Abcc3 (NM_080581) Rat Tagged ORF Clone

CAT#: RR211791

Abcc3 (Myc-DDK-tagged ORF) - Rat ATP-binding cassette, sub-family C (CFTR/MRP), member 3 (Abcc3), (10 ug)

Product Images

Circular map for RR211791

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcc3
• Synonyms: Mlp2; Mrp3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211791 representing NM_080581
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGCTTGTGCGGCTCCGGGGAGCTGGGCTCCAAGTTCTGGGACTCCAACCTGACTGTATATACCA
  ACACTCCAGACCTCACACCCTGTTTCCAGAACTCCTTGCTGGCCTGGGTTCCCTGCATCTATCTGTGGGC
  TGCCTTGCCCTGCTACCTGTTCTACCTGAGGCACCATCGGCTTGGCTACATAGTCCTCTCATGCTTATCC
  AGGCTCAAGACGGCCCTCGGTGTTCTGCTGTGGTGTATCTCATGGGTGGACCTGTTCTACTCCTTCCATG
  GCCTGGTTCACGGCTCATCCCCTGCTCCTGTCTTCTTTATCACACCCTTGTTGGTGGGGATCACCATGCT
  GCTGGCCACATTGCTAATCCAGTATGAGCGGCTTCGGGGCGTGCGGTCTTCAGGTGTGCTCATCATCTTC
  TGGCTCCTGTGTGTGATCTGTGCCATCATCCCCTTCCGTTCCAAGATCCTCTTGGCTTTGGCAGAGGGTA
  AAATCTTGGATCCGTTCCGATTCACCACTTTCTACATCTACTTCGCCCTCGTGCTGTGTGCCTTCATCCT
  GTCCTGCTTCCAGGAGAAGCCGCCTTTGTTTTCCCCAGAGAATCTTGACACAAATCCTTGCCCAGAGGCC
  AGCGCTGGCTTCTTCTCCCGTCTGTCTTTCTGGTGGTTCACAAAGCTTGCCATCCTTGGCTACCGACGTC
  CCCTGGAGGACAGTGACCTCTGGTCTCTGTCTGAGGAGGACTGCTCTCACAAGGTGGTACAACGGCTACT
  GGAAGCATGGCAAAAGCAGCAGACCCAAGCATCAGGGCCCCAGACTGCAGCCCTTGAGCCAAAGATCGCA
  GGTGAGGATGAGGTCCTGCTGAAGGCCCGCCCCAAGACCAAGAAGCCTTCCTTTCTGAGGGCTTTGGTGA
  GAACCTTCACCTCCAGCTTGCTCATGGGTGCCTGCTTCAAGCTGATCCAGGACCTGCTCTCCTTCATCAA
  CCCACAGCTGCTCAGCATCCTCATCAGGTTTATTTCTGACCCCACGGCCCCTACCTGGTGGGGCTTCTTG
  CTGGCCGGACTGATGTTCGTGAGCTCCACCATGCAGACATTGATCTTACACCAACATTACCACTGCATCT
  TTGTGATGGCCTTGAGGATACGGACTGCTATCATAGGCGTCATCTACAGGAAGGCTCTGACCATCACCAA
  CTCAGTCAAACGTGAGTACACTGTGGGAGAAATGGTCAACCTCATGTCGGTGGACGCCCAGCGCTTCATG
  GATGTCTCCCCATTCATCAACCTGCTGTGGTCTGCACCTTTGCAGGTCATCCTGGCGATATACTTCCTCT
  GGCAGATCTTAGGCCCATCAGCCCTGGCTGGGGTGGCTGTGATAGTCTTGCTGATACCACTCAATGGAGC
  TGTGTCCATGAAAATGAAGACCTACCAGGTACAGCAAATGAAGTTCAAAGACTCCCGCATCAAACTGATG
  AGTGAGATCCTGAATGGCATCAAAGTTCTGAAGCTGTACGCTTGGGAGCCCACCTTCTTGGAGCAGGTAG
  AAGGAATCAGGCAGGGTGAGCTCCAGCTACTGCGGAAGGGCGCCTACCTGCAGGCTATCTCTACCTTCAT
  CTGGGTCTGCACCCCGTTTATGGTGACCCTGATCACCCTCGGGGTGTACGTGTGTGTGGACAAGAACAAT
  GTGCTGGATGCTGAGAAGGCCTTCGTGTCCCTGTCCTTGTTCAATATCTTAAAGATCCCCCTCAACCTGC
  TGCCTCAGTTAATCAGTGGCATGACCCAGACCAGCGTGTCTCTGAAACGGATCCAGGATTTCCTGAACCA
  AGATGAACTTGACCCCCAGTGTGTGGAAAGAAAGACCATCTCCCCAGGCCGTGCCATCACCATACACAAC
  GGCACCTTCTCCTGGTCCAAGGACCTGCCTCCCACCCTTCACAGCCTAAACATTCAAATCCCGAAGGGGG
  CATTGGTGGCTGTGGTGGGACCTGTGGGCTGTGGGAAGTCTTCCCTGGTGTCTGCCTTGCTCGGAGAGAT
  GGAGAAGCTGGAAGGTGCAGTGTCTGTAAAGGGCTCTGTGGCCTACGTGCCCCAGCAGGCGTGGATCCAG
  AACTGCACACTTCAGGAAAATGTGCTATTTGGCCAACCCATGAACCCCAAGCGCTACCAACAGGCTCTGG
