Scn8a (NM_019266) Rat Tagged ORF Clone

CAT#: RR211948

Scn8a (Myc-DDK-tagged ORF) - Rat sodium channel, voltage-gated, type 8, alpha subunit (Scn8a), (10 ug)

Product Images

Circular map for RR211948

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Scn8a
• Vector: pCMV6-AC-Myc-DDK
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211948 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGCGGCTGCTCGCACCACCAGGCCCTGATAGTTTCAAGCCTTTCACCCCTGAGTCGCTGGCAA
  ACATCGAGAGGCGTATTGCCGAGAGCAAGCTCAAGAAACCACCAAAGGCGGATGGCAGCCACCGGGAGGA
  CGATGAAGACAGCAAGCCCAAGCCAAACAGTGACCTGGAGGCTGGGAAGAGTTTGCCTTTCATCTACGGG
  GACATCCCGCAAGGCCTGGTTGCGGTTCCCCTGGAGGACTTTGACCCTTACTATTTGACGCAGAAAACCT
  TTGTAGTATTAAACAGAGGGAAAACTCTCTTCAGATTTAGTGCCACACCTGCCTTGTACATTTTAAGCCC
  TTTTAACCTGATAAGAAGAATAGCTATTAAAATTTTGATACACTCAGTTTTCAGCATGATCATCATGTGC
  ACCATCCTGACCAACTGTGTGTTCATGACCTTTAGTAACCCTCCAGAATGGTCCAAGAATGTGGAGTACA
  CATTCACAGGGATTTACACATTTGAATCACTAGTGAAAATCATCGCAAGAGGTTTCTGCATAGACGGCTT
  CACCTTCTTGCGAGACCCGTGGAACTGGTTAGACTTCAGTGTCATCATGATGGCATATGTGACAGAGTTT
  GTGGACCTGGGCAATGTCTCAGCGCTGAGAACATTCAGGGTTCTCCGAGCTTTGAAAACTATCTCTGTAA
  TTCCAGGCCTGAAGACAATCGTGGGCGCCCTAATCCAGTCCGTGAAGAAGCTGTCGGACGTGATGATCCT
  GACAGTGTTCTGCCTGAGTGTTTTCGCCCTGATTGGCCTGCAGCTCTTCATGGGGAACCTTCGAAACAAG
  TGTGTCGTGTGGCCCATAAACTTCAACGAGAGCTACCTGGAGAACGGCACCAGAGGCTTTGACTGGGAGG
  AATATATCAACAATAAAACAAACTTTTACATGGTTCCTGGCATGCTAGAACCCTTGCTCTGCGGGAACAG
  TTCTGATGCTGGGCAATGCCCAGAGGGATTCCAGTGCATGAAAGCAGGAAGGAACCCCAACTACGGTTAC
  ACCAGCTTTGACACCTTCAGCTGGGCCTTCTTGGCATTATTCCGCCTTATGACCCAGGACTATTGGGAGA
  ACTTATACCAGCTGACCTTACGAGCCGCTGGGAAAACGTACATGATCTTCTTTGTCTTGGTCATCTTCGT
  GGGTTCTTTCTATCTGGTGAACTTGATCTTGGCTGTGGTGGCCATGGCTTATGAGGAACAGAACCAGGCA
  ACACTGGAGGAGGCAGAGCAAAAAGAGGCCGAGTTCAAGGCAATGCTGGAGCAACTCAAGAAGCAGCAGG
  AGGAGGCACAGGCTGCTGCAATGGCCACCTCAGCGGGCACTGTCTCGGAAGACGCCATTGAAGAAGAAGG
  GGAAGATGGGGTAGGCTCTCCGAGGAGCTCTTCTGAACTGTCTAAACTCAGTTCCAAGAGCGCGAAGGAG
  CGGCGGAACCGACGGAAGAAGAGGAAGCAGAAGGAGCTCTCTGAAGGCGAGGAGAAAGGGGACCCGGAGA
  AGGTGTTTAAGTCAGAGTCGGAAGACGGTATGAGAAGGAAGGCCTTCCGGCTGCCAGACAACAGGATAGG
  GAGGAAGTTTTCCATCATGAATCAGTCGCTGCTCAGCATTCCAGGCTCGCCCTTCCTCTCCCGACATAAC
  AGCAAAAGCAGCATCTTCAGCTTCCGGGGACCCGGTCGGTTCCGGGACCCCGGCTCTGAGAATGAGTTCG
  CAGACGATGAACACAGCACCGTGGAGGAGAGCGAGGGCCGGCGTGACTCGCTCTTCATCCCGATCCGCGC
