Farp1 (NM_001107287) Rat Tagged ORF Clone

CAT#: RR210537

Farp1 (Myc-DDK-tagged ORF) - Rat FERM, RhoGEF (Arhgef) and pleckstrin domain protein 1 (chondrocyte-derived) (Farp1), (10 ug)

Product Images

Circular map for RR210537

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Farp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210537 representing NM_001107287
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGAAATAGAGCAGAAGCCCACCCCAGCATCACGTCTGGGGGCCCCAGAGAACTCGGGGATCAGCA
  CCTTGGAACGAGGACAGAAACCACCGCCCACACCTTCAGGAAAACTTATGACTGTCAAAATCCAGATGCT
  GGACGACACCCAGGAAGCCTTTGAAGTTCCACAACGGGCACCAGGGAAGGTCCTCTTTGATGCTGTGTGC
  AACCACCTCAACCTTGTGGAGGGTGACTACTTCGGCCTGGAGTTCCCTGACCACAGAAAGATCGTGGTGT
  GGCTGGATCTTCTGAAGCCCATTGTGAAGCAGATTCGAAGGCCGAAGCACGTGCTTGTTAAGTTTGTTGT
  GAAATTCTTCCCGCCCGACCACACCCAACTCCAGGAAGAACTCACCAGGTACCTGTTCGCCCTGCAGGTG
  AAGCAGGACTTGGCTCAAGGCAGGCTGACGTGTAACGACACCAGCGCGGCTCTCTTGATCTCGCACATCG
  TACAATCTGAGATTGGGGATTTCGACGAAGCTTTGGACAGAGAACACTTAGCAAAAAATAAGTATGTACC
  TCAACAGGATGCACTAGAAGACAAAATCGTGGAGTTCCACCACAGCCACATTGGACAAACCCCGGCAGAG
  TCAGATTTCCAGCTCCTGGAGGTGGCCCGTCGACTGGAGATGTATGGAATCCGGTTACACCCTGCCAAGG
  ACAGGGAAGGCACCAAGATCAACCTTGCTGTCGCCAACACTGGAATTCTAGTGTTTCAGGGCTTCACGAA
  GATCAATGCTTTTAACTGGGCCAAGGTGCGGAAGCTGAGCTTCAAGCGGAAGCGTTTCCTCATCAAGCTC
  AGACCTGACGTGAACAGCTCGTACCAGGACACCTTGGAGTTCCTGATGGCCGGTCGAGACTTCTGCAAGT
  CCTTCTGGAAGATATGTGTGGAACACCATGCCTTCTTCAGACTCTTTGAAGAACCCAAGCCAAAGCCAAA
  GCCAGTTCTCTTCAGCCGAGGGTCGTCCTTCCGGTTTAGTGGTCGGACTCAGAAGCAAGTTCTTGACTAC
  GTTAAAGAAGGAGGCCACAAGAAAGTGCAGTTTGAAAGAAAACACAGCAAGATCCATTCCACTAGGAGCC
  TTGTCTCACAGCCCACAGCACCGAATTCGGAAGTGCCAAAACAGTCTCCGCAGAGCGCCAGCCTTACGTT
  CGGTGAAGGTACCGAGTCTCCCAGTGCCCAGAGCTGCCAACAAGCCAAGGAAACGAAAGTTTGTACCTTG
  GAGCCTGGACCGCGCCAAAGTCCCGCCCTCTCTAAGAGCCCTTCAGGGAGCAAAGCTGCGGATGGAACCG
  CTGCTGCGGCACCGCCCGAGGAAGAGGATGAGGAAGAGGGTGGCAAGGATGGCATCCGGCCAAGTAACCC
  TCAGCCCCCGCAGCCAAGCACAGGCTCCCTGACTGGTAGCCCTCACCTTTCAGAGCTGTCCATCAACTCG
  CAGGGAGGAGCCGCCCCAGCCAACGTGACCCTGTCTCCCAACCTGAGTCCTGACAACAAGCAGGCCTCTC
  CCCTGATCAGCCCGCTCCTGAACGACCAAGCGTGCCCAAGGACCGACGATGAAGAAGAGGGCCGGCGAAA
  GAGATTCCCAACCGACAAGGCATACTACATTGCTAAGGAAGTCTCCACCACAGAACGCACATACCTGAAG
  GATCTTGAGGTCATCGCTTCGTGGTTCCAGAGCACAGTCAGCAAAGAGGACTCCATGCCTGAAGCTTTGA
  AAAGTCTCATATTTCCAAACTTCGAACCTTTGCACAAGTTCCACACCAATTTTCTGAAGGAGATTGAGCA
  GCGTCTCGCCCTGTGGGAAGGCCGCTCAAACGCCCACATCAGAGGAGATTACCAAAGAATCGGGGACGTC
  ATGCTGAAGAACATTCAGGGCATGAAGCACCTGGCGGCCCACCTCTGGAAGCACAGCGAGGCCTTGGAGG
  CCCTGGAGACCTCCATCAAGGGCTCCCGGCGGCTGGAACACTTCTGCCGAGACTTCGAGTTGCAGAAGGT
  GTGTTACCTGCCGCTCAACACCTTCCTCCTGCGCCCGCTGCACCGGCTCATGCACTACAAGCAGGTCCTG
  GAGAGGCTGTGCAAGCATCACCCTCCGAACCATGCCGACTTCAGGGACTGCAGAGCTGCGCTGGCAGAGA
  TCACAGAGATGGTGGCACAGCTGCACGGTACCATGATCAAGATGGAGAACTTCCAGAAGCTGCATGAGCT
  CAAGAAGGATCTGATTGGCATTGACAATCTTGTGATCCCAGGAAGGGAGTTCATCCGCCTGGGTAGCCTC
  AGCAAGCTCTCCGGGAAGGGGCTTCAACAGCGCATGTTCTTCCTGTTCAACGATGTCTTGCTGTATACCA
  GCCGGGGACTGACGGCATCTAATCAGTTTAAAGTCCACGGACAGCTCCCGCTCTATGGCATGACGATCGA
  GGAGAGTGAGGAGGAGTGGGGCGTACCCCACTGCCTGACCCTGCGGGGCCAGCGGCAGTCCATCATCGTG
  GCCGCCAGTTCCCGATCAGAGATGGAAAAATGGCTGGAGGACATCCAGATGGCCATTGACCTGGCTGAGA
  AAAGCAATGGGCCCACCCCCGAACTCCTCGCCAGCAGCCCCCCTGACAACAAGTCCCCGGATGAAGCCAC
  AGCTGCTGACCAGGAGTCAGAAGACGACCTGAGCGCCTCTCGGACCTCGCTGGAGCGCCAGGCCCCTCAC
  CGTGGCAACACCATGGTGCACGTGTGCTGGCATCGCAGCACCAGCGTCTCCATGGTGGACTTCAGCATTG
  CCGTGGAGAACCAGCTCTCTGGGAACTTGCTGAGAAAGTTCAAGAACAGCAATGGCTGGCAGAAGCTGTG
  GGTGGTATTCACCAACTTCTGCCTGTTCTTCTACAAATCACACCAGGACAGTCACCCCCTCGCCAGTCTA
  CCGCTGTTGGGCTACTCACTCACCATTCCCTCAGAGTCTGAGAACATCCACAAGGACTACGTGTTCAAGC
  TGCACTTCAAGTCCCACGTGTACTACTTCAGGGCTGAAAGCGAATACACATTTGAAAGGTGGATGGAAGT
  GATCCGAAGTGCCACCAGCTCGGCCTCTCGGGCCCACAGCCTGAGTCACAAAGAATCTCACCTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210537 representing NM_001107287
  Red=Cloning site Green=Tags(s)
  
