Col1a1 (NM_053304) Rat Tagged ORF Clone

CAT#: RR212285

Col1a1 (Myc-DDK-tagged ORF) - Rat collagen, type I, alpha 1 (Col1a1), (10 ug)

Product Images

Circular map for RR212285

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Col1a1
• Synonyms: COLIA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212285 representing NM_053304
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAGCTTTGTGGACCTCCGGCTCCTGCTCCTCTTAGGGGCCACTGCCCTCCTGACGCATGGCCAAG
  AAGACATCCCTGAAGTCAGCTGCATACACAATGGCCTAAGGGTCCCTAATGGTGAGACGTGGAAACCTGA
  TGTATGCTTGATCTGTATCTGCCACAATGGCACGGCTGTGTGCGATGGCGTGCTATGCAAAGAAGACTTG
  GACTGTCCCAACCCCCAAAAACGGGAGGGCGAGTGCTGTCCTTTCTGCCCAGAAGAATATGTATCACCAG
  ACGCAGAAGTCATAGGAGTCGAGGGACCCAAGGGAGACCCTGGCCCCCAAGGCCCACGGGGACCTGTTGG
  CCCCCCTGGACAAGATGGCATCCCTGGACAGCCTGGACTTCCTGGTCCTCCTGGTCCCCCCGGCCCCCCC
  GGACCCCCTGGTCTTGGAGGAAACTTTGCTTCCCAGATGTCCTATGGCTATGATGAGAAATCAGCTGGAG
  TTTCCGTGCCTGGCCCCATGGGTCCTTCTGGTCCTCGTGGTCTCCCTGGCCCCCCTGGTGCACCTGGTCC
  TCAAGGTTTCCAAGGCCCCCCTGGTGAACCTGGCGAGCCTGGCGCTTCAGGTCCAATGGGTCCCCGAGGT
  CCCCCTGGCCCTCCTGGCAAGAACGGAGATGATGGGGAAGCTGGTAAGCCTGGCCGCCCTGGTGAGCGTG
  GACCTCCTGGACCTCAGGGTGCTCGTGGATTGCCTGGAACAGCTGGCCTCCCCGGAATGAAGGGACACAG
  AGGTTTCAGTGGTTTGGATGGTGCCAAAGGAGATACTGGTCCTGCTGGTCCTAAGGGAGAGCCTGGCAGT
  CCTGGTGAAAATGGAGCTCCCGGCCAGATGGGTCCCCGAGGTCTGCCTGGTGAGAGAGGTCGCCCTGGAC
  CCCCTGGCAGTGCTGGTGCTCGTGGTAACGATGGTGCTGTCGGTGCAGCTGGGCCCCCCGGTCCCACCGG
  CCCTACTGGCCCTCCTGGCTTCCCTGGTGCAGCTGGTGCTAAGGGTGAAGCTGGTCCCCAGGGAGCCCGA
  GGCTCTGAAGGTCCCCAGGGTGTGCGTGGTGAGCCCGGACCCCCTGGCCCTGCTGGTGCTGCTGGTCCTG
  CTGGAAACCCTGGTGCTGATGGACAACCTGGTGCTAAAGGTGCCAATGGTGCTCCTGGTATTGCTGGTGC
  TCCTGGCTTCCCTGGTGCCCGAGGCCCCTCTGGACCTCAGGGCCCCAGCGGCGCTCCTGGTCCCAAAGGC
  AACAGTGGTGAACCTGGTGCCCCTGGCAACAAAGGAGACACTGGTGCCAAAGGAGAACCCGGCCCTGCTG
  GAGTTCAAGGTCCCCCTGGCCCTGCTGGAGAAGAAGGAAAACGAGGAGCCCGTGGTGAGCCTGGACCTAG
  CGGCCTGCCCGGACCTCCTGGCGAGCGCGGTGGACCTGGTAGCCGTGGTTTCCCTGGTGCTGATGGTGTT
  GCTGGCCCCAAGGGTCCTGCTGGTGAACGTGGTTCTCCTGGCCCTGCTGGTCCCAAAGGTTCTCCTGGTG
  AAGCTGGTCGCCCTGGTGAAGCTGGTCTCCCTGGTGCCAAGGGTCTCACTGGCAGTCCTGGCAGCCCTGG
  TCCTGATGGCAAAACCGGCCCCCCTGGTCCCGCTGGTCAAGATGGTCGCCCTGGACCCGCAGGTCCTCCT
  GGAGCCCGTGGACAGGCTGGTGTGATGGGATTCCCTGGACCTAAGGGTACTGCTGGAGAACCTGGAAAGG
  CTGGAGAACGAGGTGTCCCCGGACCCCCTGGCGCTGTTGGTCCCGCTGGCAAAGATGGCGAAGCTGGAGC
  TCAAGGAGCCCCCGGCCCTGCTGGTCCTGCTGGTGAGAGAGGTGAACAAGGTCCCGCTGGCTCCCCTGGA
  TTCCAGGGTCTTCCTGGTCCCGCTGGTCCTCCTGGTGAAGCAGGCAAGCCTGGTGAACAGGGTGTTCCTG
  GAGACCTTGGTGCCCCTGGACCCTCTGGCGCAAGAGGCGAGAGAGGTTTCCCTGGTGAACGTGGTGTACA
