Chpf (NM_001005906) Rat Tagged ORF Clone

CAT#: RR209665

Chpf (Myc-DDK-tagged ORF) - Rat chondroitin polymerizing factor (Chpf), (10 ug)

Product Images

Circular map for RR209665

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chpf
• Synonyms: D1bwg1363e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209665 representing NM_001005906
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGCGTCGCTGCTGCTGTCCGTGCTGCGGCCTGCGGGGCCGGTGGCCGTGGGCATCTCTCTGGGCT
  TCACCCTGAGCTTACTCAGCGTCACCTGGGTGGAGGAGCCTTGCGGACCCGGACCACCCCAACCCGGCGA
  CTCTGAACTACCGCCGCGCGGCAACACCAACGCGGCGCGCCGGCCCAACTCGGTGCAGCCCGGAGCGGAG
  CGCGAGAGGCCCGGGGCCGACGAAAGCTGGGAGCCTCGTGTCTTGCCCTACCATCCCGCCCAGCCAGGCC
  AGGCTACCAAGAAGGCCGTCAGAACTCGGTATATCAGCACGGAACTGGGCATCAGGCAGAAGCTGCTAGT
  GGCAGTGCTGACCTCACAGGCCACGTTGCCTACACTGGGTGTGGCTGTAAACCGAACTCTGGGACACCGA
  TTGGAGCATGTAGTGTTCCTGACCGGTGCGAGGGGCCGCCGGACACCATCAGGCATGGCGGTGGTGGCAC
  TGGGCGAAGAGCGGCCCATCGGACACCTGCACCTGGCGCTGCGCCACCTGCTGGAGCAACACGGCGATGA
  CTTTGACTGGTTTTTCCTAGTGCCTGATGCCACCTACACTGAGGCGCATGGACTGGACCGCCTAACTGGC
  CACCTCAGCCTTGCTTCAGCAACCCACCTCTATCTTGGCCGACCCCAGGACTTCATCGGTGGAGAGACTA
  CCCCAGGCCGTTACTGCCACGGGGGCTTTGGAGTGTTGCTGTCTCGCACACTACTACAGCAACTGCGCCC
  CCACCTGGAAAGCTGCCGTAATGACATCGTCAGTGCTCGCCCAGATGAGTGGTTGGGCCGGTGCATCCTT
  GATGCCACAGGCATTGGCTGTACTGGTCACCATGAGGGAATGCACTACAACTATCTGGAACTGAGCCCTG
  GGGAACCTGTGCAGGAGGGGGACCCTCGTTTCCGCAGCGCCTTGACAGCCCACCCTGTGCATGACCCTGT
  GCACATGTACCAGCTGCACAAAGCTTTTGCCCGGGCTGAGCTGGACCGCACATACCAGGAGATTCAAGAA
  CTGCAGTGGGAGATCCAGAATACCAGCAGACTGGCTGCTGATGGGGAGAGGGCTTCTACCTGGCCAGTGG
  GCATTCCAGCGCCGTCTCGCCCTGCCTCGCGCTTTGAGGTTCTGCGCTGGGACTACTTCACGGAACAGCA
  CACTTTCTCCTGTGCTGACGGCTCACCCCGCTGCCTGCTGCGTGGGGCCGACCAGGCCGATGTGGCTGAT
  GTCCTAGGAACAGCCTTAGAGGAGCTCAACCGCCGTTACCAGCCAGCACTGCGGCTCCAGAAGCAACAGC
  TGGTGAATGGCTACCGGCGTTTTGATCCAGCCCGAGGTATGGAGTACACACTAGACTTGCAGCTGGAAGC
  GCTGACGCCCCAGGGTGGCCGCTGGCCCCTCACCCACAGGGTACAGCTCCTTCGGCCCTTGAGCCGAGTG
  GAGATCTTGCCTGTGCCCTATGTCACCGAGGCTTCTCGGGTCACTGTGCTGCTGCCTCTGGCTGCAGCAG
  AGTGTGACCTGGCATCTGGCTTCCTAGAAGCCTTTGCCACTGCAGCACTGGAACCTGGCGATGCCGCAGC
  CTTGACCCTGCTGCTGCTGTATGAGCCACGCCAGGCCCAGCGGGCAGCCCACGCAGATGTCTTCGCACCC
  GTCAAAGCCCACGTGGCCGAGCTAGAGCGGCGTTTCCCTGGTGCCCGAGTGCCCTGGCTCAGTGTACAGA
  CATCCGCGCCCTCTCCGCTGCGCCTCATGGATCTACTCTCCAAGAAGCACCCGCTGGACACTCTGTTCCT
  GCTGGCCGGGCCAGACACAGTGCTCACACCCGACTTCCTGAACCGCTGCCGCATGCACGCCATCTCGGGC
  TGGCAAGCCTTCTTTCCCATGCACTTCCAGGCCTTCCACCCTGCTGTGGCTCCACCTCAGGGCCCTGGGC
  CACCAGAACTGGGCCGTGACACCGGCCACTTTGATCGTCAGGCTGCCAGCGAGGCATGCTTCTACAACTC
  CGACTATGTGGCGGCCCGTGGACGGCTGGTGGCAGTCTCCGAGCAGGAGGAGGAGCTGCTGGAGAGCCTG
  GATGTGTATGAGTTGTTTCTACGCTTCTCTAGCTTGCATGTGCTGAGGGCAGTGGAACCAGCCTTGCTGC
  AGCGCTACCGGGCCCAGCCATGCAGCGCTCGGCTCAGCGAGGACCTTTATCACCGCTGTCGCCAGAGCGT
  GCTTGAGGGCCTTGGCTCCCGCACCCAGCTTGCCATGCTGCTCTTTGAACAGGAACAGGGTAACAGCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209665 representing NM_001005906
  Red=Cloning site Green=Tags(s)
  
