Zdhhc5 (NM_001039338) Rat Tagged ORF Clone

CAT#: RR210777

Zdhhc5 (Myc-DDK-tagged ORF) - Rat zinc finger, DHHC-type containing 5 (Zdhhc5), (10 ug)

Product Images

Circular map for RR210777

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zdhhc5
• Synonyms: DHHC-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210777 representing NM_001039338
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGCAGAGTCTGGAAAGAGATTCAAACCCAGCAAGTATGTACCGGTCTCAGCAGCCGCCATATTTC
  TAGTGGGAGCTACAACACTCTTCTTTGCCTTTACGTGTCCAGGACTAAGCCTGGATGTGTCACCTGCAGT
  GCCCATTTACAATGCGATTATGTTCCTTTTCGTGCTGGCCAACTTCAGCATGGCCACCTTTATGGACCCA
  GGAATTTTCCCCCGAGCTGAAGAAGACGAAGACAAAGAAGATGATTTCCGAGCTCCCCTTTACAAAACAG
  TGGAGATCAAGGGTATCCAGGTGCGAATGAAATGGTGCGCCACCTGCCGCTTTTACCGCCCTCCTAGATG
  CTCCCACTGCAGTGTCTGTGACAACTGTGTGGAGGAATTTGATCATCACTGCCCTTGGGTGAACAACTGT
  ATTGGTCGCAGGAACTACAGATACTTCTTCCTTTTCCTCCTTTCCCTGACAGCCCACATCATGGGTGTGT
  TTGGCTTTGGCCTCCTTTATGTCCTCTGTCACATAGAGGAACTCTCAGGGGTCCGAACTGCTGTCACAAT
  GGCAGTGATGTGTGTGGCTGGCTTGTTTTTCATCCCTGTAGCTGGCCTCACAGGATTTCATGTGGTGCTG
  GTGGCCAGGGGACGCACAACCAATGAACAGGTTACAGGAAAATTCCGGGGAGGTGTGAATCCCTTTACCA
  ATGGCTGTTGTAACAATGTTAGCCGTGTCCTCTGCAGTTCTCCAGCACCCAGGTATTTGGGGAGACCAAA
  GAAAGAGAAGACAATTGTAATCAGACCCCCTTTCCTTCGACCAGAAGTGTCAGATGGGCAGATAACTGTG
  AAGATCATGGATAATGGCATCCAGGGAGAACTGAGGAGGACTAAGTCTAAGGGAAGCCTAGAGATAACAG
  AGAGCCAGTCTGCGGATGCGGAACCTCCACCTCCTCCTAAGCCAGACCTGAGCCGATACACAGGGCTTCG
  AACACACCTCAGCCTGGCTACTAATGAGGATAGCAGTCTCTTGGGCAAAGACAGCCCCCCTACACCTACC
  ATGTACAAGTATCGGCCAGGTTACAGCAGCAGTAGCACATCAGCCGCCATGCCTCATTCCTCCAGCGCCA
  AGTTGAGTCGTGGAGACAGCTTGAAGGAACCAACTTCAATTGCAGATAGCAGCCGCCATCCCAGCTACCG
  CTCAGAACCCAGCTTGGAGCCAGAGAGCTTCCGGTCTCCCACTTTTGGCAAAAGCTTTCATTTTGATCCT
  TTGTCCAGTGGTTCACGATCCTCCAGCCTCAAGTCAGCTCAGGGCACGGGCTTTGAGCTGGGCCAGTTGC
  AGTCCATTCGTTCAGAGGGCACCACCTCCACCTCCTATAAGAGCCTGGCCAATCAGACACGCAATGGAAG
  TCTATCTTATGACAGCCTACTCACTCCTTCAGACAGCCCTGATTTTGAGTCAGTACAGGCAGGGCCTGAG
  CCAGACCCACCTTTAGGCTACACCTCTCCCTTCCTGTCAGCCCGGCTGGCCCAGCAACGGGAAGCCGAGA
  GGCACCCACGTTTGCTGCCAACTGGCCCACCACACCGAGAGCCCTCACCAGTCCGTTATGACAATCTGTC
  TCGCCACATCGTGGCCTCCCTTCAGGAACGAGAGAAGCTGCTACGACAGTCACCTCCACTTGCAGGCCGT
  GAGGAAGAACCAGGCTTGGGAGACTCAGGCATTCAGTCAACACCAGGCTCAGGCCATGCCCCCCGTACTA
  GTTCCTCCTCAGATGATTCCAAGAGATCACCCTTGAGCAAGACTCCACTGGGACGCCCAGCCGTCCCCCG
  TTTTGGCAAACCAGATGGGTTAAGAGGCCGGGGACTAGGGTCCCCTGAACCAGGCACAACTGCCCCCTAC
  CTGGGCCGATCGATGTCCTATAGCAGCCAAAAAGCCCCATCTGGTGTGTCTGAGACAGAGGAAGTAGCAC
  TGCAGCCATTACTGACACCCAAAGACGAAGTACAGCTCAAGACCACCTACAGCAAATCCAATGGGCAGCC
  CAAGAGTATAGGCTCAGCTTCCCCTGGCCCAGGCCAGCCACCTCTCAGTAGCCCCACAAGGGGAGGAGTC
  AAGAAGGTGTCAGGGGTGGGTGGCACCACTTACGAGATTTCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210777 representing NM_001039338
  Red=Cloning site Green=Tags(s)
  
  MPAESGKRFKPSKYVPVSAAAIFLVGATTLFFAFTCPGLSLDVSPAVPIYNAIMFLFVLANFSMATFMDP
  GIFPRAEEDEDKEDDFRAPLYKTVEIKGIQVRMKWCATCRFYRPPRCSHCSVCDNCVEEFDHHCPWVNNC
  IGRRNYRYFFLFLLSLTAHIMGVFGFGLLYVLCHIEELSGVRTAVTMAVMCVAGLFFIPVAGLTGFHVVL
  VARGRTTNEQVTGKFRGGVNPFTNGCCNNVSRVLCSSPAPRYLGRPKKEKTIVIRPPFLRPEVSDGQITV
  KIMDNGIQGELRRTKSKGSLEITESQSADAEPPPPPKPDLSRYTGLRTHLSLATNEDSSLLGKDSPPTPT
  MYKYRPGYSSSSTSAAMPHSSSAKLSRGDSLKEPTSIADSSRHPSYRSEPSLEPESFRSPTFGKSFHFDP
  LSSGSRSSSLKSAQGTGFELGQLQSIRSEGTTSTSYKSLANQTRNGSLSYDSLLTPSDSPDFESVQAGPE
  PDPPLGYTSPFLSARLAQQREAERHPRLLPTGPPHREPSPVRYDNLSRHIVASLQEREKLLRQSPPLAGR
  EEEPGLGDSGIQSTPGSGHAPRTSSSSDDSKRSPLSKTPLGRPAVPRFGKPDGLRGRGLGSPEPGTTAPY
  LGRSMSYSSQKAPSGVSETEEVALQPLLTPKDEVQLKTTYSKSNGQPKSIGSASPGPGQPPLSSPTRGGV
  KKVSGVGGTTYEISV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039338
• ORF Size: 2145 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039338.1, NP_001034427.1
• RefSeq Size: 2697 bp
• RefSeq ORF: 2148 bp
• Locus ID: 362156
• UniProt ID: Q2THW7
• Cytogenetics: 3q24
• MW: 77.4 kDa
• Gene Summary: Palmitoyl acyltransferase for the G-protein coupled receptor SSTR5 and for FLOT2.[UniProtKB/Swiss-Prot Function]