Ptch1 (NM_053566) Rat Tagged ORF Clone

CAT#: RR211471

Ptch1 (Myc-DDK-tagged ORF) - Rat patched homolog 1 (Drosophila) (Ptch1), (10 ug)

Product Images

Circular map for RR211471

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptch1
• Synonyms: Ptch; Ptch2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211471 representing NM_053566
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGGCTGGTAACGCCGCCGGGGCCCTGGGCAGGCAGGCCGGCGGCGGGAGGCGCAGACGGACCG
  GGGGACCGCACCGCGCCGCACCGGACCGGGACTATCTGCACCGGCCCAGCTACTGCGACGCCGCCTTCGC
  TCTGGAGCAGATTTCCAAGGGGAAGGCTACTGGCCGGAAAGCGCCGCTGTGGCTGAGAGCGAAGTTTCAA
  AGACTCTTATTTAAACTGGGTTGTTACATTCAAAAGAACTGCGGCAAGTTTTTGGTTGTGGGTCTCCTCA
  TATTTGGGGCCTTCGCTGTGGGATTAAAGGCAGCTAATCTCGAGACCAACGTGGAGGAGCTGTGGGTGGA
  AGTTGGTGGACGAGTGAGTCGAGAATTAAATTATACCCGCCAGAAGATAGGAGAAGAGGCTATGTTTAAT
  CCTCAACTCATGATTCAGACTCCAAAAGAAGAAGGCGCTAATGTCCTGACCACAGAGGCCCTACTACAAC
  ACCTGGACTCAGCACTCCAGGCCAGCCGTGTGCACGTCTACATGTATAACAGGCAATGGAAGTTGGAACA
  TTTATGCTACAAATCAGGGGAACTTATCACAGAGACAGGCTACATGGATCAGATAATAGAATACCTTTAC
  CCTTGCTTAATCATTACACCTTTGGACTGCTTCTGGGAAGGGGCAAAGCTACAGTCCGGGACAGCGTACC
  TCCTAGGTAAGCCTCCTTTACGGTGGACAAACTTTGACCCCTTGGAATTCCTAGAAGAGTTAAAGAAAAT
  AAACTACCAAGTGGACAGTTGGGAGGAAATGCTGAATAAAGCCGAAGTTGGCCATGGGTACATGGACCGG
  CCTTGCCTCAATCCAGCCGACCCAGATTGCCCTGCCACAGCCCCCAACAAAAATTCAACCAAACCTCTTG
  ATGTGGCCCTTGTTTTGAACGGTGGATGTCAAGGTTTATCCAGGAAGTATATGCATTGGCAGGAGGAGTT
  GATTGTGGGTGGCACCGTCAAGAACGCCACTGGAAAGCTTGTCAGCGCTCATGCCCTGCAAACCATGTTC
  CAGTTAATGACTCCCAAGCAAATGTATGAACACTTCAGGGGCTACGACTATGTCTCTCACATCAACTGGA
  ATGAGGATAGGGCTGCCGCCATCCTGGAGGCATGGCAGAGGACTTACGTGGAGGTGGTTCATCAAAGTGT
  TGCCCCAAACTCCACTCAAAAGGTGCTTTCCTTCACCACCACGACCCTGGACGACATCCTAAAATCCTTC
  TCTGATGTCAGTGTCATCCGAGTGGCCAGTGGCTACCTACTGATGCTTGCCTACGCCTGTTTAACCATGC
  TGCGCTGGGACTGCTCCAAGTCCCAGGGTGCCGTGGGGTTGGCTGGTGTCCTGTTGGTTGCACTGTCAGT
  GGCTGCAGGATTGGGCCTCTGCTCCTTGATTGGCATTTCCTTTAATGCTGCAACAACTCAGGTTTTGCCA
  TTTCTTGCCCTTGGTGTTGGTGTGGACGACGTCTTTCTCCTGGCCCATGCATTCAGTGAAACGGGACAGA
  ATAAGAGGATTCCATTTGAGGACAGGACTGGGGAGTGCCTCAAGCGCACGGGAGCCAGCGTGGCCCTCAC
  CTCCATCAGCAATGTCACTGCCTTCTTCATGGCTGCATTAATCCCTATTCCTGCACTGCGAGCGTTCTCC
  CTCCAGGCTGCTGTGGTGGTGGTATTCAATTTTGCCATGGTTCTACTCATTTTCCCTGCAATTCTCAGCA
  TGGATTTATACAGACGTGAGGACAGAAGATTGGATATTTTCTGCTGTTTCACAAGCCCCTGTGTCAGCCG
  GGTGATTCAAGTTGAACCGCAGGCCTACACAGAGCCTCATAGTAACACCCGGTACAGCCCCCCACCCCCG
  TACAGCAGCCACAGCTTCGCCCATGAAACCCACATCACCATGCAGTCCACCGTTCAGCTCCGCACAGAAT
  ATGACCCTCACACACACGTCTACTACACCACCGCCGAGCCGCGCTCTGAGATCTCTGTACAGCCTGTCAC
