Ofd1 (NM_001106961) Rat Tagged ORF Clone

CAT#: RR209994

Ofd1 (Myc-DDK-tagged ORF) - Rat oral-facial-digital syndrome 1 gene homolog (human) (Ofd1), (10 ug)

Product Images

Circular map for RR209994

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ofd1
• Synonyms: RGD1562231
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209994 representing NM_001106961
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGTCCACCATGCCCCACAAGTGTGATGATGTGTTGAGTCAAGATGAACTGCGCAAAAAGCTAT
  ACCAGACGTTTAAGGATCGGGGTATACTGGACACACTTAAGACACAACTCCGAAACCAGTTAGTTCATGA
  ATTGATGCATCCTGTACTGAGTGGAGAAGTGAAGCCCCCTTCCATTTCAGTGGAAGGGAGTTCCCTACTA
  ATAGGCGCTTCTAACTCGCTGGTGGCAGATCACTTACAAAGATGTGGCTATGAGTATTCACTTTCTGTTT
  TCTTTCCAGAAAGTGGTTTGGCAAAAGAAAAGATATTTACTATGCAGGATTTACTACAACTCATTCGGAT
  TAACCCTTCATCCAGTCTCTATAAATCATTGATTTCAGGATTTGATAAAGACAACCAAAAAGGTTTTCTT
  ATGAGTTTCTTAAAGGAGTTGGCAGAATATCATCAAACTAAAGAGAGCTGTGATGTGGAAACTCAGACAA
  GCACCACATTTCCTAGCCAAGTGTCCCTAGCCGAGAAGTTTCAGCTTATCGATGATCAATTTGCAGAAGC
  TTTTCCACATCGTCCCAAGCTAGAATCTTTAGAGACCAAGTTAAATGAGTATAAGAAAGAAATACAGCAG
  CAGCTTCAGGTAGAAATGTGTCAGAAGCTGAAGTATTTTAGAGAAGCTGAAATAACTAAAGTTAGGATGG
  AAGAGAAAAGAAAATATGAAAAGGAATTGGCTGAGTTCCGGAATGAATTTGAAAGAACTTGTCAAGCAAA
  AAATGAAGCCCTCATTTCTCAGGAAAAGAACACTCTGGAAAGAATCAAAAAGCACCAAGAGATGGAAACA
  AAAGAAATTTATGCTCAGAGACAGCTTCTACTAAATGATATAGATTTGCTTAGAGGTAGAGAAGCAGAGC
  TGAAGGAACGAATCGAAGCTTTTGAATTGACCCAGAAGCTCCAAGAAGAAAAAAATAAAAATGAGGCTGA
  GGCACTTCGGAGACGGGAACAGAATCTAAAGAATATTGAAGAGGCCTATGAACAGAAACTCAAAACTGAA
  CTTCTAAAGTACCAACTGGAACTAAAGGATGATTATATCACCAGAACAAATAAACTCCTGGAAGAGGAAA
  GGAAGAATAAAGAAAAAACTTTTCATTTGCAAGAGGAGCTCACAGTTATTAATTCAAAAAAGGAAGAACT
  CAGTAAATCTGTACAGCGTATGAAAGAAGTTGAGCTGGAGCTGGAGTCTGTCAAAGCTCAATTTTTTGCA
  ATGTCAAAACAAAACCACTCGCTGAATGAAAAACTGAAAGAGATGAGTGATTACTCACAACTGAAAGAAG
  AGAAAGTGGAACTTCAGGCACAAAATAAATTACTTAAACAGCAACTGGAAGAGAATAGGAATGAAAACTT
  ACGTCTCCTAGACCGTATAACTCAGCCACCCCCTGAGCTTTTAGTTTTTCAGAAAGAATTACAGAAAACA
  GAAAAGGCTATGGCACTGGAGCATAAAGGCTTTGAAACTCATAGGCAAGCTCTGCAGAAACAGCTGCAGA
  GTGAAATTGAAAATTCTACACAGCTAAGGACCCAGATATCAGAATATGATGCTTCTGTTAAAAGGTTAAC
  TGTTCAGGTTGCTGATTTAAAGTCACAACTAAAGCAAACTCAGACAGCCCTAGAGAACGAAGTGTTCCGA
  AATCCAAAGCGTTCTCTGACCCTTCATTCCCTGACTGGCCTATTGAGTGGCAAGATGGCACCCCACAATG
  AAGATACAAGTGGGGATTTCTTGAATGTACCCCTTGAACAGAACAAGGTTATGGCAGGTGCAGTTGTGTC
  AAGGGTCACACCTTATGTAAATACAGCAACAGAGGCTAGTTCCCCAGACTCTGATCTCGAGTTTGTAGCC
  AGTAGTACAAAAGCCAAAGTAAAAGAACTAGAGCAAGAGGCTGAGCGCTTGGAAAAGGCCTTCAGAACTT
  ACTATCAAAGAGTTACTCAGAATCCTTCTATGAGCCCTCAGCCGGCAAAGAGTCCTTCATCAGTATACTC
  TGTAGGAGCTCTCAGAAGCATAGCTTCCAGTTCCAGGGACAGACATGTTCCTGCAGAGGACAGAGTTGTC
  TCTGAACAGCCTCTTGCAGACATACTAAAGGAAGAAAAGAGTGATGTGTCCAAAGCGTTCGTGGGCAGCA
  TGGTTTCACGGCCACGCAGGACCTCTTCCTCCACACGCCTCTCCTCTACACCGCATCCAAAATCGAGAAG
  AAGTCTTGATAATGAAATGTATCTGGAAGGTCTCAGGAGATTACACATTACTTCCTCCAGCCCTTGTCTT
  GACAGACCATCTGAGTCACCTGCTGCTTCCCCTACTCCTTGTCCTGAGAGAACACCCCAGCCATCATCGG
  TTCCATCTAGGCACAGCTTCTCTGGTCCATCTTTCTCCAGCCCTCCAGAGCAAAATGCCTCTTTATATCA
  GAGACAAACTGAAATGCAAGATAAAAGTGAGTTTTCAAATGTGGACAAGCAATCTTTGAAAGATAAGGAG
  AAATTTGAACCATCTTTCAGATGGAACAAGCCAGAACAGTTTGAAGCAGAAGGACTCCACCCTGCTGGTG
  ACATGCCTGGCATAGATGTTGCTGCAGCTACTCTGTCTTCTAGACCCATTTCATACAACCACCCAAGTGG
  TGCTGTTCAGAGCCAAACTGGAGAACAAAAAGAACAGCAGATATGGGAACTCCACATGAAAGAACGCAGA
  CAGAGAGAAGAACAAAGGCAGAATGAACGGCAAGAAGCTCTAGAGAGGGAAAGAAGAGAGCTAGAAAAAC
  TAGAGCAGGAGCGGAGGATGATTGAAGAGTCACTGAAGAGTGAGATAGAAGAAGAATTAGAAAAGAGTGT
  TCAAGAACAGAAAGACAAATCAGCTCATGGAGAGAATCCGTTAGAAAAGTACATGAAAATCATCCAGCAG
  AGACAGGAACAATCAAGCGCAGATAAGAGCTCCAAAAAATCGGGCAAAGAATGCTCACTAGTGGACATGA
  TGATACCTAGTGACAAGGATGACAGTTCTCCAGGCTTTTCCCATGAAGAGCCAGATGACATCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209994 representing NM_001106961
  Red=Cloning site Green=Tags(s)
  
