSQ
  IQGHKEMSKVWNDLKPKLSCLFAGPHSVLTPPRSPQEDGMCPH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105912
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105912.1, NP_001099382.1
• RefSeq Size: 2617 bp
• RefSeq ORF: 1392 bp
• Locus ID: 288504
• Cytogenetics: 12q11
• MW: 52.7 kDa