  CATCACCCAGGACACCCTCAGCTGTCAGAGCCCTGAGAGCACCAGTTCCACCCGGGACCTGCTTTCTCAA
  TTCTCTGACTCCAGCCTCCACTGCCTTGAGCCCCCCTGCACCAAGTGGACACTTTCTTCGTTTGCAGAGA
  AGCACTACGCTCCTTTCCTCTTGAGACCCAAAGCCAAGGTTGTGGTAATCCTTCTTTTCCTGGGCTTGCT
  GGGCGTCAGCCTTTATGGGACCACCCGAGTGAGAGACGGGCTGGACCTCACGGACATTGTCCCCCGGGAA
  ACCAGAGAATATGACTTCATAGCTGCCCAGTTCAAGTACTTCTCTTTCTACAACATGTATATAGTCACCC
  AGAAAGCAGACTACCCAAATATCCAACACCTGCTTTACGACCTTCATAAGAGTTTCAGCAGTGTGAAGTA
  TGTCATGCTGGAGGAGAACAAGCAACTTCCCCAAATGTGGCTGCATTACTTTAGAGACTGGCTCCAAGGA
  CTTCAGGATGCATTTGACAGTGACTGGGAAACCGGGAGGATCATGCCAAACAATTATAAAAATGGATCTG
  ATGATGGGGTCCTCGCTTACAAACTCCTGGTGCAGACTGGCAGCCGAGACAAGCCCATCGACATTAGTCA
  GTTGACTAAACAGCGTCTGGTGGACGCAGATGGCATCATTAATCCGAGCGCTTTCTACATCTACCTGACC
  GCTTGGGTCAGCAATGACCCTGTAGCTTACGCTGCCTCCCAGGCCAACATCCGGCCTCACAGACCAGAGT
  GGGTGCACGACAAAGCTGACTACATGCCAGAGACCAGGCTGAGAATCCCAGCAGCAGAGCCCATAGAGTA
  CGCTCAGTTCCCTTTCTACCTCAACGGCCTACGGGACACCTCAGACTTTGTGGAAGCCATAGAAAAAGTG
  AGAGTCATCTGTAACAACTACACGAGTCTGGGGCTGTCCAGCTACCCCAATGGCTACCCCTTCCTGTTCT
  GGGAGCAGTACATCAGCCTGCGTCACTGGCTGCTGCTAGCCATCAGCGTGGTGCTGGCCTGCACATTTCT
  AGTGTGCGCAGTCTTCCTCCTGAACCCCTGGACGGCCGGGATCATTGTCATGGTCCTGGCTTTGATGACC
  GTTGAGCTCTTCGGCATGATGGGCCTCATTGGGATCAAGCTGAGTGCTGTGCCGGTGGTCATCCTGATTG
  CGTCTGTTGGCATTGGAGTAGAATTCACCGTCCACGTGGCTTTGGCCTTTCTGACAGCCATTGGGGACAA
  GAACCACAGGGCTATGCTCGCCCTGGAGCACATGTTCGCTCCTGTTCTGGACGGTGCTGTGTCCACTCTG
  CTGGGTGTACTGATGCTTGCGGGATCCGAATTTGACTTCATTGTCAGATACTTCTTTGCCGTCCTGGCCA
  TCCTCACCGTCTTGGGAGTTCTCAACGGACTGGTTCTGCTGCCTGTACTCTTATCCTTCTTCGGACCATG
  TCCTGAGGTGTCTCCAGCCAACGGCCTAAACCGGCTGCCCACTCCTTCGCCTGAGCCGCCCCCAAGCGTC
  GTCCGGTTTGCCGTGCCTCCTGGTCACACGAACAATGGGTCTGATTCCTCCGACTCCGAGTACAGCTCTC
  AGACCACGGTGTCTGGCATCAGTGAGGAGCTCAGGCACTATGAAGCACAGCAGGGCACCGGAGGTCCTGC
  CCACCAAGTGATTGTGGAAGCCACAGAAAACCCCGTCTTTGCCCGGTCCACTGTGGTGCATCCTGACTCC
  AGACATCAGCCTCCCTTGAACTCTCGGCAACAGCCCCACCTGGACTCTGGCTCCCTGTCCCCTGGACGGC
  AAGGCCAGCAGCCTCGAAGGGATCCCCCCAGAGAAGGCTTGCGGCCACCCCCCTACAGACCGCGCAGAGA
  CGCTTTTGAAATTTCTACTGAAGGGCATTCTGGCCCTAGCAATAGGGACCGCTCGGGCCCCCATGGGGCC
  CGTTCTCACAACCCTCGGAACCCAACGTCCACTGCCATGGGCAGCTCTGTGCCCAGCTACTGCCAGCCTA
  TCACCACTGTGACGGCTTCCGCTTCTGTAACTGTCGCTGTGCACCCCCCACCTGGACCTGGGCGCAACCC
  CCGAGGGGGACCCTGTCCAGGCTACGAGAGCTACCCCGAGACTGATCACGGGGTGTTCGAGGATCCCCAT
  GTGCCTTTTCATGTCAGGTGTGAGAGGAGGGACTCGAAGGTGGAGGTTATAGAACTACAGGACGTGGAGT
  GTGAGGAGAGGCCGTGGGGGAGCAGCTCCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211471 representing NM_053566
  Red=Cloning site Green=Tags(s)
  
