Nos1 (NM_052799) Rat Tagged ORF Clone

CAT#: RR209715

Nos1 (Myc-DDK-tagged ORF) - Rat nitric oxide synthase 1, neuronal (Nos1), (10 ug)

Product Images

Circular map for RR209715

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nos1
• Synonyms: bNOS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209715 representing NM_052799
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGAACACGTTTGGGGTTCAGCAGATCCAACCCAATGTAATTTCTGTTCGTCTCTTCAAACGCA
  AAGTGGGAGGTCTGGGCTTCCTGGTGAAGGAACGGGTCAGCAAGCCTCCCGTGATCATCTCAGACCTGAT
  TCGAGGAGGTGCTGCGGAGCAGAGCGGCCTTATCCAAGCTGGAGACATCATTCTCGCAGTCAACGATCGG
  CCCTTGGTAGACCTCAGCTATGACAGTGCCCTGGAGGTTCTCAGGGGCATTGCCTCTGAGACCCACGTGG
  TCCTCATTCTGAGGGGCCCTGAGGGCTTCACTACACATCTGGAGACCACCTTCACAGGGGATGGAACCCC
  CAAGACCATCCGGGTGACCCAGCCCCTCGGTCCTCCCACCAAAGCCGTCGATCTGTCTCACCAGCCTTCA
  GCCAGCAAAGACCAGTCATTAGCAGTAGACAGAGTCACAGGTCTGGGTAATGGCCCTCAGCATGCCCAAG
  GCCATGGGCAGGGAGCTGGCTCAGTCTCCCAAGCTAATGGTGTGGCCATTGACCCCACGATGAAAAGCAC
  CAAGGCCAACCTCCAGGACATCGGGGAACATGATGAACTGCTCAAAGAGATAGAACCTGTGCTGAGCATC
  CTCAACAGTGGGAGCAAAGCCACCAACAGAGGGGGACCAGCCAAAGCAGAGATGAAAGACACAGGAATCC
  AGGTGGACAGAGACCTCGATGGCAAATCGCACAAAGCTCCGCCCCTGGGCGGGGACAATGACCGCGTCTT
  CAATGACCTGTGGGGGAAGGACAACGTTCCTGTGGTCCTTAACAACCCGTATTCAGAGAAGGAACAGTCC
  CCTACCTCGGGGAAACAGTCTCCCACCAAGAACGGCAGCCCTTCCAGGTGCCCCCGTTTCCTCAAGGTCA
  AGAACTGGGAGACGGACGTGGTCCTCACCGACACCCTGCACCTGAAGAGCACACTGGAAACGGGGTGCAC
  AGAGCACATTTGCATGGGCTCGATCATGCTGCCTTCCCAGCACACGCGGAAGCCAGAAGATGTCCGCACA
  AAGGACCAGCTCTTCCCTCTAGCCAAAGAATTTCTCGACCAATACTACTCATCCATTAAGAGATTTGGCT
  CCAAGGCCCACATGGACAGGCTGGAGGAGGTGAACAAGGAGATTGAAAGCACCAGCACCTACCAGCTCAA
  GGACACCGAGCTCATCTATGGCGCCAAGCATGCCTGGCGGAACGCCTCTCGATGTGTGGGCAGGATCCAG
  TGGTCCAAGCTGCAGGTGTTCGATGCCCGAGACTGCACCACAGCCCACGGCATGTTCAACTACATCTGTA
  ACCATGTCAAGTATGCCACCAACAAAGGGAATCTCAGGTCGGCCATCACGATATTCCCTCAGAGGACTGA
  CGGCAAACATGACTTCCGAGTGTGGAACTCGCAGCTCATCCGCTACGCGGGCTACAAGCAGCCAGATGGC
  TCTACCTTGGGGGATCCAGCCAATGTGCAGTTCACGGAGATCTGTATACAGCAGGGCTGGAAAGCCCCAA
  GAGGCCGCTTCGACGTGCTGCCTCTCCTGCTTCAGGCCAATGGCAATGACCCTGAGCTCTTCCAGATCCC
  CCCAGAGCTGGTGCTGGAAGTGCCCATCAGGCACCCCAAGTTCGACTGGTTTAAGGACCTGGGGCTCAAA
  TGGTATGGCCTCCCCGCTGTGTCCAACATGCTGCTGGAGATCGGGGGCCTGGAGTTCAGCGCCTGTCCCT
  TCAGCGGCTGGTACATGGGCACAGAGATCGGCGTCCGTGACTACTGTGACAACTCTCGATACAACATCCT
  GGAGGAAGTAGCCAAGAAGATGGATTTGGACATGAGGAAGACCTCGTCCCTCTGGAAGGACCAAGCACTG
  GTGGAGATCAACATTGCTGTTCTATATAGCTTCCAGAGTGACAAGGTGACCATCGTTGACCACCACTCTG
  CCACGGAGTCCTTCATCAAACACATGGAGAATGAATACCGCTGCAGAGGGGGCTGCCCCGCCGACTGGGT
  GTGGATTGTGCCTCCCATGTCGGGCAGCATCACCCCTGTCTTCCACCAGGAGATGCTCAACTATAGACTC
