Traf6 (NM_001107754) Rat Tagged ORF Clone

CAT#: RR211042

Traf6 (Myc-DDK-tagged ORF) - Rat Tnf receptor-associated factor 6 (Traf6), (10 ug)

Product Images

Circular map for RR211042

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Traf6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211042 representing NM_001107754
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCTCTTAAACTGTGAAAACAGCTGTGCGTCCAGCCAGTCTTCAAGCGACTGCTGTGCTGCCATGG
  CCAACTCCTGCAGTGCTGCCATGAAAGATGACAGTGTGAGTGGCTGTGTCAGCACGGGGAACCTGTCCAG
  CTCCTTCATGGAGGAGATCCAGGGATATGATGTGGAGTTTGACCCACCTTTGGAAAGCAAGTATGAGTGC
  CCCATCTGCTTGATGGCTTTACGGGAAGCAGTGCAAACACCATGTGGCCACAGGTTCTGCAAAGCCTGCA
  TCACCAAGTCCATAAGGGATGCAGGTCACAAGTGCCCAGTTGACAATGAAATACTGCTGGAAAATCAACT
  GTTTCCTGACAATTTTGCAAAGCGAGAGATTCTTTCCCTGACGGTAAAGTGTCCAAATAAAGGCTGTGTG
  CAAAAGATGGAGCTGAGACATCTCGAGGATCATCAAGTACATTGTGAATTCGCTCTAGTGATTTGTCCCC
  AATGCCAACGTTTTTTCCAAAAGTGCCAGATTAATAAACACATTATCGAGGATTGTCCCAGGAGACAGGT
  TTCTTGTGTAAACTGTGCTGTGCCCATGCCGTATGAAGAGAAAGAGATCCACGATCAAAGCTGTCCTCTG
  GCAAATATCATCTGTGAATACTGTGGTACAATCCTCATAAGAGAACAGATGCCTAATCATTATGATCTAG
  ACTGCCCAACAGCTCCAGTCCCCTGCACATTCAGTGTGTTTGGCTGTCACGAAAAGATGCAGAGGAATCA
  CTTGGCACGGCACTTGCAAGAGAACACCCAGTTGCACATGAGACTGTTGGCCCAGGCTGTTCATAATGTT
  AACCTCTCTTTGCGGCCATGCGATGCCTCCTCTCCATCCCGGGGATGTCGTCCTGAGGACCCAAATTATG
  AGGAAACGGTCAAACAGTTGGAGGGGCGCCTAGTAAGACAGGACCATCAAATCCGGGAGCTGACCGCCAA
  AATGGAAACGCAGAGCATGCATGTGAGCGAGCTCAAGCGGACCATTCGAAGCCTCGAGGACAAAGTTGCC
  GAGATGGAAGCACAGCAGTGTAATGGCATTTACATTTGGAAGATTGGCAACTTTGGGATGCACTTGAAAT
  CCCAAGAAGAGGAAAGACCTGTGGTCATTCATAGCCCTGGATTCTACACAGGCAGACCTGGGTACAAGCT
  GTGCATGCGCCTGCACCTCCAGCTACCGACGGCTCAGCGCTGTGCAAACTACATTTCCCTCTTTGTCCAC
  ACAATGCAAGGAGAGTATGACAGCCACCTCCCCTGGCCCTTCCAGGGTACAATACGCCTCACGATCCTTG
  ATCAGTCTGAAGCAGTAATAAGGCAAAACCACGAAGAGGTCATGGATGCTAAGCCAGAACTGCTTGCCTT
  TCAGCGGCCCACCATCCCACGGAACCCCAAAGGTTTTGGCTATGTGACATTCATGCACCTGGAAGCCTTA
  AGACAGGGAACCTTCATCAAGGATGATACGTTATTAGTGCGCTGTGAAGTCTCTACCCGCTTTGACATGG
  GGGGCCTTCGGAAGGAGGGGTTCCAGCCACGGAGTACTGATGCAGGCGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211042 representing NM_001107754
  Red=Cloning site Green=Tags(s)
  
  MSLLNCENSCASSQSSSDCCAAMANSCSAAMKDDSVSGCVSTGNLSSSFMEEIQGYDVEFDPPLESKYEC
  PICLMALREAVQTPCGHRFCKACITKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNKGCV
  QKMELRHLEDHQVHCEFALVICPQCQRFFQKCQINKHIIEDCPRRQVSCVNCAVPMPYEEKEIHDQSCPL
  ANIICEYCGTILIREQMPNHYDLDCPTAPVPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNV
  NLSLRPCDASSPSRGCRPEDPNYEETVKQLEGRLVRQDHQIRELTAKMETQSMHVSELKRTIRSLEDKVA
  EMEAQQCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMRLHLQLPTAQRCANYISLFVH
  TMQGEYDSHLPWPFQGTIRLTILDQSEAVIRQNHEEVMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEAL
  RQGTFIKDDTLLVRCEVSTRFDMGGLRKEGFQPRSTDAGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107754
• ORF Size: 1590 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107754.2, NP_001101224.1
• RefSeq Size: 2753 bp
• RefSeq ORF: 1593 bp
• Locus ID: 311245
• UniProt ID: B5DF45
• Cytogenetics: 3q31
• MW: 60.3 kDa
• Gene Summary: E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Mediates activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production.[UniProtKB/Swiss-Prot Function]