Pdgfc (NM_031317) Rat Tagged ORF Clone

CAT#: RR209673

Pdgfc (Myc-DDK-tagged ORF) - Rat platelet derived growth factor C (Pdgfc), (10 ug)

Product Images

Circular map for RR209673

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pdgfc
• Synonyms: SCDGF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209673 representing NM_031317
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCTCCTCGGCCTCCTCCTGCTGACATCTGCCCTGGCCGGCCAGAGAACCGGGACTCGGGCTGAGT
  CCAACCTGAGCAGCAAGTTGCAGCTCTCCAGCGACAAGGAGCAGAACGGAGTGCAAGATCCCCGGCATGA
  GAGAGTTGTCACTATATCTGGTAATGGGAGCATCCACAGCCCGAAGTTTCCTCATACCTACCCAAGAAAT
  ACGGTGCTGGTCTGGAGATTAGTGGCAGTAGATGAAAATGTGCGGATCCAGCTGACATTTGATGAGAGAT
  TTGGGCTGGAAGACCCAGAGGACGATCTATGCAAGTATGATTTTGTAGAAGTTGAGGAGCCCAGTGATGG
  AAGTGTATTAGGACGCTGGTGTGGTTCTGGGACTGTGCCAGGAAAGCAGACTTCTAAAGGAAATCATATC
  AGGATAAGATTTGTATCTGATGAGTATTTCCCATCTGAACCCGGATTCTGCATCCACTACAGTATTATCA
  TGCCACAAGTCACAGAAACTACGAGTCCTTCAGTGTTGCCCCCTTCAGCTTTGTCATTGGACCTGCTCAA
  TAATGCTGTGACTGCCTTCAGTACTGTGGAAGAGCTGATTCGGTTCCTAGAGCCAGATCGATGGCAGATA
  GACTTGGACAGCCTCTACAAGCCAACATGGCCGCTTTTGGGCAAAGCTTTCCTGTATGGGAAAAAAAGCA
  AAGCGGTGAATCTGAACCTTCTCAAAGAAGAGGTAAAACTTTACAGCTGCACACCCCGGAACTTCTCTGT
  GTCCATACGGGAAGAGCTAAAGAGGACAGACACCATATTCTGGCCAGGTTGTCTCCTGGTCAAGCGCTGT
  GGAGGAAATTGTGCCTGTTGCCTCCATAATTGCAATGAATGTCAGTGTGTCCCACGTAAAGTTACAAAAA
  AGTACCATGAGGTCCTTCAGTTGAGACCAAAAATTGGAGTCAAGGGATTGCATAAGTCGCTCACCGATGT
  GGCCCTGGAACACCATGAGGAATGTGACTGTGTGTGTAGAGGAAACACAGAAGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209673 representing NM_031317
  Red=Cloning site Green=Tags(s)
  
  MLLLGLLLLTSALAGQRTGTRAESNLSSKLQLSSDKEQNGVQDPRHERVVTISGNGSIHSPKFPHTYPRN
  TVLVWRLVAVDENVRIQLTFDERFGLEDPEDDLCKYDFVEVEEPSDGSVLGRWCGSGTVPGKQTSKGNHI
  RIRFVSDEYFPSEPGFCIHYSIIMPQVTETTSPSVLPPSALSLDLLNNAVTAFSTVEELIRFLEPDRWQI
  DLDSLYKPTWPLLGKAFLYGKKSKAVNLNLLKEEVKLYSCTPRNFSVSIREELKRTDTIFWPGCLLVKRC
  GGNCACCLHNCNECQCVPRKVTKKYHEVLQLRPKIGVKGLHKSLTDVALEHHEECDCVCRGNTEG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031317
• ORF Size: 1035 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031317.1, NP_112607.1
• RefSeq Size: 1116 bp
• RefSeq ORF: 1038 bp
• Locus ID: 79429
• UniProt ID: Q9EQX6
• Cytogenetics: 2q33-q34
• MW: 38.7 kDa
• Gene Summary: expressed in the embryonic spinal cord floor plate and ventricular zone, adult expression is localized to motoneurons in several regions of the brain [RGD, Feb 2006]