Krt77 (NM_001008807) Rat Tagged ORF Clone

CAT#: RR211881

Krt77 (Myc-DDK-tagged ORF) - Rat keratin 77 (Krt77), (10 ug)

Product Images

Circular map for RR211881

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Krt77
• Synonyms: Kb39
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211881 representing NM_001008807
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCGCCAGTTCAGTTCTCAGTCTGCATTTAGCTCTAGGAGCAGGAGGGTCTATAGCACCAGGTCTT
  CATCAGGCTTTGGGGGTGGGAGACAGATTCTGGTGTCTGTGGGTCAGTCGAGGAGGTGTGGAGGTGATTA
  TGGTGGTGGGTTCAGTTCCAGGAGTCTCTATAGTCTGGGTGGTAGTAAAAGCATCTTTGGGGGCCTAGTG
  GGGCGAAGTGCCAGTGGTTTCTGCCAGAGTAGAGGGGCAGGAGGAGGATTCGGAGGAGGATTTGGGGGAG
  GAAGAAGCTTTGGTGGAGGTGGCTTTGGGGGTGGCTATGGTGGAGGTGGCAGATTTGGAGGTGGTTTTGG
  GGGCGGATTTGGCACTAGCAATTTTGGGCTTGGTGGCTTCGGTCCTTCTTATCCTCCCGGGGGCATCCAT
  GAGGTGACTGTTAACCAGAGTCTCCTGGAGCCCCTTCACCTAGAAGTGGATCCTGAAATTCAGAAGATCA
  AGACCCAGGAGAGGGAGCAGATCAAGACTCTCAACAACAAGTTTGCCTCCTTCATCGACAAGGTGCGCTT
  CCTGGAGCAGCAGAACCAGGTGCTGCAGACCAAATGGGAGCTGCTGCAGCAGGTGAACACGTCTACTCGG
  ACCAGCAGCCTGGAGCCCATCTTTGAGGAGTTCATCAATCAGCTGCAGAGGCAGGTGGATGTTCTGACCA
  ATGAGCAGTTGCGCCAGAACTCAGAGATCAGGAGCATGCAGGATATCGTGGAGGACTACAAGAACAAGTA
  TGAGGATGAGATCAACAAACGGACCAATTCAGAGAATGACTTTGTTGTTTTGAAAAAGGACGTGGATGCT
  GCTTTCATGGCCAAATCAGACTTGCAGTCCAGAGTGGACACGCTGTACGGGGAGATCAACTTCCTGAAAT
  ATCTATTTGATACGGAGCTGTCCCAGATACAAACCCATGTCAGTGATACCAATGTCATCTTGTCAATGGA
  CAATAACCGCTCCTTGGACTTGGACAGCATCATCAATGCAGTGCGCACCCAATATGAATTAATTGCACAG
  AAGAGCAAAGACGAGGCTGAGGCACTGTACCAAACCAAGTACCAAGAGCTGCAGATCACAGCTGGCAAAC
  ATGGAGATGACCTGAAGAATAGCAAGATGGAGATCTCAGAGCTTAACCGCAATGCCCAGAGGCTGCAGGC
  AGAGATCGCCAACATCAAGAAGCAGATCGAACAGATGCATGGATCCATTTCGGATGCAGAGGAGAGGGGA
  GAAAGAGCTCTCCAGGACGCCAAGCAGAAGCTGCAAGAAACGGAAGAGGCCTTGCAGCAGATGAAAGAGG
  ACCTGGCTAGGCTGCTGAGAGATTACCAGGCACTGCTGGGTGCCAAGCTGTCCTTGGATGTGGAGATCGC
  CACCTACCGCGAGCTTTTGGAGGGCGAGGAGAGCAGGATGTCGGGGGCTCTGCAGAGTCAAGTGAGCATC
  TGGGCTTTGCCCAGCAATGAAGGAAATGATCTTGGTGAACGACTTCACGATCCTCAGTCCCAGGTCCCCG
  TTCCTAAACTGGGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211881 representing NM_001008807
  Red=Cloning site Green=Tags(s)
  
  MSRQFSSQSAFSSRSRRVYSTRSSSGFGGGRQILVSVGQSRRCGGDYGGGFSSRSLYSLGGSKSIFGGLV
  GRSASGFCQSRGAGGGFGGGFGGGRSFGGGGFGGGYGGGGRFGGGFGGGFGTSNFGLGGFGPSYPPGGIH
  EVTVNQSLLEPLHLEVDPEIQKIKTQEREQIKTLNNKFASFIDKVRFLEQQNQVLQTKWELLQQVNTSTR
  TSSLEPIFEEFINQLQRQVDVLTNEQLRQNSEIRSMQDIVEDYKNKYEDEINKRTNSENDFVVLKKDVDA
  AFMAKSDLQSRVDTLYGEINFLKYLFDTELSQIQTHVSDTNVILSMDNNRSLDLDSIINAVRTQYELIAQ
  KSKDEAEALYQTKYQELQITAGKHGDDLKNSKMEISELNRNAQRLQAEIANIKKQIEQMHGSISDAEERG
  ERALQDAKQKLQETEEALQQMKEDLARLLRDYQALLGAKLSLDVEIATYRELLEGEESRMSGALQSQVSI
  WALPSNEGNDLGERLHDPQSQVPVPKLGC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008807
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008807.1, NP_001008807.1
• RefSeq Size: 1560 bp
• RefSeq ORF: 1560 bp
• Locus ID: 406226
• UniProt ID: Q6IG01
• Cytogenetics: 7q36
• MW: 57.3 kDa
• Gene Summary: predicted to be a member of the keratin family of intermediate filament proteins [RGD, Feb 2006]