Eif5 (NM_020075) Rat Tagged ORF Clone

CAT#: RR209558

Eif5 (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 5 (Eif5), (10 ug)

Product Images

Circular map for RR209558

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209558 representing NM_020075
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTCAACGTCAACCGCAGCGTGTCAGACCAGTTCTATCGCTACAAGATGCCCCGTTTGATTGCTA
  AGGTTGAGGGCAAAGGAAATGGAATCAAGACAGTTATAGTCAACATGGTTGACGTTGCAAAGGCGCTTAA
  TCGGCCTCCAACGTATCCCACCAAATATTTTGGTTGTGAGCTGGGAGCACAGACCCAGTTTGATGTTAAG
  AATGACCGTTACATTGTCAATGGATCTCATGAGGCGAATAAGCTGCAAGACATGTTGGATGGATTCATTA
  AAAAATTTGTTCTCTGTCCTGAGTGTGAGAATCCTGAAACAGATCTGCATGTCAATCCAAAGAAGCAAAC
  AATAGGTAATTCTTGTAAAGCCTGTGGGTACCGAGGCATGCTTGACACACATCATAAACTCTGTACATTC
  ATTCTCAAAAACCCACCTGAGAATAGTGACATTGGTACAGGAAAGAAAGAGAAAGAAAAGAAAAATAGAA
  AGGGCAAGGACAAGGAAAATGGCTCTGTATCCACCAGTGAGACACCACCACCTCCACCACCAAATGAAAT
  TAGTCCTCCACATGCTGTGGAAGAAGAGGAAGATGATGATTGGGGGGAGGATACAACTGAGGAAGCTCAA
  AGGCGCAGAATGGATGAAATCAGTGACCATGCAAAAGGTCTGACACTTAGCGATGATTTGGAAAGAACTG
  TAGAAGAGCGTGTTAACATCCTGTTTGATTTTGTTAAGAAAAAGAAAGAAGAGGGCATTATTGATTCATC
  TGATAAAGACATTGTGGCTGAGGCAGAAAGACTGGATGTAAAAGCCATGGGCCCTCTCGTTTTGACAGAA
  GTTCTCTTTGATGAGAAGATAAGAGAGCAAATCAAGAAATACAGGCGCCATTTTTTAAGATTTTGTCATA
  ACAACAAAAAGGCCCAGCGGTACCTTCTTCATGGTTTGGAATGTGTGGTAGCAATGCATCAAGCTCAGTT
  GATCTCCAAGATTCCACATATCTTGAAGGAGATGTATGATGCAGACCTTTTAGAGGAAGAGGTCATTATC
  AGCTGGTCAGAAAAGGCCTCTAAGAAATATGTCTCAAAAGAACTTGCCAAAGAGATTCGTGTCAAAGCAG
  AGCCATTTATTAAATGGTTGAAGGAAGCGGAGGAGGAATCTTCTGGTGGTGAGGAAGAAGACGAAGATGA
  AAATATTGAGGTGGTATATTCGAAGACTGCCAGTGTACCAAAAGTTGAAACTGTGAAGTCTGACAACAAG
  GATGACGACATTGATATTGATGCCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209558 representing NM_020075
  Red=Cloning site Green=Tags(s)
  
  MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVK
  NDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTF
  ILKNPPENSDIGTGKKEKEKKNRKGKDKENGSVSTSETPPPPPPNEISPPHAVEEEEDDDWGEDTTEEAQ
  RRRMDEISDHAKGLTLSDDLERTVEERVNILFDFVKKKKEEGIIDSSDKDIVAEAERLDVKAMGPLVLTE
  VLFDEKIREQIKKYRRHFLRFCHNNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVII
  SWSEKASKKYVSKELAKEIRVKAEPFIKWLKEAEEESSGGEEEDEDENIEVVYSKTASVPKVETVKSDNK
  DDDIDIDAI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020075
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020075.1, NP_064460.1
• RefSeq Size: 3504 bp
• RefSeq ORF: 1290 bp
• Locus ID: 56783
• UniProt ID: Q07205
• Cytogenetics: 6q32
• MW: 49 kDa
• Gene Summary: translation initiation factor that catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex [RGD, Feb 2006]