Prkn (NM_020093) Rat Tagged ORF Clone

CAT#: RR212553

Park2 (Myc-DDK-tagged ORF) - Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2), (10 ug)

Product Images

Circular map for RR212553

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prkn
• Synonyms: Park; Park2; Prkn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212553 representing NM_020093
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAGTGTTTGTCAGGTTCAACTCCAGCTATGGCTTCCCAGTGGAGGTCGATTCTGACACCAGCATCT
  TCCAGCTCAAGGAAGTGGTTGCTAAGCGACAGGGGGTTCCAGCTGACCAGCTGCGAGTGATTTTTGCTGG
  GAAGGAGCTGCAGAATCACCTGACAGTACAGAACTGTGACCTGGAACAACAGAGTATCGTTCACATAGTA
  CAGAGACCACAGAGGAAAAGTCACGAAACAAATGCCTCTGGAGGGGACAAACCCCAGAGCACCCCGGAGG
  GCTCCATATGGGAGCCCAGAAGCTTGACTCGAGTGGACCTCAGCAGCCATATCCTGCCAGCGGACTCCGT
  GGGGCTGGCAGTCATTCTGGACACAGACAGCAAGAGTGACTCAGAAGCAGCCAGAGGTCCAGCAGCTAAA
  CCCACCTACCACAGCTTTTTTGTCTACTGCAAAGGCCCCTGCCACAAGGTCCAGCCTGGGAAACTCCGAG
  TTCAGTGCGGCACCTGCAGACAAGCAACCCTCACCTTGGCCCAGGGCCCATCTTGCTGGGATGATGTCTT
  AATTCCAAACCGGATGAGTGGAGAGTGCCAATCTCCAGACTGCCCTGGGACAAGAGCTGAATTTTTCTTT
  AAATGTGGAGCACACCCAACCTCAGACAAGGACACATCAGTAGCTTTGAACCTGATCACCAACAACAGCC
  GCAGCATCCCCTGCATCGCGTGCACGGATGTCAGGAACCCTGTCTTGGTCTTCCAATGTAACCACCGCCA
  CGTGATCTGTTTGGACTGCTTCCACTTGTACTGTGTCACAAGGCTCAACGATCGGCAGTTTGTCCACGAC
  GCTCAACTTGGCTACTCCCTGCCGTGTGTGGCTGGCTGTCCCAACTCCCTGATTAAAGAGCTCCATCACT
  TCAGGATCCTTGGAGAAGAGCAGTACAACAGGTACCAGCAGTATGGTGCCGAGGAGTGCGTGCTGCAGAT
  GGGAGGTGTGCTGTGCCCCCGTCCTGGCTGCGGAGCTGGGCTGCTGCCTGAACAGGGCCAGAGGAAAGTC
  ACCTGCGAAGGGGGCAACGGCCTGGGCTGTGGGTTCGTTTTCTGCCGGGACTGCAAGGAAGCATACCATG
  AAGGGGAGTGCGACTCGATGTTCGAAGCCTCGGGGGCGACTTCTCAGGCATACCGGGTGGATCAAAGAGC
  TGCTGAGCAAGCACGGTGGGAGGAGGCCTCCAAGGAAACCATCAAGAAAACCACCAAGCCTTGTCCTCGC
  TGCAATGTGCCCATTGAAAAGAATGGAGGATGTATGCACATGAAGTGTCCTCAGCCCCAGTGCAAGCTGG
  AGTGGTGCTGGAACTGTGGCTGTGAGTGGAACCGAGCCTGCATGGGTGATCACTGGTTTGACGTGAGTGG
  TGCTGGAACTGTGGCTGTGAGTGGAACCGAGCCTGCATGGGTGATCACTGGTTTGACGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212553 representing NM_020093
  Red=Cloning site Green=Tags(s)
  
  MIVFVRFNSSYGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFAGKELQNHLTVQNCDLEQQSIVHIV
  QRPQRKSHETNASGGDKPQSTPEGSIWEPRSLTRVDLSSHILPADSVGLAVILDTDSKSDSEAARGPAAK
  PTYHSFFVYCKGPCHKVQPGKLRVQCGTCRQATLTLAQGPSCWDDVLIPNRMSGECQSPDCPGTRAEFFF
  KCGAHPTSDKDTSVALNLITNNSRSIPCIACTDVRNPVLVFQCNHRHVICLDCFHLYCVTRLNDRQFVHD
  AQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRYQQYGAEECVLQMGGVLCPRPGCGAGLLPEQGQRKV
  TCEGGNGLGCGFVFCRDCKEAYHEGECDSMFEASGATSQAYRVDQRAAEQARWEEASKETIKKTTKPCPR
  CNVPIEKNGGCMHMKCPQPQCKLEWCWNCGCEWNRACMGDHWFDVSGAGTVAVSGTEPAWVITGLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020093
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 1564 bp
• RefSeq ORF: 1398 bp
• Locus ID: 56816
• UniProt ID: Q9JK66
• Cytogenetics: 1q11
• MW: 51.7 kDa
• Gene Summary: localized to the cytoplasm in neurons of many regions of the brain; has N-terminal ubiquitin and ring-IBR-ring finger domains [RGD, Feb 2006]