  AGGTCCCCCAGGTCCTGCTGGTCCCCGAGGAAACAATGGTGCCCCCGGCAACGATGGTGCCAAGGGTGAT
  ACTGGTGCCCCCGGAGCTCCTGGTAGCCAGGGTGCCCCCGGTCTTCAGGGAATGCCTGGTGAACGTGGTG
  CAGCTGGTCTTCCAGGTCCTAAGGGTGACAGAGGTGATGCTGGTCCCAAAGGTGCTGATGGTTCTCCTGG
  CAAAGATGGCGTCCGTGGTCTGACTGGTCCCATTGGTCCTCCTGGCCCTGCTGGTGCCCCTGGTGACAAG
  GGTGAAACTGGTCCCAGTGGTCCTGCTGGCCCCACCGGAGCCCGTGGTGCCCCCGGAGACCGTGGTGAGC
  CTGGTCCCCCTGGTCCTGCTGGCTTCGCTGGCCCCCCTGGTGCTGATGGCCAACCTGGTGCGAAAGGTGA
  ACCTGGTGATACTGGTGTGAAAGGTGACGCTGGTCCTCCTGGCCCTGCTGGTCCCGCTGGACCCCCTGGC
  CCCATTGGTAACGTTGGTGCTCCTGGACCCAAAGGTTCTCGTGGTGCTGCTGGTCCCCCTGGTGCTACTG
  GTTTCCCTGGTGCTGCTGGTCGTGTTGGTCCCCCTGGTCCCTCTGGAAATGCTGGACCCCCTGGCCCTCC
  CGGTCCCGTTGGCAAAGAAGGGGGCAAAGGTCCCCGTGGTGAGACTGGTCCCGCTGGACGTCCTGGTGAA
  GTTGGTCCCCCAGGTCCCCCTGGCCCTGCTGGTGAGAAAGGATCTCCTGGTGCTGATGGACCTGCTGGCT
  CTCCTGGTACCCCTGGACCTCAGGGTATTGCTGGACAGCGTGGTGTGGTCGGTCTTCCCGGTCAGAGAGG
  AGAAAGAGGCTTCCCTGGTCTTCCTGGACCCTCTGGTGAACCCGGCAAACAAGGTCCTTCTGGAGCAAGT
  GGTGAACGTGGTCCCCCTGGCCCTATGGGCCCCCCTGGATTGGCTGGCCCCCCTGGTGAATCTGGTCGTG
  AGGGATCCCCTGGTGCTGAAGGCTCCCCTGGAAGAGATGGTGCTCCTGGTGCCAAGGGTGACCGTGGTGA
  GACTGGCCCTGCTGGCCCCCCTGGTGCTCCTGGTGCTCCTGGTGCTCCCGGCCCTGTTGGTCCTGCTGGC
  AAGAATGGCGACCGTGGTGAGACTGGTCCTGCTGGTCCTGCTGGTCCCATTGGCCCTGCTGGTGCCCGTG
  GTCCTGCTGGACCCCAAGGCCCCCGTGGTGACAAGGGTGAGACAGGCGAACAAGGTGACAGAGGCATAAA
  GGGTCATCGTGGCTTCTCTGGTCTCCAGGGTCCTCCTGGCTCTCCTGGCTCTCCTGGTGAACAAGGCCCC
  TCTGGAGCTTCTGGTCCTGCAGGTCCCCGGGGTCCCCCTGGCTCTGCTGGTTCTCCTGGCAAAGATGGAC
  TCAACGGTCTCCCTGGCCCCATTGGTCCCCCTGGTCCTCGAGGTCGCACTGGCGATAGTGGTCCTGCTGG
  TCCCCCCGGACCTCCTGGACCCCCTGGCCCTCCCGGTCCTCCCAGCGGTGGTTATGACTTCAGCTTCCTG
  CCTCAGCCACCTCAAGAGAAGTCTCAAGATGGTGGCCGTTACTACCGGGCCGATGATGCCAACGTGGTCC
  GTGACCGTGACCTTGAGGTGGACACTACCCTCAAGAGCCTGAGCCAGCAGATTGAGAACATCCGCAGCCC
  TGAGGGCAGCCGCAAGAACCCCGCCCGCACATGCCGTGACCTCAAGATGTGCCACTCTGACTGGAAGAGC
  GGAGAGTACTGGATCGACCCTAACCAAGGCTGCAACCTGGATGCCATCAAGGTCTACTGCAACATGGAGA
  CAGGTCAGACCTGTGTGTTCCCCACTCAGCCCTCTGTGCCTCAGAAGAACTGGTACATCAGCCCAAACCC
  CAAGGAGAAGAAGCATGTCTGGTTTGGAGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGAAGCGAA
  GGTTCCGATCCTGCCGATGTCGCTATCCAGCTGACCTTCCTGCGCCTGATGTCCACCGAGGCCTCCCAGA
  ACATCACCTATCACTGCAAGAACAGCGTAGCCTACATGGACCAACAGACTGGCAACCTCAAGAAGTCCCT
  GCTCCTCCAGGGCTCCAACGAGATCGAGCTCAGGGGCGAAGGCAACAGTCGATTCACCTACAGCACGCTT
  GTGGATGGCTGCACGAGTCACACCGGAACTTGGGGCAAGACAGTCATCGAATACAAAACCACCAAGACCT
  CCCGCCTGCCCATCATCGATGTGGCTCCCTTGGACATTGGTGCCCCAGACCAGGAATTCGGAATGGACAT
  TGGCCCTGCCTGCTTCGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212285 representing NM_053304
  Red=Cloning site Green=Tags(s)
  