  MRASLLLSVLRPAGPVAVGISLGFTLSLLSVTWVEEPCGPGPPQPGDSELPPRGNTNAARRPNSVQPGAE
  RERPGADESWEPRVLPYHPAQPGQATKKAVRTRYISTELGIRQKLLVAVLTSQATLPTLGVAVNRTLGHR
  LEHVVFLTGARGRRTPSGMAVVALGEERPIGHLHLALRHLLEQHGDDFDWFFLVPDATYTEAHGLDRLTG
  HLSLASATHLYLGRPQDFIGGETTPGRYCHGGFGVLLSRTLLQQLRPHLESCRNDIVSARPDEWLGRCIL
  DATGIGCTGHHEGMHYNYLELSPGEPVQEGDPRFRSALTAHPVHDPVHMYQLHKAFARAELDRTYQEIQE
  LQWEIQNTSRLAADGERASTWPVGIPAPSRPASRFEVLRWDYFTEQHTFSCADGSPRCLLRGADQADVAD
  VLGTALEELNRRYQPALRLQKQQLVNGYRRFDPARGMEYTLDLQLEALTPQGGRWPLTHRVQLLRPLSRV
  EILPVPYVTEASRVTVLLPLAAAECDLASGFLEAFATAALEPGDAAALTLLLLYEPRQAQRAAHADVFAP
  VKAHVAELERRFPGARVPWLSVQTSAPSPLRLMDLLSKKHPLDTLFLLAGPDTVLTPDFLNRCRMHAISG
  WQAFFPMHFQAFHPAVAPPQGPGPPELGRDTGHFDRQAASEACFYNSDYVAARGRLVAVSEQEEELLESL
  DVYELFLRFSSLHVLRAVEPALLQRYRAQPCSARLSEDLYHRCRQSVLEGLGSRTQLAMLLFEQEQGNST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005906
• ORF Size: 2310 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005906.1, NP_001005906.1
• RefSeq Size: 2946 bp
• RefSeq ORF: 2313 bp
• Locus ID: 316533
• Cytogenetics: 9q33
• MW: 85.3 kDa