Dysf (NM_001107869) Rat Tagged ORF Clone

CAT#: RR209414

Dysf (Myc-DDK-tagged ORF) - Rat dysferlin (Dysf), (10 ug)

Product Images

Circular map for RR209414

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dysf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209414 representing NM_001107869
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTGCTGCCTGCTGGCAAGGGCCAGCAACCTCCCCAATGTGAAGAAGGACCGGCGCAGTGACCCTG
  TTGCCAGCCTGATTTTTCGAGAAGCCAAGATCCCACTCCAGGAGGTCCTTGCCACCCCCAGCCTATCTGC
  CAGCTTCAACGCACCTCTGCTGGACTCCAAGCAGCAACCCACAGGGGCCTCTCTGGTCCTGCAGGTATCC
  TACACACCGCCCCCAGGAGCTGCGCCCTTGTTCCCACCACCCGCTTCCCTGGCACCGTCCCCAACACTGC
  CTGACATGGACCTGGTGGCTGACACAGGAGGAGAGGAAGACACGGAAGACCAGGGCCTGACTGGAGATGA
  AGCTGAGCTCTTCCCAGATCCGAGTGTTGCTGTGGGCCCAGGGGGACCAACGACGCCCAAGAAGCCACCT
  TCCCATCCTCCTCCCCACTATCCAGGGGGGAAAAGGAAAAGAAGCTCTGCTCCACCTAGAAAGCTGCTGT
  CAGACAAGCCACAGGACTTCCAGATCAGAGTCCAGGTGATTGAGGGGCGTCAGCTGCCTGGGGTGAACAT
  CAAGCCTGTGGTCAAGGTCACAGCTGCTGGGCAGACCAAGCGGACTCGAATCCAGAAGGGAAATAGCCCA
  CTTTTCAATGAGACTCTTTTCTTCAACGTATTTGACTCTCCCTTGGAACTGTTTGATGAGCCCATCTTCA
  TCACGGTGGTAGACTCACGCTCCCTCAGGACAGATGCCCTCCTTGGCGAGTTCCGGATGGATGTGGGCAC
  CGTGTACAGAGAGCCCCGACATGCTTATCTCCGGAAGTGGCTGCTGCTCTCCGATCCCGATGACTTCTCT
  GCAGGGGCCAGAGGCTACCTCAAAGCCAGCCTGTGTGTGCTGGGGCCTGGGGACGAAGCTCCCCTAGACA
  AGAAAGACCCTTCTGAAGACAAAGAGGACATTGAAGGCAACCTGCTTAGGCCCACTGGTGTGGCCCTTCG
  TGGTGCCCATTTTTGCCTGAAGCTCTTCCGGGCTGAGGACTTACCACAGATGGACGATGCTGTGATGGAC
  AACGTCAAGCAAATCTTTGGCTTTGATAGCAACAAGAAGAACCTGGTGGACCCCTTTGTGGAAGTCAGCT
  TTGCTGGGAAAATGCTTTGCAGCAAGATCCTGGAGAAGACTGCTAACCCTCAGTGGAACCAGAACATCAC
  ATTGCCTGCGATGTTTCCCTCTATGTGTGAAAAAATGAGGATTCGTGTCATGGACTGGGACCGCCTCACT
  CACAATGACACTGTGGCTACCACCTACTTGGGTATGTCGAAAATCTCTGCCACTGGAGGAGAAATAGAAG
  AGGAGCCTGCAGGTGTACTCAAGAGTCCAGCCACTGACCTGGATGACAACCTGGGCTTCCTCCCCACTTT
  TGGGCCCTGCTATGTGAACCTCTATGGCAGTCCCCGAGAGTTCACGGGCTTCCCAGATCCTTATGCGGAG
  CTCAACACAGGAAAGGGAGAAGGTGTGGCATATAGGGGCAGGCTTCTGTTGTCCCTGGAGACCAAACTGG