  MFSFVDLRLLLLLGATALLTHGQEDIPEVSCIHNGLRVPNGETWKPDVCLICICHNGTAVCDGVLCKEDL
  DCPNPQKREGECCPFCPEEYVSPDAEVIGVEGPKGDPGPQGPRGPVGPPGQDGIPGQPGLPGPPGPPGPP
  GPPGLGGNFASQMSYGYDEKSAGVSVPGPMGPSGPRGLPGPPGAPGPQGFQGPPGEPGEPGASGPMGPRG
  PPGPPGKNGDDGEAGKPGRPGERGPPGPQGARGLPGTAGLPGMKGHRGFSGLDGAKGDTGPAGPKGEPGS
  PGENGAPGQMGPRGLPGERGRPGPPGSAGARGNDGAVGAAGPPGPTGPTGPPGFPGAAGAKGEAGPQGAR
  GSEGPQGVRGEPGPPGPAGAAGPAGNPGADGQPGAKGANGAPGIAGAPGFPGARGPSGPQGPSGAPGPKG
  NSGEPGAPGNKGDTGAKGEPGPAGVQGPPGPAGEEGKRGARGEPGPSGLPGPPGERGGPGSRGFPGADGV
  AGPKGPAGERGSPGPAGPKGSPGEAGRPGEAGLPGAKGLTGSPGSPGPDGKTGPPGPAGQDGRPGPAGPP
  GARGQAGVMGFPGPKGTAGEPGKAGERGVPGPPGAVGPAGKDGEAGAQGAPGPAGPAGERGEQGPAGSPG
  FQGLPGPAGPPGEAGKPGEQGVPGDLGAPGPSGARGERGFPGERGVQGPPGPAGPRGNNGAPGNDGAKGD
  TGAPGAPGSQGAPGLQGMPGERGAAGLPGPKGDRGDAGPKGADGSPGKDGVRGLTGPIGPPGPAGAPGDK
  GETGPSGPAGPTGARGAPGDRGEPGPPGPAGFAGPPGADGQPGAKGEPGDTGVKGDAGPPGPAGPAGPPG
  PIGNVGAPGPKGSRGAAGPPGATGFPGAAGRVGPPGPSGNAGPPGPPGPVGKEGGKGPRGETGPAGRPGE
  VGPPGPPGPAGEKGSPGADGPAGSPGTPGPQGIAGQRGVVGLPGQRGERGFPGLPGPSGEPGKQGPSGAS
  GERGPPGPMGPPGLAGPPGESGREGSPGAEGSPGRDGAPGAKGDRGETGPAGPPGAPGAPGAPGPVGPAG
  KNGDRGETGPAGPAGPIGPAGARGPAGPQGPRGDKGETGEQGDRGIKGHRGFSGLQGPPGSPGSPGEQGP
  SGASGPAGPRGPPGSAGSPGKDGLNGLPGPIGPPGPRGRTGDSGPAGPPGPPGPPGPPGPPSGGYDFSFL
  PQPPQEKSQDGGRYYRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMCHSDWKS
  GEYWIDPNQGCNLDAIKVYCNMETGQTCVFPTQPSVPQKNWYISPNPKEKKHVWFGESMTDGFQFEYGSE
  GSDPADVAIQLTFLRLMSTEASQNITYHCKNSVAYMDQQTGNLKKSLLLQGSNEIELRGEGNSRFTYSTL
  VDGCTSHTGTWGKTVIEYKTTKTSRLPIIDVAPLDIGAPDQEFGMDIGPACFV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053304
• ORF Size: 4359 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053304.1, NP_445756.1
• RefSeq Size: 5843 bp
• RefSeq ORF: 4362 bp
• Locus ID: 29393
• UniProt ID: P02454
• Cytogenetics: 10q26
• MW: 138 kDa
• Gene Summary: extracellular matrix collagen protein [RGD, Feb 2006]