  MAQSTMPHKCDDVLSQDELRKKLYQTFKDRGILDTLKTQLRNQLVHELMHPVLSGEVKPPSISVEGSSLL
  IGASNSLVADHLQRCGYEYSLSVFFPESGLAKEKIFTMQDLLQLIRINPSSSLYKSLISGFDKDNQKGFL
  MSFLKELAEYHQTKESCDVETQTSTTFPSQVSLAEKFQLIDDQFAEAFPHRPKLESLETKLNEYKKEIQQ
  QLQVEMCQKLKYFREAEITKVRMEEKRKYEKELAEFRNEFERTCQAKNEALISQEKNTLERIKKHQEMET
  KEIYAQRQLLLNDIDLLRGREAELKERIEAFELTQKLQEEKNKNEAEALRRREQNLKNIEEAYEQKLKTE
  LLKYQLELKDDYITRTNKLLEEERKNKEKTFHLQEELTVINSKKEELSKSVQRMKEVELELESVKAQFFA
  MSKQNHSLNEKLKEMSDYSQLKEEKVELQAQNKLLKQQLEENRNENLRLLDRITQPPPELLVFQKELQKT
  EKAMALEHKGFETHRQALQKQLQSEIENSTQLRTQISEYDASVKRLTVQVADLKSQLKQTQTALENEVFR
  NPKRSLTLHSLTGLLSGKMAPHNEDTSGDFLNVPLEQNKVMAGAVVSRVTPYVNTATEASSPDSDLEFVA
  SSTKAKVKELEQEAERLEKAFRTYYQRVTQNPSMSPQPAKSPSSVYSVGALRSIASSSRDRHVPAEDRVV
  SEQPLADILKEEKSDVSKAFVGSMVSRPRRTSSSTRLSSTPHPKSRRSLDNEMYLEGLRRLHITSSSPCL
  DRPSESPAASPTPCPERTPQPSSVPSRHSFSGPSFSSPPEQNASLYQRQTEMQDKSEFSNVDKQSLKDKE
  KFEPSFRWNKPEQFEAEGLHPAGDMPGIDVAAATLSSRPISYNHPSGAVQSQTGEQKEQQIWELHMKERR
  QREEQRQNERQEALERERRELEKLEQERRMIEESLKSEIEEELEKSVQEQKDKSAHGENPLEKYMKIIQQ
  RQEQSSADKSSKKSGKECSLVDMMIPSDKDDSSPGFSHEEPDDIW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106961
• ORF Size: 3075 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106961.1, NP_001100431.1
• RefSeq Size: 3561 bp
• RefSeq ORF: 3078 bp
• Locus ID: 302661
• Cytogenetics: Xq13
• MW: 118.3 kDa