  TGGAGCACAGTGAACAGAAGGTGGAAGACCTTCCTGCTGATGACATCTTGAGAGTGGAGAAGTACCTGCG
  GAGGCGCAAATATTCCCTCTTTGCTGCCTTCTACTCAGCCACAATGCTGCAGGATGTGGATGATGCCATC
  CAGTTCGAGGTCAGCATTGGGAACTACGGAAACAAGTTTGACACCACCTGCTTGCCGTTGGCCTCTACCA
  CCCAATACAGCCGGGCAGTCTTTGATGGATGCCACTACTATTATTTGCCCTGGGGCAACGTGAAGCCTGT
  GGTAGTACTGTCCTCATACTGGGAAGACATCAGCCATCGAGTCGAGACTCAGAACCAGCTCCTCAGGATC
  GCTGACCGACTGGAAGCTAACCTGGAGCAAGTCCACCTGGCTCTGAAGGCACAGTGTTCCTCTGAGGATG
  TGGACGCCCTGGTGGCTCAGTTGACAGATGAGCTCCTTGCAGACTGCAGCCAGCCCCTGTGTGACATCCA
  TGAAATACCTTCTGCCACCCACCTTGATCAGTACCTGTTCCGCCTCCGCTCCCGTCACCTGAGCCAGATC
  AAAGAAGCTGCCCTGGCCTTGAAGCTTGGCCACAGTGAGCTCTCCACTGCCCTGGAGCAGGCCGAGGACT
  GGCTTCTGCATCTCCGTGCCCTGGCTGAGGAGCCCCAGAACAGCCTGCCAGATATCATCATCTGGATGCT
  GCAGGGTGACAAGCGTGTGGCCTACCAACGGGTGCCTGCCCATGAAGTCCTCTTCTCCCGGCGGGGCCCC
  AGCTACTGCGGCAGGAACTGTGGGAATCTGCAGACCATCTTTCTGAAATATCCTATGGAAGGCATGCCTG
  GGCCCCGGATGCCGGTGCAGATCCGGATCAAGCTCTGGTTTGGGCTCTCAGTGGATGAGAAGGAATTCAA
  TCAGTTTGCAGAGGGGAAGCTCTCTGTCTTTGCTGAAACGTATGAGAACCAGACAAAGCTGGCGCTGGTC
  GGGAACTGGGGCACGACGGGCCTCACCTACCCCAAGTTTTCTGACGTCACAGGCAAGATCAAACTACCGA
  AGGATAGCTTCCGCCCCTCAGCTGGCTGGGCCTGGGCAGGAGACTGGTTCGTGTGTCCAGAGAAGACCCT
  GCTCCATGATGCTGACGCCGGTCATCTGAGCTTTGTGGAGGAGGTGTTTGAGAACCAGACTCGGCTACCT
  GGAGGCCAGTGGATCTATATGAGTGACAACTACACCGACGTGAATGGGGAGAAGGTGCTTCCAAAGGACG
  ATATTGAATGCCCGCTGGGTTGGAAGTGGGAAGATGAAGAATGGTCCACAGACCTCAATCGGGCTGTTGA
  TGAACAAGGCTGGGAGTACAGCATCACCATACCCCCAGACCGAAAGCCCAAACACTGGGTCCCTGTTGAG
  AAGATGTATTACACTCACAGGCGTCGACGCTGGGTCCGACTGCGCAGGAGGGACCTCAGCCAGATGGAGG
  CACTGAAGAAGCACAGGCAGGCAGAGGCTGAGGGCGAGGGCTGGGAATACGCCTCTCTCTTTGGCTGGAA
  GTTCCACCTGGAGTACCGCAAGACAGATGCCTTTCGCCGCCGCCGCTGGCGCCGCCGCATGGAGCCACTG
  GAGAAGACGGGGCCTGCAGCGGTGTTTGCCCTTGAGGGGGCCCTGGGCGGCATGGTGGATGACAAGAGCG
  AAGATTCCATGTCTGTGTCTACCCTGAGCTTTGGCGTTAACAGACCCACAATCTCCTGCATCTTTGACTA
  TGGGAACCGCTACCATCTGCGTTGCTACCTGTACCAGGCCCGGGACCTGCCCGCCATGGACAAAGACTCC