  AGACGTGTGCCCTGCTAGCTGACCTAGATGTGCTTCCTGGTGGGGACCAGACAGAGATTGGAGAGAAGGG
  CATTAACCTATCTGGAGGCCAGCGACAACGGGTGAGTTTGGCCCGAGCTGTTTATAGTGACGCCAACATT
  TTTTTGCTGGATGACCCACTGTCGGCTGTGGACTCTCACGTGGCTAAGCATATCTTCGACCAAGTGATTG
  GACCAGAAGGTGTGCTGGCAGGCAAGACTCGGGTGCTGGTAACCCATGGCATCAGCTTCCTGCCCCAGAC
  GGACTTTATCATTGTGCTTGCTGACGGACAGATTACTGAGATGGGTCACTACTCCGAACTCCTGCAGCAC
  GATGGCTCCTTTGCCAACTTCCTCCGAAACTATGCACCAGATGAAAACCAGGAGGCCAATGAAGGAGTCT
  TGCAACATGCAAATGAGGAGGTGCTCCTGCTTGAAGACACACTCAGCACCCACACAGACCTGACAGACAC
  CGAGCCAGCCATATACGAGGTCCGCAAGCAGTTCATGAGAGAGATGAGCTCCTTGTCTTCTGAAGGAGAG
  GGCCAGAACCGGCCTGTGCTCAAGAGATATACGAGTTCACTGGAGAAGGAGGTGCCGGCGACACAGACTA
  AAGAGACTGGTGCATTAATCAAAGAGGAGATCGCAGAGACAGGCAATGTGAAGCTGAGCGTGTACTGGGA
  TTATGCCAAGTCTGTGGGGCTCTGCACCACGCTGTTTATCTGCCTCCTGTATGCTGGCCAAAATGCGGTT
  GCTATCGGAGCCAATGTGTGGCTCAGTGCCTGGACCAATGATGTGGAGGAACATGGCCAGCAGAACAACA
  CCTCCGTAAGGCTGGGAGTCTACGCCACCCTAGGAATACTGCAAGGGCTCCTGGTCATGCTGTCGGCCTT
  CACCATGGTGGTTGGCGCTATCCAGGCTGCCCGCCTGCTGCACACAGCTCTGTTGCACAACCAGATCCGT
  GCGCCTCAGTCCTTCTTTGACACGACGCCCTCAGGCCGCATCCTCAATCGTTTCTCCAAGGACATATACG
  TCATCGATGAGGTTCTGGCCCCCACCATCCTCATGCTGTTCAATTCATTCTACACATCCATCTCCACCAT
  TGTGGTCATCGTGGCCAGCACGCCACTCTTCTGCGTGGTTGTTCTTCCTCTGGCTGTGTTCTATGGCTTC
  GTGCAGCGCTTCTATGTGGCCACATCGAGGCAGCTGAAGAGACTGGAATCCGTTAGCCGCTCGCCCATCT
  TCTCCCACTTCTCGGAGACAGTAACTGGCACCAGTGTCATTCGGGCCTACGGCCGAGTCCAAGACTTCAA
  GGTCCTCAGTGATGCTAAGGTGGATAGCAACCAGAAGACCACTTATCCTTACATCGCCTCCAACCGGTGG
  CTGGGTGTCCACGTGGAGTTTGTGGGGAACTGCGTGGTGCTCTTCTCCGCGCTGTTTGCAGTGATCGGGA
  GAAACAGCTTGAATCCAGGGCTTGTGGGTCTTTCCGTGTCCTATGCCTTACAGGTGACCTTGAGTTTGAA
  TTGGATGATACGGACGCTATCCGACCTGGAGTCTAATATCATAGCCGTGGAGAGAGTCAAGGAGTACTCT
  AAGACGGAGACTGAGGCTCCCTGGGTGTTGGAGAGCAACCGTGCTCCAGAAGGCTGGCCCAGGAGTGGGG
  TGGTAGAGTTCCGGAACTATTCGGTGCGGTACCGCCCGGGCCTCGAGTTGGTGCTGAAGAATTTGACTCT
  GCATGTGCAGGGTGGGGAGAAGGTAGGCATCGTGGGCCGCACTGGGGCTGGCAAATCTTCCATGACTCTT
  TGCCTATTCCGAATCCTGGAGGCCGCAGAGGGTGAGATCTTCATTGACGGGCTCAATGTGGCACACATTG
  GCCTCCATGACCTGCGTTCACAACTCACCATCATCCCTCAGGACCCCATCCTGTTCTCGGGCACGCTGCG
  CATGAACCTCGATCCCTTTGGCCGTTACTCGGACGAGGACATCTGGAGGACCCTGGAGCTATCCCACCTG
  AGTGCATTTGTGAGCAGCCAGCCGACAGGCCTGGATTTTCAGTGCTCTGAGGGTGGGGATAATCTCAGTG
  TTGGCCAGAGGCAGCTCGTGTGCCTAGCCCGAGCCCTGCTCCGAAAGAGCCGTGTCCTGGTTTTAGACGA
  GGCCACCGCTGCCATTGACCTGGAGACTGATGACCTCATCCAGGGTACCATCCGTACCCAGTTTGAAGAC
  TGCACTGTACTGACCATCGCCCACCGGCTCAACACAATCATGGACTACAACCGGGTCCTGGTCTTGGACA
  AAGGAGTAGTAGCTGAATTTGATTCTCCAGTAAACCTCATTGCAGCTGGAGGCATCTTCTATGGGATGGC
  CAAAGATGCAGGACTCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211791 representing NM_080581
  Red=Cloning site Green=Tags(s)
  
