Tmco4 (NM_001034949) Rat Tagged ORF Clone

CAT#: RR210943

Tmco4 (Myc-DDK-tagged ORF) - Rat transmembrane and coiled-coil domains 4 (Tmco4), (10 ug)

Product Images

Circular map for RR210943

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmco4
• Synonyms: MGC124660
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210943 representing NM_001034949
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACATGGAACCGGCCGCACCCAAGGCTGCCTGTGGCACCAGAGCCAGTTGCAGAAGGGGAGTCCC
  AGCAGCCCCTGGGGCGAGAGCTCTCGGAGGCCAACCGTTTTGCCTATGCCGCCCTGTGTGGCATCTCTTT
  GTCACAGCTGTTTCCAGAACCTGAGCAAAGCTCCTTCTGCTCAGAATTTGTGACGGGCCTGGTGAAATGG
  TTGCATCTGTCTGAAACTGTCCTCCCAACCATGATGGCCTTTGCCAGCGGCCTGGGAGGCAAAGGAGATG
  ACATCTTTGCTCAGACTTTATTGAAGGACCCCATTCTGAAAGACAACCCATCAGCCATCAGTCAGGACCT
  CCTGAGCTTCTCACTCAAGGATGGGCACTACGATGCCCGAGCCAGAGTCCTCGTTTGTCATGTGATCTCC
  CTGCTCCAGGTGCCCATGGAGGAGCTTGATATTCTCGAAGAGGTGTTCCTGGAGAGCCTGAAGGACGCCA
  AAGAGGAGGAGTCTGAGACCGCAGAGGAGTCCCGGAAGAGAAAAGAAAAAAGGAGGAAGTGGAAGCGCTA
  TCTCCTGATTGGCCTGGCCACAGTTGGAGGCGGGACAGTGATTGGTGTGACAGGGGGCCTAGCTGCCCCC
  CTTGTTGCCGCCGGAGCTGCGACAATTATAGGCAGCGCTGGGGCAGCTGCGTTGGGCTCCGTGGCTGGCA
  TCGCTGTTATGACCTCACTGTTTGGTGCAGCCGGAGCTGGCCTGACAGGATACAAGATGAAGAAGCGAGT
  CGGGGCCATTGAGGAGTTCATGTTCCTGCCTCTGACGGATGGCAAGCAGCTACATATCACCATCGCCATC
  ACTGGCTGGCTCGGGTCCGGCAGATACCGTACATTCAATGCCCCATGGATGGCCCTAGCCCGCAGCCAGG
  AGCAGTACTGCCTGGCCTGGGAGGCCAAGTACCTGATGGAGTTGGGCAATGCCCTGGAGACCATTCTCAG
  TGGCCTCGCTAACATGGTGGCCCAGGAGGCTCTCAAGTACACCGTGCTCTCTGGTATCGTGGCTGCTCTA
  ACCTTGCCGGCCTCCCTCCTCAGCGTCGCCAATGTCATCGACAACCCCTGGGGGGTGTGTCTCCACCGGT
  CTGCTGAGGTTGGCAAGCACCTAGCACACATCCTTCTCTCGAGGCAACAGGGCCGGAGGCCTGTCACCTT
  GATTGGATTCAGCCTGGGAGCCAGAGTCATCTACTTCTGTCTGCAAGAGATGGCTCAGGAGCAAGATTGC
  CAAGGGATCATCGAGGATGTTGTCCTTCTGGGCGCACCAGTAGAGGGGGACCCCAAGTACTGGGAACCGT
  TCCGGAATGTGGTGTCTGGGAGAATCATCAACGGCTACTGCAGGGGAGACTGGCTGCTGAGTTTCGTGTA
  CCGCACATCTTCTGTGCAGCTCCGCGTCGCAGGCCTCCAGCCTGTGCTGTTGCAGGACCGGAGGATGGAG
  AACGTGGACCTGTCCTCAGTGGTCAATGGCCATTTGGACTATGCCAAGAAAATGGATGTCATCCTGAAGG
  CTGTGGGCATCCGCACCAAGCCTGGCTGGAGTGAGAAGGGACTCCCACTGGCCCCTGGCGGCCTGCCCCA
  AGAAGAGCCTCTCCAGCCAGCCACAGTCTCCACAGATGAGACAATCCACCAGGATGAACAAAAGCAGGGA
  CCTGCCCCAGGAGACAGTCTCAAATCCGCCATTCCTTCCAGTGCCAGCCAAGCCCAGGTGCCAGCAGGGC
  TGGACCAATCCACAGAGGACTCGCTCCCTGCTGCCGCTGCACCAGCAGAGGGGCACCTGGTCTGCAGCCA
  TGGTGTGGGACCCAACCCACTGGGCTGCCCTGACTGCACCCGTGAGACCCAGGAGTCCTGTGCAGAACTA
  GAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210943 representing NM_001034949
  Red=Cloning site Green=Tags(s)
  
  MATWNRPHPRLPVAPEPVAEGESQQPLGRELSEANRFAYAALCGISLSQLFPEPEQSSFCSEFVTGLVKW
  LHLSETVLPTMMAFASGLGGKGDDIFAQTLLKDPILKDNPSAISQDLLSFSLKDGHYDARARVLVCHVIS
  LLQVPMEELDILEEVFLESLKDAKEEESETAEESRKRKEKRRKWKRYLLIGLATVGGGTVIGVTGGLAAP
  LVAAGAATIIGSAGAAALGSVAGIAVMTSLFGAAGAGLTGYKMKKRVGAIEEFMFLPLTDGKQLHITIAI
  TGWLGSGRYRTFNAPWMALARSQEQYCLAWEAKYLMELGNALETILSGLANMVAQEALKYTVLSGIVAAL
  TLPASLLSVANVIDNPWGVCLHRSAEVGKHLAHILLSRQQGRRPVTLIGFSLGARVIYFCLQEMAQEQDC
  QGIIEDVVLLGAPVEGDPKYWEPFRNVVSGRIINGYCRGDWLLSFVYRTSSVQLRVAGLQPVLLQDRRME
  NVDLSSVVNGHLDYAKKMDVILKAVGIRTKPGWSEKGLPLAPGGLPQEEPLQPATVSTDETIHQDEQKQG
  PAPGDSLKSAIPSSASQAQVPAGLDQSTEDSLPAAAAPAEGHLVCSHGVGPNPLGCPDCTRETQESCAEL
  D
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034949
• ORF Size: 1893 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034949.1, NP_001030121.1
• RefSeq Size: 2880 bp
• RefSeq ORF: 1896 bp
• Locus ID: 500573
• UniProt ID: Q499U8
• Cytogenetics: 5q36
• MW: 67.8 kDa