Cry2 (NM_133405) Rat Tagged ORF Clone

CAT#: RR210144

Cry2 (Myc-DDK-tagged ORF) - Rat cryptochrome 2 (photolyase-like) (Cry2), (10 ug)

Product Images

Circular map for RR210144

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cry2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210144 representing NM_133405
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCTGCTGTGGTGGCAGCGACGGTCCCCGCGCAGTCGATGGGCGCGGACGGCGCGTCCTCCG
  TGCACTGGTTCCGCAAAGGACTACGGCTCCACGACAACCCCGCGCTGTTAGCTGCCGTGCGCGGGGCGCG
  CTGTGTGCGCTGCGTCTACATCCTCGACCCGTGGTTCGCGGCCTCCTCGTCAGTGGGCATCAACCGATGG
  AGGTTCCTACTGCAGTCTCTAGAAGATCTGGACACAAGCTTAAGAAAGCTGAATTCCCGTCTGTTTGTAG
  TCCGGGGTCAGCCAGCTGATGTGTTCCCAAGGCTTTTCAAGGAATGGGGGGTGACCCGCTTGACCTTTGA
  ATATGACTCCGAACCCTTTGGGAAAGAACGGGATGCAGCCATTATGAAGATGGCCAAGGAGGCGGGTGTG
  GAGGTGGTGACTGAGAACTCTCACACCCTTTATGACTTAGACAGAATCATCGAACTGAATGGGCAGAAAC
  CACCCCTTACCTACAAGCGCTTTCAGGCTCTCATCAGCCGTATGGAGCTGCCCAAGAAGCCAGTGGGGGC
  TGTGAGCAGCCAGCATATGGAGAACTGCAGAGCTGAGATCCAGGAGAACCATGATGACACCTATGGCGTG
  CCTTCCTTAGAGGAACTGGGATTCCCCACAGAAGGACTTGGCCCAGCTGTTTGGCAAGGAGGAGAGACAG
  AAGCTCTGGCCCGCCTGGATAAGCACTTGGAACGGAAGGCCTGGGTTGCCAACTATGAGAGACCTCGGAT
  GAATGCCAATTCCTTGCTGGCCAGCCCCACAGGCCTCAGCCCCTACCTGCGCTTTGGCTGCCTCTCCTGC
  CGCCTCTTCTACTACCGCCTGTGGGACTTGTACAGAAAGGTGAAGAGGAACAGCACACCCCCCCTCTCCT
  TATTTGGACAACTCCTATGGCGAGAATTCTTCTATACAGCGGCCACCAACAACCCCAGGTTTGACCGAAT
  GGAGGGGAACCCCATCTGCATCCAGATCCCCTGGGACCGCAACCCCGAAGCCCTGGCCAAGTGGGCCGAG
  GGCAAGACAGGCTTCCCTTGGATTGACGCCATCATGACCCAACTGAGGCAGGAGGGCTGGATCCACCACC
  TGGCCCGGCACGCTGTGGCCTGCTTCCTCACCCGAGGGGACCTCTGGGTCAGCTGGGAGAGCGGGGTCCG
  GGTATTTGATGAGTTGCTCCTGGATGCAGATTTCAGCGTGAATGCAGGCAGCTGGATGTGGCTGTCCTGC
  AGTGCTTTCTTCCAACAGTTCTTCCACTGCTACTGCCCTGTGGGCTTTGGCCGACGCACGGACCCCAGTG
  GGGACTACATCCGGCGATACCTGCCCAAACTGAAAGGCTTCCCCTCTCGATATATCTATGAGCCCTGGAA
  TGCTCCCGAGTCGGTTCAGAAGGCCGCTAAGTGCATCATTGGCGTGGACTACCCACGGCCCATCGTCAAC
  CACGCAGAGACTAGTCGGCTCAACATTGAGCGGATGAAGCAGATCTACCAACAGCTGTCACGATACCGGG
  GGCTCTGTCTGTTGGCATCTGTCCCTTCCTGTGTAGAAGACCTCAGTCACCCTGTGGCAGAGCCTGGTTC
  TAGCCAGGCTGGGAGCATCAGCAACACAGGCCCCAGACCACTGTCCAGTGGCCCAGCCTCCCCCAAACGC
  AAGCTGGAAGCAGCTGAGGAACCTCCAGGTGAAGAACTGAGCAAGCGGGCTAGAGTGACAGTGACTCAGA
  TGCCTGCCCAGGAGCCACCAAGCAAGGACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210144 representing NM_133405
  Red=Cloning site Green=Tags(s)
  
  MAAAAVVAATVPAQSMGADGASSVHWFRKGLRLHDNPALLAAVRGARCVRCVYILDPWFAASSSVGINRW
  RFLLQSLEDLDTSLRKLNSRLFVVRGQPADVFPRLFKEWGVTRLTFEYDSEPFGKERDAAIMKMAKEAGV
  EVVTENSHTLYDLDRIIELNGQKPPLTYKRFQALISRMELPKKPVGAVSSQHMENCRAEIQENHDDTYGV
  PSLEELGFPTEGLGPAVWQGGETEALARLDKHLERKAWVANYERPRMNANSLLASPTGLSPYLRFGCLSC
  RLFYYRLWDLYRKVKRNSTPPLSLFGQLLWREFFYTAATNNPRFDRMEGNPICIQIPWDRNPEALAKWAE
  GKTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWVSWESGVRVFDELLLDADFSVNAGSWMWLSC
  SAFFQQFFHCYCPVGFGRRTDPSGDYIRRYLPKLKGFPSRYIYEPWNAPESVQKAAKCIIGVDYPRPIVN
  HAETSRLNIERMKQIYQQLSRYRGLCLLASVPSCVEDLSHPVAEPGSSQAGSISNTGPRPLSSGPASPKR
  KLEAAEEPPGEELSKRARVTVTQMPAQEPPSKDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133405
• ORF Size: 1782 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133405.2, NP_596896.2
• RefSeq Size: 3988 bp
• RefSeq ORF: 1785 bp
• Locus ID: 170917
• Cytogenetics: 3q24
• MW: 67.1 kDa
• Gene Summary: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]