  MASAGNAAGALGRQAGGGRRRRTGGPHRAAPDRDYLHRPSYCDAAFALEQISKGKATGRKAPLWLRAKFQ
  RLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFN
  PQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLY
  PCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDR
  PCLNPADPDCPATAPNKNSTKPLDVALVLNGGCQGLSRKYMHWQEELIVGGTVKNATGKLVSAHALQTMF
  QLMTPKQMYEHFRGYDYVSHINWNEDRAAAILEAWQRTYVEVVHQSVAPNSTQKVLSFTTTTLDDILKSF
  SDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLP
  FLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFS
  LQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTEPHSNTRYSPPPP
  YSSHSFAHETHITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTITQDTLSCQSPESTSSTRDLLSQ
  FSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLRPKAKVVVILLFLGLLGVSLYGTTRVRDGLDLTDIVPRE
  TREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHKSFSSVKYVMLEENKQLPQMWLHYFRDWLQG
  LQDAFDSDWETGRIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLT
  AWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKV
  RVICNNYTSLGLSSYPNGYPFLFWEQYISLRHWLLLAISVVLACTFLVCAVFLLNPWTAGIIVMVLALMT
  VELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNHRAMLALEHMFAPVLDGAVSTL
  LGVLMLAGSEFDFIVRYFFAVLAILTVLGVLNGLVLLPVLLSFFGPCPEVSPANGLNRLPTPSPEPPPSV
  VRFAVPPGHTNNGSDSSDSEYSSQTTVSGISEELRHYEAQQGTGGPAHQVIVEATENPVFARSTVVHPDS
  RHQPPLNSRQQPHLDSGSLSPGRQGQQPRRDPPREGLRPPPYRPRRDAFEISTEGHSGPSNRDRSGPHGA
  RSHNPRNPTSTAMGSSVPSYCQPITTVTASASVTVAVHPPPGPGRNPRGGPCPGYESYPETDHGVFEDPH
  VPFHVRCERRDSKVEVIELQDVECEERPWGSSSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053566
• ORF Size: 4302 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053566.1, NP_446018.1
• RefSeq Size: 4305 bp
• RefSeq ORF: 4305 bp
• Locus ID: 89830
• Cytogenetics: 17p14
• MW: 159.3 kDa
• Gene Summary: human homolog acts as a receptor for sonic hedgehog [RGD, Feb 2006]