Slc6a5 (NM_203334) Rat Tagged ORF Clone

CAT#: RR212161

Slc6a5 (Myc-DDK-tagged ORF) - Rat solute carrier family 6 (neurotransmitter transporter, glycine), member 5 (Slc6a5), (10 ug)

Product Images

Circular map for RR212161

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc6a5
• Synonyms: GLYT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212161 representing NM_203334
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTGCAGTGCTCCCAAGGAAATGAATAAACCACCAACCAACATCTTGGAGGCAACGGTGCCGGGCC
  ACCGGGATAGCCCTCGAGCACCTAGGACCAGCCCTGAGCAGGATCTTCCTGCGGCAGCCCCCGCGGCCGC
  TGTCCAGCCGCCACGTGTGCCCAGGTCGGCTTCCACCGGCGCCCAAACTTTCCAGTCTGCGGATGCGAGA
  GCCTGTGAGGCACAGCGGCCTGGAGTAGGGTTTTGTAAACTTAGCAGCCCCCAGGCACAAGCGACCTCTG
  CGGCCCTCCGGGACTTAAGCGAAGGGCACAGCGCACAGGCCAATCCCCCTTCCGGGGCCGCTGGGGCTGG
  CAACGCTTTACACTGCAAGATTCCAGCTCTGCGTGGCCCGGAGGAGGACGAGAACGTGAGTGTGGCCAAG
  GGCACGCTGGAGCACAACAATACCCCACCCGTGGGCTGGGTGAATATGAGCCAGAGCACAGTGGTGTTGG
  GTACCGATGGAATCGCGTCGGTGCTCCCGGGCAGCGTGGCCACCACTACCATTCCGGAGGACGAGCAAGG
  GGATGAGAATAAGGCCAGAGGGAACTGGTCCAGCAAACTGGACTTCATCCTGTCCATGGTGGGGTACGCA
  GTGGGGCTGGGTAATGTTTGGAGGTTTCCCTACCTGGCCTTCCAGAACGGGGGAGGTGCTTTCCTCATCC
  CTTACTTGATGATGCTGGCACTGGCTGGCTTACCTATCTTCTTCCTAGAGGTGTCCCTGGGCCAGTTTGC
  CAGCCAGGGTCCTGTGTCTGTGTGGAAGGCCATCCCAGCTCTGCAGGGCTGTGGCATTGCGATGCTCATC
  ATCTCCGTCCTCATAGCCATCTACTACAACGTCATCATCTGCTACACGCTCTTCTACCTGTTTGCTTCTT
  TTGTGTCTGTGCTGCCCTGGGGATCCTGCAACAACCCGTGGAACACACCAGAATGCAAAGACAAAACCAA
  ACTTTTACTAGATTCCTGTGTTATCGGTGACCATCCCAAGATACAGATCAAGAACTCTACTTTCTGCATG
  ACTGCCTATCCGAACTTGACCATGGTTAACTTCACCAGCCAGGCCAATAAGACATTTGTCAGCGGGAGTG
  AAGAGTACTTCAAGTACTTTGTGCTGAAGATTTCTGCAGGGATTGAATATCCTGGTGAGATCAGGTGGCC
  CTTGCCGTTCTGCCTTTTCCTGGCCTGGGTGATTGTATATGCATCGCTGGCAAAAGGAATTAAGACATCA
  GGAAAAGTGGTGTACTTCACAGCCACCTTCCCTTATGTCGTCCTGGTCATCCTCCTCATTCGAGGGGTCA
  CCCTGCCTGGAGCTGGAGCCGGTATCTGGTACTTCATCACACCTAAGTGGGAGAAACTCACGGATGCCAC
  GGTGTGGAAGGATGCAGCCACTCAGATTTTCTTCTCCCTGTCTGCGGCCTGGGGAGGGCTCATCACTCTT
  TCTTCTTACAACAAATTCCATAACAACTGCTACAGGGACACGTTAATTGTAACCTGCACCAACAGTGCCA
  CTAGCATCTTCGCTGGGTTTGTCATCTTCTCTGTCATTGGCTTCATGGCCAACGAGCGCAAAGTCAACAT
  TGAGAATGTGGCTGACCAAGGGCCAGGCATTGCATTTGTGGTTTACCCAGAAGCCTTAACCAGGCTGCCT
  CTCTCTCCATTCTGGGCCATCATCTTTTTCCTGATGCTTCTCACGCTTGGACTTGACACCATGTTTGCTA
  CCATCGAGACCATTGTGACCTCCATCTCGGATGAGTTTCCCAAGTATCTGCGCACACACAAGCCTGTGTT
  CACCCTGGGCTGCTGCATCTGCTTCTTCATTATGGGCTTCCCAATGATCACACAGGGTGGAATCTACATG
  TTTCAGCTTGTGGACACCTATGCTGCCTCCTATGCTCTTGTCATCATTGCCATATTTGAGCTTGTTGGCA
  TCTCCTATGTGTACGGCTTGCAGAGGTTCTGTGAAGACATCGAGATGATGATTGGATTCCAGCCCAACAT
  TTTCTGGAAGGTCTGCTGGGCGTTTGTCACACCGACCATTTTAACGTTTATCCTTTGCTTCAGCTTCTAT
  CAGTGGGAGCCCATGACCTATGGCTCCTACCGCTACCCTAACTGGTCCATGGTGCTTGGATGGCTGATGC
  TCGCCTGCTCCGTGATCTGGATCCCGATTATGTTCGTGATAAAAATGTATCTGGCTCCTGGGAGATTTAT
  TGAGAGGCTGAAGTTGGTATGCTCGCCACAGCCGGACTGGGGCCCATTCTTAGCTCAGCACCGCGGGGAA
  CGCTACAAGAATATGATCGACCCCTTGGGAACCTCGTCCCTGGGACTCAAGCTGCCAGTGAAGGATTTGG
  AACTGGGCACCCAGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212161 representing NM_203334
  Red=Cloning site Green=Tags(s)
  
