Gucy2d (NM_130737) Rat Tagged ORF Clone

CAT#: RR210558

Gucy2d (Myc-DDK-tagged ORF) - Rat guanylate cyclase 2d (Gucy2d), (10 ug)

Product Images

Circular map for RR210558

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gucy2d
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210558 representing NM_130737
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGTCTGCAGCAGGGCTGTCACCCCGAAGGCCAGGACTGGACAGCGCCACACTGGAAGACCTGCC
  GGGCCCTGCCAGGGCCACGGGGACTCACGGTCAGACATCTGAGGACAGTCTCTTCCATATCGGTCTTCTC
  TGTGGTATTTTGGGGTGTCTTGCTGTGGGCTGACTCTCTTTCCCTGCCAGCATGGGCAAGAGAGACCTTC
  ACCCTTGGGGTGCTGGGTCCCTGGGACTGTGACCCTATCTTTGCCCAGGCACTCCCTAGCATGGCTACCC
  AGTTGGCTGTAGATCGAGTCAATCAGGACGCCTCACTGCTGCTGGGCTCACAGTTGGATTTCAAGATCCT
  CCCTACAGGCTGTGACACCCCTCATGCCCTGGCCACATTTGTGGCCCACAGGAACACAGTGGCTGCTTTT
  ATAGGCCCTGTCAATCCTGGGTATTGTCCAGCAGCAGCTCTGCTGGCCCAAGGCTGGGGCAAGTCCCTCT
  TCTCCTGGGCCTGTGGAGCTCCAGAGGGAGGAGGTGCCTTAGTGCCCACTTTGCCTTCTATGGCCGACGT
  GCTACTGTCTGTCATGAGACACTTTGGCTGGGCTCGCTTGGCCATCGTGTCCTCTCACCAGGACATCTGG
  GTAACCACAGCCCAGCAGCTAGCCACAGCTTTCAGGGCCCATGGGCTGCCTATCGGACTGATCACCTCCT
  TGGGACCTGGAGAGAAGGGGGCCACGGAGGTTTGCAAGCAGCTCCACAGTGTGCATGGTCTGAAAATCGT
  GGTTCTGTGCATGCACTCAGCGCTGCTTGGAGGCCTAGAGCAGACAGTCCTGCTGCGCTGCGCGAGGGAG
  GAGGGCCTGACAGATGGCAGACTGGTCTTCTTGCCCTACGACACGCTGCTCTTTGCCCTGCCCTATCGTA
  ACCGCTCTTACCTGGTCCTGGATGATGATGGGCCCCTTCAGGAGGCCTACGATGCAGTACTCACCATCAG
  TCTGGACACCAGTCCTGAGAGCCATGCCTTCACTGCTACCAAGATGAGAGGAGGGGCAGCTGCCAACTTG
  GGGCCCGAGCAGGTGTCCCCACTCTTTGGAACTATCTACGATGCCGTCATCCTGTTGGCCCATGCCCTGA
  ACCACTCTGAGACCCATGGAACGGGGCTCTCAGGGGCTCACTTAGGGAACCATATAAGAGCTCTTGATGT
  GGCTGGTTTTAGCCAGAGAATCCGGATAGATGGGAAAGGCAGAAGGCTACCCCAATACGTCATCCTGGAC
  ACAAATGGTGAAGGAAGCCAGTTGGTTCCCACCCACATCCTGGACGTGAGCACACAGCAGGTGCAGCCCC
  TGGGAACGGCGGTACACTTCCCTGGAGGGAGCCCTCCAGCCCATGATGCCAGCTGCTGGTTCGACCCCAA
  CACACTATGCATACGAGGTGTGCAGCCCCTGGGCAGCCTCCTGACTCTGACGATAACCTGTGTCCTGGCT
  CTGGTTGGTGGATTCCTTGCTTACTTTATCAGGTTAGGCCTCCAGCAGCTGCGGCTGCTGCGGGGCCCCC
  ATCGGATCCTGCTGACACCTCAAGAGCTCACCTTCCTCCAGCGAACCCCCAGCCGGCGGAGGCCACATGT
  GGACAGTGGCAGCGAGTCAAGAAGTGTGGTAGATGGTGGGAGTCCACAGTCAGTGATCCAGGGGTCAACA
  AGGAGCGTACCAGCCTTCCTGGAGCACACCAACGTGGCCCTGTACCAGGGAGAGTGGGTGTGGCTGAAGA
  AGTTTGAGGCAGGCACGGCTCCTGATCTGCGGCCCAGCAGCCTCAGCCTCCTGAGAAAGATGCGGGAGAT
  GCGGCATGAGAATGTCACCGCCTTCCTGGGTCTCTTTGTGGGCCCTGAGGTTAGTGCGATGGTGCTGGAG
  CACTGTGCCCGTGGCAGCCTGGAGGACCTGCTACGGAATGAGGACCTGAGGCTAGACTGGACCTTCAAGG
  CCTCCTTACTGTTGGATCTGATCCGAGGTTTGCGGTATCTGCACCATCGACATTTCCCCCATGGGCGCCT
  CAAGTCCAGGAACTGTGTGGTGGACACTCGCTTTGTGCTCAAGATCACTGACCATGGTTATGCAGAGTTC
  CTGGAGTCTCACTGCTCTTTCAGGCCCCAGCCGGCCCCAGAAGAGCTTCTGTGGACGGCTCCTGAGCTGT
  TGCGGGGGCCTCGGGGGCCTTGGGGGCCTGGGAAGGCCACCTTCAAAGGTGATGTTTTCAGCCTGGGCAT
  CATCCTGCAGGAGGTACTGACCCGAGACCCACCCTACTGCTCCTGGGGACTCTCAGCAGAAGAGATCATC
  CGGAAGGTGGCATCTCCCCCTCCTCTGTGCCGGCCTCTGGTGTCCCCTGACCAGGGTCCCCTGGAGTGCA
  TCCAGTTGATGCAGCTGTGCTGGGAGGAAGCTCCAGATGACAGGCCAAGCTTGGACCAGATCTACACACA
  GTTCAAAAGCATCAACCAAGGCAAGAAGACAAGTGTTGCTGACTCCATGCTGCGGATGCTGGAGAAGTAT
  TCCCAAAGCCTGGAAGGTCTGGTCCAGGAGCGGACTGAGGAGCTGGAACTGGAGAGGCGGAAGACAGAGA
  GGCTGCTCTCACAGATGCTCCCCCCGTCTGTCGCCCATGCTCTAAAGATGGGAACAACTGTGGAGCCCGA
  GTACTTTGACCAAGTCACCATCTATTTCAGTGACATCGTGGGCTTTACCACCATCTCAGCCTTGAGTGAG
  CCCATTGAGGTGGTGGGCTTCCTCAATGATCTCTACACGATGTTTGATGCTGTTCTAGACAGCCATGATG
  TGTATAAGGTAGAAACCATAGGGGATGCCTACATGGTGGCATCTGGGCTCCCTCGGCGCAACGGAAATCG
  GCATGCTGCTGAGATTGCCAACATGGCTCTGGAGATCCTTAGCTACGCAGGCAACTTCCGGATGAGGCAT
  GCACCTGATGTGCCCATCCGTGTCAGGGCCGGTCTGCATTCAGGGCCCTGTGTGGCAGGTGTTGTGGGTC
  TCACCATGCCTCGGTACTGCCTCTTTGGGGACACCGTGAACACTGCTTCCAGGATGGAATCCACTGGACT
  GCCATACAGAATCCACGTCAGCCGAAACACTGTCCAGGCCCTGCTTAGCCTTGACGAAGGCTACAAAATT
  GATGTCAGAGGTCAGACCGAACTGAAGGGGAAGGGCTTGGAGGAGACTTACTGGCTGACAGGGAAGACAG
  GATTTTGCAGATCCCTCCCTACACCTCTGTCCATCCAGCCTGGAGACCCATGGCAGGACCATATAAACCA
  AGAAATCCGGACTGGCTTTGCAAAGCTCGCCAGAGTCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210558 representing NM_130737
  Red=Cloning site Green=Tags(s)
  
