Nhlrc2 (NM_001107444) Rat Tagged ORF Clone

CAT#: RR210188

Nhlrc2 (Myc-DDK-tagged ORF) - Rat NHL repeat containing 2 (Nhlrc2), (10 ug)

Product Images

Circular map for RR210188

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nhlrc2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210188 representing NM_001107444
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTCCCCGGAGCGCGCGGCGCGAGTCTCTCGGGTCTTCTGCCCGCGCAGACCTCTCTAGAGTACG
  CCCTGCTGGACGCCGTCACCCAGCAGGAGAAGGATGACCTGGTCTATCAGTATCTGCAGAAGGTGGACGG
  CTGGGAGCAGGACCTAGCGGTGCCTGAGTTTCCGGAAGGACTAGAATGGCTGAATACAGAAGAGCCTTTG
  TCGATTTACAGAGACCTGTGTGGGAAAGTGGTCATCCTCGACTTCTTCACTTACTGCTGCATCAACTGCA
  TCCATGTGCTGCCCGATCTCCACGCCCTGGAGCGCACGTTCTCTGACACAGATGGCCTTCTGATTGTCGG
  TGTTCACTCAGCTAAGTTTCCAAATGAAAAGGTCCTGGACAACATCAAGAGTGCTGTCCTTCGGTACAAC
  ATCACCCACCCTGTGGTTAACGACACAGATGCTAGCCTTTGGCAGGAGCTTGAAGTCTCCTGCTGGCCAA
  CACTGGTCATACTGGGACCCCGTGGAAACCTGCTGTTTTCTTTGATTGGAGAGGGTCACAAAGATAAACT
  GTTTTCATATACATCTATTGCTTTAAAGTATTACAAAGACAGAGGACAGATCAGAGATGGAAAAATTGGG
  ATAAAACTCTTTAAAGAGTCTTTGCCGCCTTCACCATTGCTGTTCCCTGGCAAAGTAGCCGCAGACCCTA
  CTACTGGTCGGCTGGTGGTAGCAGACACCGGGCACCACAGGATCTTGGTTCTTCAGAAGAACGGGCAGAT
  TCAGTACAGTATTGGAGGACCTAATCCTGGAAGGAAAGATGGGGTGTTTTCAGAGTCGTCTTTTCATTCT
  CCACAGGGTGTAGCTATCGCAGATAATGTCATCTATGTGGCCGACACTGAAAACCACCTTATAAGAAAGA
  TAGACTTAGAAGCTGAGAAGGTGAGCACTGTAGCTGGCATCGGTGTTCAAGGGACAGATACAGAAGGCGG
  TGAGGAGGGAGACAAACAGCCCATCAGCTCCCCGTGGGACGTTGCACTTGGGACCTCAGGTTCAAAGGTC
  CAAAGAAATGACATCCTGTGGATAGCCATGGCAGGGACTCATCAGATATGGGCCTTCCTCCTGGACTCTG
  GCACACTGCCAAAGAAAAGTGACCTAAAGAAAGGAACCTGCCTCCGTTTTGCTGGAAGTGGAAATGAAGA
  GAACCGAAACAACGCATATCCTCACAAGGCGGGCTTCGCCCAGCCATCAGGCCTCGCTCTCGCTCCAGAA
  GAGCCCTGGAGCTGCCTCTTTGTGGCTGACAGCGAGAGCAGCACTGTGAGGACTGTCTCGCTGAGAGACG
  GAGCCGTGAAACACCTCGTTGGCGGAGAGCGAGACCCCATGAATTTATTTGCTTTTGGTGATGTTGATGG
  AGCAGGCATCAGTGCGAAGCTTCAGCACCCACTTGGGGTTGCATGGGATGAGAAGAGGCATCTGCTCTAT
  GTGGCCGACTCCTACAATCACAAGATTAAAGTTGTGGATCCAAACACAAAAGTCTGTACAACGTTAGCAG
  GCACTGGAGATGCGAATGACGCCAGTTCCAGTTTTGCGGAGTCGGCCTTTAATGAGCCGGGAGGCTTGTG
  TATCGGTGAGCGTGGTCGGCTGCTCTATGTAGCAGACACTAATAATCACCAGATTAAAGTGATGGATTTA
  GAAGCAAGAACAGTTTCTGTGCTCCCTGTCTTCAAGTCTGACAGTGCTGTGGTAGACGGCTCCTTCCCTA
  GAGAGAAACAGAAGACAGTGCCCAAAGTGCCGAAGTCCGCCCCAAATATCAGCCTCCCCCCTGTGACAGT
  GCATCCGGGCCAGGCTCTGCAGCTCGGGCTCCGGCTAGAGCTTCCGCCAGGAGCAAAGCTGACAGAAGGT
  GCCCCCAGCGGCTGGTTCCTGAAAGCTGAAGGCAATGAATGGCTTCTTCAGGAACAGATCCCATCTGGAG
  ACATAGAGAATATTTCCAATCAGCCGGCAATCTCCCTGCAGATCCCTACTGAGTGCTTGTCCCTGGAGGC
  CGTGGTGTCTGTCACCGTGTTTCTTTATTACTGCAGTGCTGACAGCAGTGCATGTATGATGAAGGGAGTT
  GTGTTCAGGCAGCCTTTGCAGATAAACAGTGTCCAGCCAGCGTGTGCAGCTCCTGTGGAGCTCACGTACG
  CATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210188 representing NM_001107444
  Red=Cloning site Green=Tags(s)
  
  MAVPGARGASLSGLLPAQTSLEYALLDAVTQQEKDDLVYQYLQKVDGWEQDLAVPEFPEGLEWLNTEEPL
  SIYRDLCGKVVILDFFTYCCINCIHVLPDLHALERTFSDTDGLLIVGVHSAKFPNEKVLDNIKSAVLRYN
  ITHPVVNDTDASLWQELEVSCWPTLVILGPRGNLLFSLIGEGHKDKLFSYTSIALKYYKDRGQIRDGKIG
  IKLFKESLPPSPLLFPGKVAADPTTGRLVVADTGHHRILVLQKNGQIQYSIGGPNPGRKDGVFSESSFHS
  PQGVAIADNVIYVADTENHLIRKIDLEAEKVSTVAGIGVQGTDTEGGEEGDKQPISSPWDVALGTSGSKV
  QRNDILWIAMAGTHQIWAFLLDSGTLPKKSDLKKGTCLRFAGSGNEENRNNAYPHKAGFAQPSGLALAPE
  EPWSCLFVADSESSTVRTVSLRDGAVKHLVGGERDPMNLFAFGDVDGAGISAKLQHPLGVAWDEKRHLLY
  VADSYNHKIKVVDPNTKVCTTLAGTGDANDASSSFAESAFNEPGGLCIGERGRLLYVADTNNHQIKVMDL
  EARTVSVLPVFKSDSAVVDGSFPREKQKTVPKVPKSAPNISLPPVTVHPGQALQLGLRLELPPGAKLTEG
  APSGWFLKAEGNEWLLQEQIPSGDIENISNQPAISLQIPTECLSLEAVVSVTVFLYYCSADSSACMMKGV
  VFRQPLQINSVQPACAAPVELTYAF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107444
• ORF Size: 2175 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107444.1, NP_001100914.1
• RefSeq Size: 4565 bp
• RefSeq ORF: 2178 bp
• Locus ID: 307986
• Cytogenetics: 1q55
• MW: 78.6 kDa