  MDCSAPKEMNKPPTNILEATVPGHRDSPRAPRTSPEQDLPAAAPAAAVQPPRVPRSASTGAQTFQSADAR
  ACEAQRPGVGFCKLSSPQAQATSAALRDLSEGHSAQANPPSGAAGAGNALHCKIPALRGPEEDENVSVAK
  GTLEHNNTPPVGWVNMSQSTVVLGTDGIASVLPGSVATTTIPEDEQGDENKARGNWSSKLDFILSMVGYA
  VGLGNVWRFPYLAFQNGGGAFLIPYLMMLALAGLPIFFLEVSLGQFASQGPVSVWKAIPALQGCGIAMLI
  ISVLIAIYYNVIICYTLFYLFASFVSVLPWGSCNNPWNTPECKDKTKLLLDSCVIGDHPKIQIKNSTFCM
  TAYPNLTMVNFTSQANKTFVSGSEEYFKYFVLKISAGIEYPGEIRWPLPFCLFLAWVIVYASLAKGIKTS
  GKVVYFTATFPYVVLVILLIRGVTLPGAGAGIWYFITPKWEKLTDATVWKDAATQIFFSLSAAWGGLITL
  SSYNKFHNNCYRDTLIVTCTNSATSIFAGFVIFSVIGFMANERKVNIENVADQGPGIAFVVYPEALTRLP
  LSPFWAIIFFLMLLTLGLDTMFATIETIVTSISDEFPKYLRTHKPVFTLGCCICFFIMGFPMITQGGIYM
  FQLVDTYAASYALVIIAIFELVGISYVYGLQRFCEDIEMMIGFQPNIFWKVCWAFVTPTILTFILCFSFY
  QWEPMTYGSYRYPNWSMVLGWLMLACSVIWIPIMFVIKMYLAPGRFIERLKLVCSPQPDWGPFLAQHRGE
  RYKNMIDPLGTSSLGLKLPVKDLELGTQC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203334
• ORF Size: 2397 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203334.1, NP_976079.1
• RefSeq Size: 7075 bp
• RefSeq ORF: 2400 bp
• Locus ID: 171148
• UniProt ID: P58295
• Cytogenetics: 1q22
• MW: 87.9 kDa
• Gene Summary: glycine transporter [RGD, Feb 2006]