Tacc3 (NM_001004424) Rat Tagged ORF Clone

CAT#: RR210114

Tacc3 (Myc-DDK-tagged ORF) - Rat transforming, acidic coiled-coil containing protein 3 (Tacc3), (10 ug)

Product Images

Circular map for RR210114

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tacc3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210114 representing NM_001004424
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCTGCATATCTTAACTGACGAAAATGTCCCCAACGAGAAGAGTTCAGAAAGCCGTGACTTCCTGT
  TTTCAGCACCAGAACTTACCGGAAGATCCTCTGTTCTTCGCCTGTCACAGAAAGAAAATGTACCTCCCAA
  GAACCAGGCTAAAGCCGCAAAGGTGACTTTTCAGACACCTCTTCGGGATCCACAGACACACAGGATCTTA
  AGTCCTAATATGACCAGCAAACTTGAGGCTCCTTTTGCTCTGGATGACAGTGTTGGATTACAAAATGACC
  ACTGTGTCTTCTTACAGAAAGAGAACCAGCTGCCGGTAGCCCCAGTGGACGATGTACCTGTGGTTCAGAT
  GGCAGCCGAGATCCTGAAAGCAGAGGACGAGTTACAGGAGGGAATTTTGAATTCCTTGAGCCCTTCAGCT
  TTCACAAGTTTTCCAGACGGTGAGCTGGTAACCCCTCCAATCGAGCCGATGCCGGAGCCTTCCCCTCAGG
  GGCCAGAGCCTCTCTTGGACAGCGAGCCAGTGACCCCTCCAGTCAAGCCAGTGCTGGAACCTACTCCTCA
  GGGGCCAGAGCCTCTCTTGGACAGTGAGCCGGTGACCCCTCCAGTCAAGCCAGTACTGGAACCTACCCCT
  CAGGGATCAGAGCCTCTCTTGGACAGTGAGCCAGTGACCCTTCCTGTTGAACCAGTGCCAGAGTCTTCCC
  ATCAGGGACCAGAACCTCTCTTGGACAGTGAGCCAGTGACCCTTCCTGTTGAACCAGTGCCAGAGTCTTC
  CCATCAGGGACCAGAACCTCTCTTGGACCCAAAGGAAGAGAGCTTTAGAGACCCAGCAGAAGTTCTAGGT
  GCTGGTGCTGAAGTGGATTATCTGGAGCAATTTGGAACTTCCTCGTTCAAGGAGTCAGCCTGGAGGAAGC
  AGTCTTTGTACTTGAAATTTGACCCCCTTCTGAAAGAGAGCCCTTTGCGGTCAATGCCAGTGCCAGTGGC
  CCCAGTGACAAATAGTACACAGGATACAGAGGAGTCTGGCTCCGGAAATCCAACAGAAGCCAAGCTCGTC
  GACTTAGATTTCTTGGGAGGTCTGGATGTTCCTGTGTCGGCCCCACCCCTGTGTGTCCTTGAGCCTCGAG
  GTCTCCTTCCTGCCGAGCCTATTGTGGACGTGCTGAAATACAGTCAGAAAGACCTGGATGCAGTGGTGAT
  GCAGCAGGAGAACTTGGAGCTGAGAAACAAATATGAGGACCTCAACACGAAGTATCTGGAGATGGGAAGA
  TCGTTGATGGATTTGAGAAAGATCGTGTATAAATCAATGGAGGAGGCTGAGAAACAGAAGGAACTAGCTG
  AAGACAAAATTCAAAAGATTTTAAGAGAGAGAGACCAACTTACTGCGGATCTGAACTCTATGGAGAAGTC
  CTTCTCTGACCTCTTCAAGCGGTTTGAGAAGCAGAAGGAAGTGATTGAAGGCTACCAGAAGAATGAAGAT
  TCACTGAAGAAGTGTGTTGAGGATTACATAGTGAAGATTGGAAAAGAAGGCCAGAGGTACCAGGCGCTGA
  AGGCCCACGCAGAAGAAAAACTCAAGATGGCAAATGAAGAGATCGCCCAAGTGCGAAGCAAGGCCCAAGC
  AGAGGCACTGGCTCTCCAGGCAAGCCTGAGGAAGGCACAGTTGCAGATCCAGTCACTGGAGAAGACTGTG
  GAGCAGAAGACTAAAGAGAACGATGAACTGAACAGGATCTGCGATGAACTCATTGCTAAGATGGAGAAGA
  AGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210114 representing NM_001004424
  Red=Cloning site Green=Tags(s)
  
  MSLHILTDENVPNEKSSESRDFLFSAPELTGRSSVLRLSQKENVPPKNQAKAAKVTFQTPLRDPQTHRIL
  SPNMTSKLEAPFALDDSVGLQNDHCVFLQKENQLPVAPVDDVPVVQMAAEILKAEDELQEGILNSLSPSA
  FTSFPDGELVTPPIEPMPEPSPQGPEPLLDSEPVTPPVKPVLEPTPQGPEPLLDSEPVTPPVKPVLEPTP
  QGSEPLLDSEPVTLPVEPVPESSHQGPEPLLDSEPVTLPVEPVPESSHQGPEPLLDPKEESFRDPAEVLG
  AGAEVDYLEQFGTSSFKESAWRKQSLYLKFDPLLKESPLRSMPVPVAPVTNSTQDTEESGSGNPTEAKLV
  DLDFLGGLDVPVSAPPLCVLEPRGLLPAEPIVDVLKYSQKDLDAVVMQQENLELRNKYEDLNTKYLEMGR
  SLMDLRKIVYKSMEEAEKQKELAEDKIQKILRERDQLTADLNSMEKSFSDLFKRFEKQKEVIEGYQKNED
  SLKKCVEDYIVKIGKEGQRYQALKAHAEEKLKMANEEIAQVRSKAQAEALALQASLRKAQLQIQSLEKTV
  EQKTKENDELNRICDELIAKMEKKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004424
• ORF Size: 1755 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004424.1, NP_001004424.1
• RefSeq Size: 2042 bp
• RefSeq ORF: 1758 bp
• Locus ID: 360962
• Cytogenetics: 14q21
• MW: 65 kDa
• Gene Summary: This gene encodes a member of the transforming, acidic coiled-coil (TACC) family of proteins. Members of this family are centrosomal proteins that interact with microtubules and tubulin. TACC proteins are thought to be involved in centrosome/mitotic spindle dynamics and gene regulation. [provided by RefSeq, Jul 2008]