  MAGLQQGCHPEGQDWTAPHWKTCRALPGPRGLTVRHLRTVSSISVFSVVFWGVLLWADSLSLPAWARETF
  TLGVLGPWDCDPIFAQALPSMATQLAVDRVNQDASLLLGSQLDFKILPTGCDTPHALATFVAHRNTVAAF
  IGPVNPGYCPAAALLAQGWGKSLFSWACGAPEGGGALVPTLPSMADVLLSVMRHFGWARLAIVSSHQDIW
  VTTAQQLATAFRAHGLPIGLITSLGPGEKGATEVCKQLHSVHGLKIVVLCMHSALLGGLEQTVLLRCARE
  EGLTDGRLVFLPYDTLLFALPYRNRSYLVLDDDGPLQEAYDAVLTISLDTSPESHAFTATKMRGGAAANL
  GPEQVSPLFGTIYDAVILLAHALNHSETHGTGLSGAHLGNHIRALDVAGFSQRIRIDGKGRRLPQYVILD
  TNGEGSQLVPTHILDVSTQQVQPLGTAVHFPGGSPPAHDASCWFDPNTLCIRGVQPLGSLLTLTITCVLA
  LVGGFLAYFIRLGLQQLRLLRGPHRILLTPQELTFLQRTPSRRRPHVDSGSESRSVVDGGSPQSVIQGST
  RSVPAFLEHTNVALYQGEWVWLKKFEAGTAPDLRPSSLSLLRKMREMRHENVTAFLGLFVGPEVSAMVLE
  HCARGSLEDLLRNEDLRLDWTFKASLLLDLIRGLRYLHHRHFPHGRLKSRNCVVDTRFVLKITDHGYAEF
  LESHCSFRPQPAPEELLWTAPELLRGPRGPWGPGKATFKGDVFSLGIILQEVLTRDPPYCSWGLSAEEII
  RKVASPPPLCRPLVSPDQGPLECIQLMQLCWEEAPDDRPSLDQIYTQFKSINQGKKTSVADSMLRMLEKY
  SQSLEGLVQERTEELELERRKTERLLSQMLPPSVAHALKMGTTVEPEYFDQVTIYFSDIVGFTTISALSE
  PIEVVGFLNDLYTMFDAVLDSHDVYKVETIGDAYMVASGLPRRNGNRHAAEIANMALEILSYAGNFRMRH
  APDVPIRVRAGLHSGPCVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVSRNTVQALLSLDEGYKI
  DVRGQTELKGKGLEETYWLTGKTGFCRSLPTPLSIQPGDPWQDHINQEIRTGFAKLARVC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130737
• ORF Size: 3330 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130737.4, NP_570093.1
• RefSeq Size: 3645 bp
• RefSeq ORF: 3333 bp
• Locus ID: 113911
• UniProt ID: P51839
• Cytogenetics: 1q32
• MW: 122 kDa
• Gene Summary: member of the membrane receptor guanyly cyclase family, specifically expressed in a subpopulation of olfactory sensory neurons; may function directly in odor recognition [RGD, Feb 2006]