  CCGCGAGCGCCGCAGCAGCTACAGTGGCTACAGCGGCTACAGCCAGTGCAGCCGCTCGTCGCGCATCTTC
  CCCAGCCTGCGGCGCAGCGTGAAGCGCAACAGCACGGTGGACTGCAACGGCGTAGTGTCACTCATCGGGC
  CCGGCTCACACATCGGGCGGCTCCTGCCTGAGGCAACGACTGAGGTGGAAATTAAGAAGAAAGGCCCTGG
  ATCTCTTTTAGTTTCTATGGACCAACTCGCCTCCTACGGACGGAAGGACAGAATCAACAGCATAATGAGC
  GTGGTCACAAACACGCTAGTGGAAGAGCTGGAAGAGTCTCAGAGAAAGTGCCCACCGTGCTGGTATAAGT
  TTGCCAACACTTTCCTCATCTGGGAGTGTCACCCCTACTGGATAAAACTGAAGGAGATCGTGAACTTAAT
  CGTCATGGACCCTTTTGTAGACTTAGCCATCACCATCTGCATCGTTCTGAATACGCTATTTATGGCAATG
  GAGCACCATCCCATGACACCACAGTTCGAACACGTCTTGGCCGTAGGAAATCTGGTGTTCACCGGGATCT
  TCACGGCGGAAATGTTTCTGAAGCTCATAGCCATGGACCCCTACTATTATTTCCAAGAAGGCTGGAACAT
  TTTTGACGGATTTATTGTCTCCCTCAGTTTAATGGAGCTGAGTCTCGCAGATGTGGAGGGGCTCTCAGTG
  CTGCGGTCTTTCCGACTGCTCCGAGTCTTCAAGCTGGCCAAGTCCTGGCCCACCCTGAACATGCTGATCA
  AGATCATCGGGAACTCCGTGGGTGCCCTGGGCAACCTGACCCTGGTGCTGGCCATCATCGTCTTCATCTT
  CGCCGTGGTGGGGATGCAGCTGTTTGGAAAGAGTTACAAGGAGTGCGTCTGTAAGATCAACCAGGAGTGC
  AAGCTCCCGCGCTGGCACATGAACGACTTCTTCCACTCCTTCCTCATCGTCTTCCGAGTGCTGTGTGGGG
  AGTGGATCGAGACCATGTGGGACTGCATGGAGGTGGCCGGCCAGGCCATGTGCCTCATTGTCTTCATGAT
  GGTTATGGTCATTGGCAACCTGGTGGTGCTGAATCTATTCCTGGCCTTGCTTCTGAGCTCCTTCAGCGCA
  GACAACCTGGCGGCCACAGACGACGACGGGGAAATGAACAACCTGCAGATCTCAGTGATCCGGATCAAGA
  AGGGCGTGGCCTGGACCAAAGTGAAGGTGCACGCCTTCATGCAGGCTCACTTCAAGCAGCGGGAGGCGGA
  TGAAGTGAAACCCCTCGACGAGCTGTATGAGAAGAAGGCCAACTGCATCGCCAACCACACGGGCGTGGAT
  ATCCACCGGAACGGCGACTTCCAGAAGAACGGGAACGGAACCACCAGCGGCATCGGCAGCAGCGTGGAGA
  AGTACATCATCGACGAGGACCACATGTCCTTCATTAACAACCCAAACCTGACCGTCCGGGTGCCCATTGC
  TGTGGGCGAGTCTGACTTCGAGAACCTCAACACAGAGGATGTTAGCAGCGAATCAGACCCTGAAGGCAGC
  AAAGATAAACTGGACGATACCAGCTCCTCAGAAGGAAGTACCATCGACATCAAGCCTGAGGTGGAAGAAG
  TTCCCGTGGAGCAACCTGAGGAATACTTGGATCCGGACGCCTGCTTTACAGAGGGTTGCGTCCAGCGGTT
  CAAGTGCTGCCAGGTCAACATCGAGGAAGGACTAGGCAAGTCGTGGTGGATCTTGCGGAAAACCTGCTTC
  CTCATTGTGGAGCACAATTGGTTTGAGACCTTCATCATCTTCATGATTCTGCTCAGCAGTGGCGCCCTGG
  CCTTTGAGGACATCTACATTGAGCAGAGGAAGACCATCCGCACCATCCTGGAGTATGCGGACAAGGTCTT
  CACCTACATCTTCATCCTGGAGATGTTGCTCAAGTGGACAGCCTACGGCTTCGTCAAGTTCTTCACCAAT
  GCCTGGTGCTGGTTGGACTTCCTCATTGTGGCTGTCTCTTTAGTCAGCCTTATAGCTAATGCCCTGGGCT
  ACTCGGAACTAGGTGCCATAAAGTCCCTTAGGACCCTAAGAGCTTTGAGACCCTTAAGAGCCTTATCACG
  ATTTGAAGGGATGAGGGTGGTGGTGAATGCCTTGGTGGGCGCCATCCCCTCCATCATGAATGTGCTGCTG
  GTGTGTCTCATCTTCTGGCTGATTTTCAGCATCATGGGAGTTAACCTGTTTGCGGGGAAATACCACTACT
