Mum1l1 (NM_001109321) Rat Tagged ORF Clone

CAT#: RR212348

Mum1l1 (Myc-DDK-tagged ORF) - Rat melanoma associated antigen (mutated) 1-like 1 (Mum1l1), (10 ug)

Product Images

Circular map for RR212348

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mum1l1
• Synonyms: RGD1565148
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212348 representing NM_001109321
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGTGAATATGTCCTATGTAGTTGGAAAGACCAATTATGGCCAGCCAAAGTTTTAGATAGGTCTG
  AAAGTCCTTCAGACAGTAAGAGGAAAAAGACATTGTCTGTAGAAGTTGAAATACTTTCACTGGATGAGAA
  AATTACCGTGGAAAGCAAAGACACAAAAGTCCTAACCAAATCTGCTGTCGAAGCCATTATGTCCTCTCTA
  ACAGTGCAGTCAGAGGTCAGCTTTACACCTACGGAGGAAACTGCCTATGAAAGATCACTGAAAATGGCAC
  TGGAAATCGTGGAAGAAAGAACAAATCAGAGCCACGAAAGCATGGCAGAAGAGCAACATATGGCTACAGC
  ATCTGAAAATGTGCCACAGCAGCCGCCTGATTCGCCTCCTCATAAAAAGTTCCGGAAACTCGAAAGCAAC
  CTCCAGGAAGACTCGGCTTCCATGTTGCTGTGCTCCGAGAGTGATGATTCCCTCTCCGACGAGAAGTTGC
  AGGTGCACACAACCAGTGAGAACATGCCAAGTGAAGTGGAAATGAAGTCAGAAAACTTAAGCTGCTGCCA
  AACGTACCCTTCCTTTTCAGATGATGATGATAAAAAAGAAGAAAAGAAAAAGATTGACATCTCGGCGATC
  ATGTCTGTGAATTTATCGCTCAAAGAAGAAAGCGAATTTATTAAAGAAGAAAAGTTTGTCCCCTCATCAG
  AAGATCTCGCTGTACCCAAAGAGGAGTCCCAAGACATCCTCCCAGATGCCCCGGCTGTTTCCTCTGAATG
  CTTTATTGTCTCAGAGAATAACATGGAAGATCCTGGAGAGGGCCCATCAAATCAGAATCCAGGTTCCTAT
  GGCAGCCAAAGTCAGTCTACTGTGGAATCAGATGTAGGTGCTGAGACATCCACTGCAGGGTGCTCAGGGG
  ACTATCAGGTTTCCCTTCCTGCCCGTGATACAGTCAACAGTGATCTACTACTCCAGAGACTGGATTTAGA
  AGATCTTGAGGAAGAAGCCCGAGCTTCTGGCAAGCTTTTGTCTCTAAATCCTGCCTGTGCGGCTGCGTTA
  GAAAATGATGATGAGGACGACGATGAAGACCTTCCACGTTTCATCCTCCGTTATGAGACACGTGCATTTG
  AAACCGGCATGATAGTGTGGTTTAAATATCAAAAATACCCATTTTGGCCAGCAGTGATCAAAAGCATCAG
  GCGGAAAGAGAGGAAAGCGAGTGTGCTTTTGGTTGAGGCAGACATGAATCCTCAAAAGAGAGGCGTTAGA
  GTATCTTTGAGAAGGCTGAAAAAATATGACTGTAAAGAGAAACAGGCACTAGTGGAGAAAGCCAGGGAGG
  AGTACAAGGAGAGCATTGATTGGTGCGTGTCGCTGATTTGTGACTACCGAGTTAGACTAGGCTGTGGTTC
  TTTTACTGGTTCATTCTTTGAGTATTACGCTGCTGACATCAGTTACCCGGTCAGGAAAATCATCAAACAA
  GATACGTTCAGAAATATATTTCCAAAGCTATACAATGAAGACGCGGGGGAGCAATTGCCTGTGGCATCTC
  ACGCCAAGAGAGTATCTTTCCGGAAAATTCTCCCTGATCGCATGAAGCCTGCTCGGGACCGAGCTAACAA
  GAACCTTGTAGATTTCATTGTCAATGCAAAAGGAACTGAGGACCACCTCCTGGGAATTTTAAAGGGCACA
  AAAAAATCCAAGTGGCTGAAATCATTTCTGAATGCAAAGAGTTTCACACCCTGTATTGAAACCTACTTTG
  AAGACGAAGATCAACTGGATGAAGTGGTGAAATATCTACAAGAAATTTATAAGCAAATTGACCAGAAGAT
  GCTGACTCTGATAAAAGATGACAAAATTAAGTTTGTCTTGGAAGTTCTTCTACCCGAAGCAATTATTTGC
  TCAATTTCTGCAGTTGATGGCTTAGATTATGAAGCAGCGGAGGCAAAGTATCTAAAGGGACCATCCCTTG
  GCTATAGGGAGAGAGAATTATATGATTCCAAAATCCTATTTGAAAAGAGGCGGAGGTCATTACCAAATGA
  AGGTCGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212348 representing NM_001109321
  Red=Cloning site Green=Tags(s)
  
  MDGEYVLCSWKDQLWPAKVLDRSESPSDSKRKKTLSVEVEILSLDEKITVESKDTKVLTKSAVEAIMSSL
  TVQSEVSFTPTEETAYERSLKMALEIVEERTNQSHESMAEEQHMATASENVPQQPPDSPPHKKFRKLESN
  LQEDSASMLLCSESDDSLSDEKLQVHTTSENMPSEVEMKSENLSCCQTYPSFSDDDDKKEEKKKIDISAI
  MSVNLSLKEESEFIKEEKFVPSSEDLAVPKEESQDILPDAPAVSSECFIVSENNMEDPGEGPSNQNPGSY
  GSQSQSTVESDVGAETSTAGCSGDYQVSLPARDTVNSDLLLQRLDLEDLEEEARASGKLLSLNPACAAAL
  ENDDEDDDEDLPRFILRYETRAFETGMIVWFKYQKYPFWPAVIKSIRRKERKASVLLVEADMNPQKRGVR
  VSLRRLKKYDCKEKQALVEKAREEYKESIDWCVSLICDYRVRLGCGSFTGSFFEYYAADISYPVRKIIKQ
  DTFRNIFPKLYNEDAGEQLPVASHAKRVSFRKILPDRMKPARDRANKNLVDFIVNAKGTEDHLLGILKGT
  KKSKWLKSFLNAKSFTPCIETYFEDEDQLDEVVKYLQEIYKQIDQKMLTLIKDDKIKFVLEVLLPEAIIC
  SISAVDGLDYEAAEAKYLKGPSLGYRERELYDSKILFEKRRRSLPNEGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109321
• ORF Size: 2037 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109321.1, NP_001102791.1
• RefSeq Size: 3796 bp
• RefSeq ORF: 2040 bp
• Locus ID: 501630
• Cytogenetics: Xq32
• MW: 77.2 kDa