  MDRLCGSGELGSKFWDSNLTVYTNTPDLTPCFQNSLLAWVPCIYLWAALPCYLFYLRHHRLGYIVLSCLS
  RLKTALGVLLWCISWVDLFYSFHGLVHGSSPAPVFFITPLLVGITMLLATLLIQYERLRGVRSSGVLIIF
  WLLCVICAIIPFRSKILLALAEGKILDPFRFTTFYIYFALVLCAFILSCFQEKPPLFSPENLDTNPCPEA
  SAGFFSRLSFWWFTKLAILGYRRPLEDSDLWSLSEEDCSHKVVQRLLEAWQKQQTQASGPQTAALEPKIA
  GEDEVLLKARPKTKKPSFLRALVRTFTSSLLMGACFKLIQDLLSFINPQLLSILIRFISDPTAPTWWGFL
  LAGLMFVSSTMQTLILHQHYHCIFVMALRIRTAIIGVIYRKALTITNSVKREYTVGEMVNLMSVDAQRFM
  DVSPFINLLWSAPLQVILAIYFLWQILGPSALAGVAVIVLLIPLNGAVSMKMKTYQVQQMKFKDSRIKLM
  SEILNGIKVLKLYAWEPTFLEQVEGIRQGELQLLRKGAYLQAISTFIWVCTPFMVTLITLGVYVCVDKNN
  VLDAEKAFVSLSLFNILKIPLNLLPQLISGMTQTSVSLKRIQDFLNQDELDPQCVERKTISPGRAITIHN
  GTFSWSKDLPPTLHSLNIQIPKGALVAVVGPVGCGKSSLVSALLGEMEKLEGAVSVKGSVAYVPQQAWIQ
  NCTLQENVLFGQPMNPKRYQQALETCALLADLDVLPGGDQTEIGEKGINLSGGQRQRVSLARAVYSDANI
  FLLDDPLSAVDSHVAKHIFDQVIGPEGVLAGKTRVLVTHGISFLPQTDFIIVLADGQITEMGHYSELLQH
  DGSFANFLRNYAPDENQEANEGVLQHANEEVLLLEDTLSTHTDLTDTEPAIYEVRKQFMREMSSLSSEGE
  GQNRPVLKRYTSSLEKEVPATQTKETGALIKEEIAETGNVKLSVYWDYAKSVGLCTTLFICLLYAGQNAV
  AIGANVWLSAWTNDVEEHGQQNNTSVRLGVYATLGILQGLLVMLSAFTMVVGAIQAARLLHTALLHNQIR
  APQSFFDTTPSGRILNRFSKDIYVIDEVLAPTILMLFNSFYTSISTIVVIVASTPLFCVVVLPLAVFYGF
  VQRFYVATSRQLKRLESVSRSPIFSHFSETVTGTSVIRAYGRVQDFKVLSDAKVDSNQKTTYPYIASNRW
  LGVHVEFVGNCVVLFSALFAVIGRNSLNPGLVGLSVSYALQVTLSLNWMIRTLSDLESNIIAVERVKEYS
  KTETEAPWVLESNRAPEGWPRSGVVEFRNYSVRYRPGLELVLKNLTLHVQGGEKVGIVGRTGAGKSSMTL
  CLFRILEAAEGEIFIDGLNVAHIGLHDLRSQLTIIPQDPILFSGTLRMNLDPFGRYSDEDIWRTLELSHL
  SAFVSSQPTGLDFQCSEGGDNLSVGQRQLVCLARALLRKSRVLVLDEATAAIDLETDDLIQGTIRTQFED
  CTVLTIAHRLNTIMDYNRVLVLDKGVVAEFDSPVNLIAAGGIFYGMAKDAGLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080581
• ORF Size: 4569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080581.1, NP_542148.1
• RefSeq Size: 5174 bp
• RefSeq ORF: 4572 bp
• Locus ID: 140668
• UniProt ID: O88563
• Cytogenetics: 10q26
• MW: 169.3 kDa
• Gene Summary: human homolog transports conjugated metabolites from hepatocytes into the bloodstream; may play a role in steroid metabolism [RGD, Feb 2006]