  ACCCCGTCCTTTGAATACCAGCCTGATCCATGGAACACCCACGTGTGGAAGGGCACCAACGGGACCCCCA
  CGAAGCGGCGAGCTATCGGCTTTAAGAAATTGGCAGAGGCCGTCAAGTTCTCAGCCAAGCTAATGGGGCA
  GGCCATGGCCAAGAGGGTCAAGGCGACCATTCTCTACGCCACAGAGACAGGCAAATCACAAGCCTATGCC
  AAGACCCTGTGTGAGATCTTCAAGCACGCCTTCGATGCCAAGGCAATGTCCATGGAGGAGTATGACATCG
  TGCACCTGGAGCACGAAGCCCTGGTCTTGGTGGTCACCAGCACCTTTGGCAATGGAGACCCCCCTGAGAA
  CGGGGAGAAATTCGGCTGTGCTTTAATGGAGATGAGGCACCCCAACTCTGTGCAGGAGGAGAGAAAGTAC
  CCGGAACCCTTGCGTTTCTTTCCCCGTAAAGGGCCTTCCCTCTCCCATGTTGACTCTGAAGCCCACAGTC
  TGGTTGCTGCCCGTGACAGCCAACACAGGAGCTACAAGGTCCGATTCAACAGCGTCTCCTCCTATTCTGA
  CTCCCGAAAGTCATCGGGCGACGGACCCGACCTCAGAGACAACTTTGAAAGTACTGGACCCCTGGCCAAT
  GTGAGGTTCTCAGTGTTCGGCCTCGGCTCTCGGGCGTACCCCCACTTCTGTGCCTTTGGGCATGCGGTGG
  ACACCCTCCTGGAGGAACTGGGAGGGGAGAGGATTCTGAAGATGAGGGAGGGGGATGAGCTTTGCGGACA
  GGAAGAAGCTTTCAGGACCTGGGCCAAGAAAGTCTTCAAGGCAGCCTGTGATGTGTTCTGCGTGGGGGAT
  GACGTCAACATCGAGAAGGCGAACAACTCCCTCATTAGCAATGACCGAAGCTGGAAGAGGAACAAGTTCC
  GCCTCACGTATGTGGCGGAAGCTCCAGATCTGACCCAAGGTCTTTCCAATGTTCACAAAAAACGAGTCTC
  GGCTGCTCGACTCCTCAGCCGCCAAAACCTGCAAAGCCCTAAGTCCAGCCGATCGACCATCTTCGTGCGT
  CTCCACACCAACGGGAATCAGGAGCTGCAGTACCAGCCAGGGGACCACCTGGGTGTCTTCCCCGGCAACC
  ACGAGGACCTCGTGAATGCACTCATTGAACGGCTGGAGGATGCACCGCCTGCCAACCACGTGGTGAAGGT
  GGAGATGCTGGAGGAGAGGAACACTGCTCTGGGTGTCATCAGTAATTGGAAGGATGAATCTCGCCTCCCA
  CCCTGCACCATCTTCCAGGCCTTCAAGTACTACCTGGACATCACCACGCCGCCCACGCCCCTGCAGCTGC
  AGCAGTTCGCCTCTCTGGCCACTAATGAGAAAGAGAAGCAGCGGTTGCTGGTCCTCAGCAAGGGGCTCCA
  GGAATATGAGGAGTGGAAGTGGGGCAAGAACCCCACAATGGTGGAGGTGCTGGAGGAGTTCCCGTCCATC
  CAGATGCCGGCTACACTTCTCCTCACTCAGCTGTCGCTGCTGCAGCCTCGCTACTACTCCATCAGCTCCT
  CTCCAGACATGTACCCCGACGAGGTGCACCTCACTGTGGCCATCGTCTCCTACCACACCCGAGACGGAGA
  AGGACCAGTCCACCACGGGGTGTGCTCCTCCTGGCTCAACAGAATACAGGCTGACGATGTAGTCCCCTGC
  TTCGTGAGAGGTGCCCCTAGCTTCCACCTGCCTCGAAACCCCCAGGTGCCTTGCATCCTGGTTGGCCCAG
  GCACTGGCATCGCACCCTTCCGAAGCTTCTGGCAACAGCGACAATTTGACATCCAACACAAAGGAATGAA
  TCCGTGCCCCATGGTTCTGGTCTTCGGGTGTCGACAATCCAAGATAGATCATATCTACAGAGAGGAGACC
  CTGCAGGCCAAGAACAAGGGCGTCTTCAGAGAGCTGTACACTGTCTATTCCCGGGAACCGGACAGGCCAA
  AGAAATATGTACAGGACGTGCTGCAGGAACAGCTGGCTGAGTCTGTGTACCGCGCCCTGAAGGAGCAAGG
  AGGCCACATTTATGTCTGTGGGGACGTTACCATGGCCGCCGATGTCCTCAAAGCCATCCAGCGCATAATG
  ACCCAGCAGGGGAAACTCTCAGAGGAGGACGCTGGTGTATTCATCAGCAGGCTGAGGGATGACAACCGGT
  ACCACGAGGACATCTTTGGAGTCACCCTCAGAACGTATGAAGTGACCAACCGCCTTAGATCTGAGTCCAT
  CGCCTTCATCGAAGAGAGCAAAAAAGACGCAGATGAGGTTTTCAGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209715 representing NM_052799
  Red=Cloning site Green=Tags(s)
  
  MEENTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDR
  PLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPS
  ASKDQSLAVDRVTGLGNGPQHAQGHGQGAGSVSQANGVAIDPTMKSTKANLQDIGEHDELLKEIEPVLSI
  LNSGSKATNRGGPAKAEMKDTGIQVDRDLDGKSHKAPPLGGDNDRVFNDLWGKDNVPVVLNNPYSEKEQS
  PTSGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEHICMGSIMLPSQHTRKPEDVRT
  KDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQ
  WSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDG
  STLGDPANVQFTEICIQQGWKAPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLK
  WYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQAL
  VEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRL
  TPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYA
  KTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKY
  PEPLRFFPRKGPSLSHVDSEAHSLVAARDSQHRSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLAN
  VRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGD
  DVNIEKANNSLISNDRSWKRNKFRLTYVAEAPDLTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVR
  LHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLP
  PCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSI
  QMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPC
  FVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREET
  LQAKNKGVFRELYTVYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIM
  TQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDADEVFSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052799
• ORF Size: 4389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052799.1, NP_434686.1
• RefSeq Size: 4592 bp
• RefSeq ORF: 4392 bp
• Locus ID: 24598
• UniProt ID: P29476
• Cytogenetics: 12q16
• MW: 164.4 kDa
• Gene Summary: enzyme that catalyzes the production of nitric oxide [RGD, Feb 2006]