  GCTTTAATGAGACTTCTGAAATCCGGTTCGAAATCGATATTGTCAACAATAAAACGGACTGTGAGAAGCT
  CATGGAGGGCAACAGCACGGAGATCCGATGGAAGAATGTCAAGATCAACTTTGACAATGTCGGAGCAGGG
  TACCTGGCCCTTCTTCAAGTGGCAACCTTCAAAGGCTGGATGGACATCATGTATGCGGCTGTAGATTCCC
  GAAAGCCAGACGAGCAGCCTGACTACGAGGGCAACATCTACATGTACATCTACTTCGTCATCTTCATCAT
  CTTCGGCTCCTTCTTCACCCTCAACCTGTTCATCGGTGTCATCATCGACAACTTCAACCAGCAGAAGAAA
  AAGTTTGGAGGTCAGGACATCTTCATGACAGAGGAACAGAAGAAGTACTACAATGCCATGAAAAAGCTGG
  GCTCCAAGAAGCCACAGAAGCCCATCCCCCGACCCTTGAACAAAATCCAAGGGATTGTCTTTGATTTCGT
  CACTCAACAAGCCTTTGACATTGTGATCATGATGCTCATCTGCCTTAACATGGTGACAATGATGGTGGAG
  ACAGACACTCAGAGCAAGCAGATGGAGAACATTCTTTACTGGATTAATCTGGTCTTTGTCATCTTCTTCA
  CCTGCGAGTGTGTGCTCAAAATGTTTGCCTTGAGACACTACTATTTCACCATTGGCTGGAACATCTTTGA
  CTTTGTGGTGGTCATCCTCTCCATTGTGGGAATGTTCCTGGCTGATATCATTGAGAAGTACTTCGTCTCC
  CCAACCCTATTCCGAGTTATCCGATTGGCCCGTATTGGGCGCATCTTGCGTCTGATCAAGGGCGCCAAAG
  GGATCCGCACCCTGCTCTTTGCCTTAATGATGTCGCTGCCCGCCCTGTTCAACATCGGCCTCCTGCTCTT
  CCTCGTCATGTTCATCTTCTCCATTTTTGGCATGTCCAACTTCGCATACGTGAAGCACGAGGCCGGCATT
  GACGACATGTTCAACTTCGAGACATTTGGCAACAGCATGATCTGTTTGTTCCAGATCACAACGTCTGCTG
  GCTGGGATGGCCTGCTGCTGCCAATCCTGAACCGCCCCCCTGACTGCAGCTTGGACAAAGAGCACCCAGG
  GAGTGGCTTCAAAGGGGACTGTGGGAACCCCTCGGTGGGCATCTTCTTCTTTGTGAGCTACATCATCATC
  TCCTTCCTGATTGTGGTGAACATGTACATCGCCATCATCCTGGAGAACTTCAGCGTGGCCACCGAGGAGA
  GCGCCGACCCTCTGAGTGAGGATGACTTCGAGACTTTCTATGAGATCTGGGAGAAGTTTGACCCAGACGC
  CACCCAGTTCATCGAGTACTGTAAGCTGGCAGACTTTGCCGACGCCCTGGAGCACCCGCTCCGAGTACCC
  AAGCCCAACACCATCGAGCTCATCGCCATGGACCTGCCCATGGTGAGCGGAGATCGCATCCACTGCTTGG
  ACATCCTTTTCGCCTTCACCAAGCGAGTCCTGGGAGACAGTGGGGAGTTGGACATCCTGCGGCAGCAGAT
  GGAGGAGCGGTTCGTGGCATCCAATCCTTCCAAAGTGTCTTACGAGCCTATCACAACCACTCTGCGGCGC
  AAGCAGGAGGAGGTGTCTGCAGTGGTCCTGCAGCGTGCCTACAGGGGACACTTGGCTAGGCGGGGCTTCA
  TCTGCAGAAAGATGGCCTCCAACAAGCTGGAGAATGGAGGCACACACAGAGACAAGAAGGAGAGCACCCC
  GTCCACAGCCTCCCTCCCCTCTTACGACAGCGTCACAAAGCCAGACAAGGAGAAGCAGCAGCGTGCGGAG
  GAGGGCAGAAGGGAAAGAGCCAAGAGGCAAAAAGAGGTCAGGGAGTCCAAGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211948 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAARLLAPPGPDSFKPFTPESLANIERRIAESKLKKPPKADGSHREDDEDSKPKPNSDLEAGKSLPFIYG
  DIPQGLVAVPLEDFDPYYLTQKTFVVLNRGKTLFRFSATPALYILSPFNLIRRIAIKILIHSVFSMIIMC
  TILTNCVFMTFSNPPEWSKNVEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYVTEF
  VDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNK
  CVVWPINFNESYLENGTRGFDWEEYINNKTNFYMVPGMLEPLLCGNSSDAGQCPEGFQCMKAGRNPNYGY
  TSFDTFSWAFLALFRLMTQDYWENLYQLTLRAAGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEEQNQA
  TLEEAEQKEAEFKAMLEQLKKQQEEAQAAAMATSAGTVSEDAIEEEGEDGVGSPRSSSELSKLSSKSAKE
  RRNRRKKRKQKELSEGEEKGDPEKVFKSESEDGMRRKAFRLPDNRIGRKFSIMNQSLLSIPGSPFLSRHN
  SKSSIFSFRGPGRFRDPGSENEFADDEHSTVEESEGRRDSLFIPIRARERRSSYSGYSGYSQCSRSSRIF
  PSLRRSVKRNSTVDCNGVVSLIGPGSHIGRLLPEATTEVEIKKKGPGSLLVSMDQLASYGRKDRINSIMS
  VVTNTLVEELEESQRKCPPCWYKFANTFLIWECHPYWIKLKEIVNLIVMDPFVDLAITICIVLNTLFMAM
  EHHPMTPQFEHVLAVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGFIVSLSLMELSLADVEGLSV
  LRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINQEC
  KLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVFMMVMVIGNLVVLNLFLALLLSSFSA
  DNLAATDDDGEMNNLQISVIRIKKGVAWTKVKVHAFMQAHFKQREADEVKPLDELYEKKANCIANHTGVD
  IHRNGDFQKNGNGTTSGIGSSVEKYIIDEDHMSFINNPNLTVRVPIAVGESDFENLNTEDVSSESDPEGS
  KDKLDDTSSSEGSTIDIKPEVEEVPVEQPEEYLDPDACFTEGCVQRFKCCQVNIEEGLGKSWWILRKTCF
  LIVEHNWFETFIIFMILLSSGALAFEDIYIEQRKTIRTILEYADKVFTYIFILEMLLKWTAYGFVKFFTN
  AWCWLDFLIVAVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLL
  VCLIFWLIFSIMGVNLFAGKYHYCFNETSEIRFEIDIVNNKTDCEKLMEGNSTEIRWKNVKINFDNVGAG
  YLALLQVATFKGWMDIMYAAVDSRKPDEQPDYEGNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKK
  KFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKIQGIVFDFVTQQAFDIVIMMLICLNMVTMMVE
  TDTQSKQMENILYWINLVFVIFFTCECVLKMFALRHYYFTIGWNIFDFVVVILSIVGMFLADIIEKYFVS
  PTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKHEAGI
  DDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPPDCSLDKEHPGSGFKGDCGNPSVGIFFFVSYIII
  SFLIVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIEYCKLADFADALEHPLRVP
  KPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGDSGELDILRQQMEERFVASNPSKVSYEPITTTLRR
  KQEEVSAVVLQRAYRGHLARRGFICRKMASNKLENGGTHRDKKESTPSTASLPSYDSVTKPDKEKQQRAE
  EGRRERAKRQKEVRESKC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019266
• ORF Size: 5934 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019266.2, NP_062139.2
• RefSeq Size: 6556 bp
• RefSeq ORF: 5937 bp
• Locus ID: 29710
• UniProt ID: O88420
• Cytogenetics: 7q36
• MW: 225.2 kDa
• Gene Summary: tetrodotoxin-sensitive sodium channel alpha subunit [RGD, Feb 2006]