Hmgcs2 (NM_173094) Rat Tagged ORF Clone

CAT#: RR210173

Hmgcs2 (Myc-DDK-tagged ORF) - Rat 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) (Hmgcs2), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR210173

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hmgcs2
• Synonyms: Hmgcs1; Mt3h3mg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210173 representing NM_173094
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGGCTTTTGGCTCCAGCAAGGCGGGTCCTGCAAGTGAAGAGAGTGATGCAGGAATCTTCGCTCT
  CACCCGCTCACCTGCTCCCCGCAGCCCAGCAGAGGTTTTCTACAATCCCTCCTGCTCCCCTGGCCAAAAC
  TGATACATGGCCAAAAGATGTGGGCATCCTTGCCCTGGAGGTCTACTTTCCAGCCCAATATGTGGACCAA
  ACTGACCTGGAGAAGTTCAACAATGTGGAAGCAGGGAAGTACACAGTGGGCTTGGGCCAGACCCGTATGG
  GCTTCTGTTCGGTCCAGGAGGACATCAACTCCCTGTGCCTCACAGTGGTGCAGAGGCTGATGGAACGCAC
  AAAGCTGCCATGGGATGCCGTAGGCCGCCTGGAAGTGGGCACGGAAACCATCATTGACAAGTCCAAGGCT
  GTCAAGACAGTGCTCATGGAGCTCTTCCAGGATTCAGGCAACACTGACATCGAGGGCATAGATACCACCA
  ACGCCTGCTATGGTGGCACTGCCTCCCTCTTCAACGCTGCCAACTGGATGGAGTCCAGCTACTGGGATGG
  TCGCTATGCCCTGGTGGTCTGTGGTGATATCGCAGTCTACCCAAGTGGTAACGCCCGCCCCACAGGTGGT
  GCCGGGGCTGTGGCAATGCTGATTGGGCCCAAGGCCCCGCTAGTCCTGGAACAAGGGCTGAGGGGAACCC
  ACATGGAGAACGCCTATGACTTCTACAAACCAAACTTGGCCTCAGAGTATCCACTGGTGGATGGGAAGCT
  GTCTATCCAGTGCTACCTGCGGGCCTTGGACCGATGCTATGCAGCTTACCGCAGGAAAATCCAGAATCAG
  TGGAAGCAAGCTGGAAACAACCAGCCTTTCACCCTCGATGACGTGCAATATATGATCTTCCACACACCCT
  TTTGCAAGATGGTCCAGAAATCCCTAGCTCGGCTGATGTTCAATGACTTCCTGTCATCTAGCAGTGACAA
  GCAGAACAACTTATACAAGGGTCTAGAGGCCTTCAAGGGTCTAAAGCTGGAAGAAACCTACACCAACAAG
  GATGTTGACAAGGCTCTGCTGAAGGCCTCCCTGGACATGTTCAACAAGAAAACCAAGGCCTCCCTTTACC
  TCTCCACAAACAATGGGAACATGTACACCTCGTCCCTCTACGGGTGCCTGGCCTCACTTCTCTCCCACCA
  CTCTGCCCAAGAATTGGCCGGCTCCAGGATTGGAGCATTCTCCTACGGCTCAGGCTTAGCAGCAAGTTTC
  TTCTCATTTCGAGTGTCCAAGGACGCTTCCCCAGGTTCCCCTCTGGAGAAGCTGGTGTCTAGTGTGTCAG
  ATCTGCCCAAACGTCTAGACTCCCGGAGACGCATGTCCCCTGAGGAATTCACAGAAATAATGAATCAGAG
  AGAGCAATTTTACCACAAGGTGAACTTCTCTCCCCCTGGTGACACAAGCAACCTCTTCCCAGGCACTTGG
  TACCTTGAACGAGTGGATGAGATGCACCGCAGAAAATATGCCCGGCGTCCCGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210173 representing NM_173094
  Red=Cloning site Green=Tags(s)
  
  MQRLLAPARRVLQVKRVMQESSLSPAHLLPAAQQRFSTIPPAPLAKTDTWPKDVGILALEVYFPAQYVDQ
  TDLEKFNNVEAGKYTVGLGQTRMGFCSVQEDINSLCLTVVQRLMERTKLPWDAVGRLEVGTETIIDKSKA
  VKTVLMELFQDSGNTDIEGIDTTNACYGGTASLFNAANWMESSYWDGRYALVVCGDIAVYPSGNARPTGG
  AGAVAMLIGPKAPLVLEQGLRGTHMENAYDFYKPNLASEYPLVDGKLSIQCYLRALDRCYAAYRRKIQNQ
  WKQAGNNQPFTLDDVQYMIFHTPFCKMVQKSLARLMFNDFLSSSSDKQNNLYKGLEAFKGLKLEETYTNK
  DVDKALLKASLDMFNKKTKASLYLSTNNGNMYTSSLYGCLASLLSHHSAQELAGSRIGAFSYGSGLAASF
  FSFRVSKDASPGSPLEKLVSSVSDLPKRLDSRRRMSPEEFTEIMNQREQFYHKVNFSPPGDTSNLFPGTW
  YLERVDEMHRRKYARRPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173094
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173094.2, NP_775117.2
• RefSeq Size: 2007 bp
• RefSeq ORF: 1527 bp
• Locus ID: 24450
• UniProt ID: Q68G44
• Cytogenetics: 2q34
• MW: 56.9 kDa
• Gene Summary: HMG-CoA cycle enzyme that catalyzes a step in ketone-body biosynthesis [RGD, Feb 2006]