Prkaa1 (NM_019142) Rat Tagged ORF Clone

CAT#: RR210018

Prkaa1 (Myc-DDK-tagged ORF) - Rat protein kinase, AMP-activated, alpha 1 catalytic subunit (Prkaa1), (10 ug)

Product Images

Circular map for RR210018

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prkaa1
• Synonyms: AMPKalpha1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210018 representing NM_019142
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCAGACTCAGTTCCTGGAGAAAGATGGCGACGGCCGAGAAGCAGAAGCACGACGGGCGGGTGAAGA
  TCGGCCACTACATCCTGGGGGACACGCTGGGCGTCGGCACCTTCGGGAAAGTGAAGGTGGGCAAGCACGA
  GTTGACTGGACATAAAGTTGCTGTGAAGATACTCAACCGGCAGAAGATTCGAAGCCTGGACGTGGTCGGG
  AAAATCCGCAGAGAGATCCAGAACCTGAAGCTTTTCAGGCACCCTCATATAATCAAACTGTACCAGGTCA
  TCAGTACACCGTCTGATATTTTCATGGTCATGGAATATGTCTCAGGAGGAGAGCTATTTGATTATATCTG
  TAAAAATGGAAGGTTGGACGAAAAGGAGAGTCGACGTCTGTTCCAGCAGATCCTTTCTGGTGTGGACTAT
  TGTCACAGGCATATGGTGGTCCACAGAGATTTGAAACCTGAAAACGTCCTGCTTGATGCACACATGAATG
  CAAAGATAGCCGACTTCGGTCTTTCAAACATGATGTCAGATGGTGAATTTTTAAGAACGAGCTGTGGCTC
  GCCCAATTATGCTGCACCAGAAGTAATTTCAGGAAGATTGTACGCAGGCCCTGAAGTAGACATCTGGAGC
  AGCGGGGTCATTCTCTATGCTTTGCTGTGTGGAACTCTCCCTTTTGATGATGACCACGTGCCAACTCTTT
  TTAAGAAGATATGTGACGGGATATTTTATACCCCTCAGTATTTGAATCCCTCTGTAATAAGCCTTTTGAA
  GCATATGCTGCAGGTAGATCCTATGAAGAGGGCCACAATAAAAGATATCAGGGAACATGAATGGTTTAAG
  CAGGACCTTCCAAAATATCTCTTTCCTGAAGACCCGTCTTATAGTTCAACCATGATTGATGATGAAGCCT
  TAAAAGAAGTGTGTGAGAAGTTCGAGTGCTCAGAGGAGGAGGTCCTCAGCTGCCTGTACAACAGAAACCA
  CCAGGACCCACTGGCAGTTGCCTACCACCTCATAATAGACAACAGGAGAATAATGAACGAAGCCAAAGAT
  TTCTACTTGGCAACAAGCCCACCCGATTCTTTCCTCGATGATCACCATTTAACTCGGCCTCACCCTGAGA
  GAGTACCATTCTTGGTTGCCGAAACACCAAGGGCCCGACACACCCTAGATGAATTAAACCCACAGAAATC
  CAAACACCAAGGCGTACGGAAGGCAAAGTGGCATTTGGGGATTCGAAGTCAAAGCCGACCCAATGACATC
  ATGGCAGAAGTGTGTAGAGCAATCAAGCAGTTGGACTATGAATGGAAGGTTGTAAACCCCTATTATTTGC
  GTGTGCGAAGGAAGAACCCTGTGACAAGCACATTTTCCAAAATGAGTCTACAGCTATACCAAGTGGATAG
  TAGGACTTACTTATTGGATTTCCGAAGTATTGATGATGAGATTACAGAAGCCAAATCAGGGACTGCTACT
  CCACAGAGATCGGGATCCATCAGCAACTATCGATCTTGCCAAAGGAGCGACTCCGACGCCGAGGCTCAAG
  GAAAGCCCTCAGAAGTCTCTCTTACCTCATCCGTGACCTCCCTCGACTCCTCTCCTGTTGACGTAGCTCC
  AAGACCAGGAAGTCACACGATAGAATTTTTTGAAATGTGTGCAAATCTAATTAAAATTCTTGCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210018 representing NM_019142
  Red=Cloning site Green=Tags(s)
  
  MRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVG
  KIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDY
  CHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWS
  SGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFK
  QDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKD
  FYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDI
  MAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTFSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTAT
  PQRSGSISNYRSCQRSDSDAEAQGKPSEVSLTSSVTSLDSSPVDVAPRPGSHTIEFFEMCANLIKILAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019142
• ORF Size: 1677 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019142.2, NP_062015.2
• RefSeq Size: 4674 bp
• RefSeq ORF: 1680 bp
• Locus ID: 65248
• UniProt ID: P54645
• Cytogenetics: 2q16
• MW: 64 kDa
• Gene Summary: catalytic subunit of AMP-activated protein kinase; phosphorylates hepatic acetyl-CoA carboxylase and adipose hormone-sensitive lipase [RGD, Feb 2006]