  MGEIEQKPTPASRLGAPENSGISTLERGQKPPPTPSGKLMTVKIQMLDDTQEAFEVPQRAPGKVLFDAVC
  NHLNLVEGDYFGLEFPDHRKIVVWLDLLKPIVKQIRRPKHVLVKFVVKFFPPDHTQLQEELTRYLFALQV
  KQDLAQGRLTCNDTSAALLISHIVQSEIGDFDEALDREHLAKNKYVPQQDALEDKIVEFHHSHIGQTPAE
  SDFQLLEVARRLEMYGIRLHPAKDREGTKINLAVANTGILVFQGFTKINAFNWAKVRKLSFKRKRFLIKL
  RPDVNSSYQDTLEFLMAGRDFCKSFWKICVEHHAFFRLFEEPKPKPKPVLFSRGSSFRFSGRTQKQVLDY
  VKEGGHKKVQFERKHSKIHSTRSLVSQPTAPNSEVPKQSPQSASLTFGEGTESPSAQSCQQAKETKVCTL
  EPGPRQSPALSKSPSGSKAADGTAAAAPPEEEDEEEGGKDGIRPSNPQPPQPSTGSLTGSPHLSELSINS
  QGGAAPANVTLSPNLSPDNKQASPLISPLLNDQACPRTDDEEEGRRKRFPTDKAYYIAKEVSTTERTYLK
  DLEVIASWFQSTVSKEDSMPEALKSLIFPNFEPLHKFHTNFLKEIEQRLALWEGRSNAHIRGDYQRIGDV
  MLKNIQGMKHLAAHLWKHSEALEALETSIKGSRRLEHFCRDFELQKVCYLPLNTFLLRPLHRLMHYKQVL
  ERLCKHHPPNHADFRDCRAALAEITEMVAQLHGTMIKMENFQKLHELKKDLIGIDNLVIPGREFIRLGSL
  SKLSGKGLQQRMFFLFNDVLLYTSRGLTASNQFKVHGQLPLYGMTIEESEEEWGVPHCLTLRGQRQSIIV
  AASSRSEMEKWLEDIQMAIDLAEKSNGPTPELLASSPPDNKSPDEATAADQESEDDLSASRTSLERQAPH
  RGNTMVHVCWHRSTSVSMVDFSIAVENQLSGNLLRKFKNSNGWQKLWVVFTNFCLFFYKSHQDSHPLASL
  PLLGYSLTIPSESENIHKDYVFKLHFKSHVYYFRAESEYTFERWMEVIRSATSSASRAHSLSHKESHLY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107287
• ORF Size: 3147 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107287.1, NP_001100757.1
• RefSeq Size: 4564 bp
• RefSeq ORF: 3150 bp
• Locus ID: 306183
• UniProt ID: F1LYQ8
• Cytogenetics: 15q24
• MW: 118.8 kDa
• Gene Summary: May play a role in semaphorin signaling (By similarity). Functions as guanine nucleotide exchange factor for RAC1. Plays a role in the assembly and disassembly of dendritic filopodia, the formation of dendritic spines, regulation of dendrite length and ultimately the formation of synapses.[UniProtKB/Swiss-Prot Function]