Ncf2 (NM_001100984) Rat Tagged ORF Clone

CAT#: RR212626

Ncf2 (Myc-DDK-tagged ORF) - Rat neutrophil cytosolic factor 2 (Ncf2), (10 ug)

Product Images

Circular map for RR212626

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ncf2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212626 representing NM_001100984
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTAGCTGAGGCCATCAGGCTCTGGAATGAGGGGGTGCAGGCAGCCGACAAGAAGGACTGGAAGG
  GTGCCTTGGAGGCCTTCAGCGAGGTGCAGGACCCCCACTCGAGGATTTGCTTCAACATAGGCTGCATGTA
  CACCATCCTGGACAACCTGCAGGAGGCTGAGCAGGCCTTCACCAAAAGCATCAACAGAGACAAGCACTTG
  GCAGTGGCTTACTTCCAGCGAGGAATGCTCTACTACAGCATGGAGAAGTACCGTCCAGCTTCTGTGGGTA
  GGAAGGCTGCCTTGCTCTTTCTGGGCAGTTATAACTTAGTAGCTAGAATAATTGTTGGTTATCCCCTCAG
  TCCTGGGAAAGTGTTGTATAACATCGCTCTCATGCATGCCAAGAAAGAGGAATGGAAAAAAGCAGAAGAG
  CAGTTAGCATTGGCAACAAACATGAAGTCTGAGCCCAGGCATTCCAAGATTGACAAGGCCATGGAAAGCA
  TCTGGAAGAGGTGTCCTACCTCCCATCTCCCTCTGGATCCTCCACAAGTGACAATGGCTCTGTGGTTTGA
  GGAAGGAGGAGTGGGCAAACGGAGCGTTGTAGCCTCTGTGGTTCACCAAGACAATTTTTCTGGCTTCGCC
  CCTCTGCAGCCACAGTCAGCAGAGCCTCCGCCCAGACCCAAAACCCCAGAGATCTTCAGGGCTCTGGAAG
  GCGAGGCACATCGTGTGTTGTTTGGCTTTGTGCCGGAGACGCCGGAAGAACTACAGGTCATGCCCGGGAA
  CATCGTCTTTGTCTTGAAGAAGGGCAGTGATAACTGGGCCACCGTCATGTTCAACGGACAGAAGGGGCTT
  GTCCCTTGCAACTACCTGGAGCCCGTTGAGCTTCGAATTCACCCTCAGTCGCAGCCTCAGGAAGATACCT
  CCCTGGAATCCGATATTCCACCACCTCCTAACTCTAGTCCCCCAGAAAGACTCCAGTTGTCACCAGGATG
  GTGCCAACAGCTCGGCCCACTAAGGTGTCCTCCCTTCCTCCTCCACCAGGAAGTGAAGCGCAGCGTGCCC
  ATGCCTTACATGCTCAAGGTGCATTACAAATACACAGTGGTCATGGAGACTCAGCTTGGCCTCCCCTACA
  GCCAGCTTCGGAACATGGTGTCTAAGAAGCTGGAGCTCTTGCCAGAACACACTAAACTGAGCTACCAGCG
  TCGGGACAGCCCGGAGCTGCTGCTCCTGTCAGAAGAAAGCATGAAGGATGCCTGGGCCCAAGTGAAAAAC
  TACTGCCTGACCCTCTGGTGTGAGCACACAGTGGGTGATCAAGGTTTTGTAGATGAACCCAAGGAAAAGG
  AAAACTCTGATGCCGATAACCGGACAACAGAGCCTCAGCCTAAGGAAGGGACCCAGGTGGTAGCGATCTT
  CAGTTATGATGCTACCCAGCCAGAAGACCTGGAGTTTGTGGAAGGAGATGTGATCTTGGTGCTATCACAT
  GTGAATGAAGAATGGCTGGAAGGGGAGTGTAAAGGGAAAATTGGCATTTTCCCTAAAGCTTTTGTTGAAG
  GATGTGCAGCCAAGAATTTGGAAGGCACTCCCAGAGAAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212626 representing NM_001100984
  Red=Cloning site Green=Tags(s)
  
  MSLAEAIRLWNEGVQAADKKDWKGALEAFSEVQDPHSRICFNIGCMYTILDNLQEAEQAFTKSINRDKHL
  AVAYFQRGMLYYSMEKYRPASVGRKAALLFLGSYNLVARIIVGYPLSPGKVLYNIALMHAKKEEWKKAEE
  QLALATNMKSEPRHSKIDKAMESIWKRCPTSHLPLDPPQVTMALWFEEGGVGKRSVVASVVHQDNFSGFA
  PLQPQSAEPPPRPKTPEIFRALEGEAHRVLFGFVPETPEELQVMPGNIVFVLKKGSDNWATVMFNGQKGL
  VPCNYLEPVELRIHPQSQPQEDTSLESDIPPPPNSSPPERLQLSPGWCQQLGPLRCPPFLLHQEVKRSVP
  MPYMLKVHYKYTVVMETQLGLPYSQLRNMVSKKLELLPEHTKLSYQRRDSPELLLLSEESMKDAWAQVKN
  YCLTLWCEHTVGDQGFVDEPKEKENSDADNRTTEPQPKEGTQVVAIFSYDATQPEDLEFVEGDVILVLSH
  VNEEWLEGECKGKIGIFPKAFVEGCAAKNLEGTPREV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100984
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100984.1, NP_001094454.1
• RefSeq Size: 1584 bp
• RefSeq ORF: 1584 bp
• Locus ID: 364018
• UniProt ID: A7E3N2
• Cytogenetics: 13q21
• MW: 59.6 kDa
• Gene Summary: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).[UniProtKB/Swiss-Prot Function]