  TTCTCTGATCCCTATGCCATTGTCTCCTTCCTGCATCAAAGCCAGAAGACGGTGGTGGAGAAGAACACCC
  TGAACCCCACCTGGGATCAAACCCTCATCTTCTATGAGATAGAGATCTTTGGCGAGCCGGCCAGTGTCGC
  CGAGCGGCCACCCAGCATTGTGGTGGAGCTGTATGACCATGACACCTATGGCGCTGACGAGTTCATGGGC
  CGCTGTATTTGTCAGCCAAGCCTGGAACGGATGCCCCGCCTGGCCTGGTTCCCACTGACCAGGGGCAGCC
  AGCCTGCCGGAGAGCTGCTGGCAGCGTTCGAGCTTATCCAGAGAGAGAAGCCAGCCATCCATCACATCCC
  TGGCTTTGAGATGCACGAAACTTCAAGGATTTTGGATGAGACGGAGGACACAGACTTGCCTTACCCACCA
  CCTCAAAGGGAGGCCAACATCTACATGGTTCCCCAGAACATCAAGCCAGCTCTCCAGCGTACAGCCATCG
  AGATCCTGGCCTGGGGCCTTCGGAACATAAAGAGCTATCAGATGGCCAGCGTGTCTTCCCCCAGCCTCGT
  GGTGGAGTGTGGTGGGCAGACAGTGCAGTCCTGTGTCATCAGAAACCTTCGGAAGAACCCCAACTTTGAC
  GTCTGTACTCTGTTCATGGAAGTGATGTTACCCAGAGAGGACCTCTACTGTCCCCCTATTGTGGTCAAGG
  TCATTGATAATCGCCAGTTTGGCCGCAGGCCAGTTGTGGGTCAGTGCACCATTCGCTCCCTGGAGAACTT
  CCTGTGTGACCCGTACTCAGCAGAGAGTCCATCCCCGCAAGGTGGCCCAGATGACGTGAGCTTACTCAGC
  CCTGGGGAAGACGTGCTCATTGACATTGATGACAAGGAGCCTCTCATCCCTGTCCAGGAGGAGGAGTTCA
  TTGACTGGTGGAGCAAATTCTTTGCTTCTATCGGGGAGAGAGAAAAGTGTGGTTCCTACCTGGAGAAGGA
  TTTTGACACCCTGAAGGTCTATGACACACAGCTGGAGAATGTGGAGGCCTTTGGAGGCCTGTCCGACTTT
  TGTAATACCTTCAAGCTCTACCGGGGCAAGACTCAGGAGGACACAGAAGATCCATCTGTCATCGGAGAAT
  TTAAGGGTCTCTTCAAAATTTATCCCCTCCCAGAAGATCCAGCCATCCCTATGCCCCCGAGACAATTCCA
  TCAGCTGGCTGCTCAGGGTCCCCAGGAATGCTTGGTCCGGATTTACATCATCCGAGCATTTGGCTTACAG
  CCCAAGGACCCCAATGGCAAGTGCGACCCTTACATCAAGATCTCTATAGGGAAGAAATCAGTGAGTGACC
  AGGATAACTACATCCCTTGTACCCTGGAGCCTGTATTTGGAAAGATGTTTGAACTCACCTGCACTCTACC
  TCTGGAGAAGGACTTGAAGATCACACTCTATGACTATGACCTGCTCTCCAAGGATGAGAAGATTGGGGAG
  ACAGTCATTGATCTGGAGAATAGGCTGCTGTCCAAGTTTGGGGCTCGCTGTGGCCTTCCACAGACCTACT
  GTGTCTCTGGACCAAATCAGTGGAGAGATCAACTCCGCCCTTCCCAGCTCCTCCACCTCTTCTGCCAGCA
  GCACAGGGTCAAGGCCCCCGTGTACCGGACAGACCGAGTGACGTTTCAGGATAAGGAATACACCATTCAG
  GAGATAGAGGCTGGCAGACTCCCAAACCCACACCTGGGCCCGGTGGAGGAACGCTTAGCCCTGCATGTCC
  TTCAACAACAAGGCTTGGTCCCTGAGCATGTGGAGTCTCGGCCTCTGTACAGTCCTCTGCAGCCAGATAT
  TGAGCAGGGGAAGCTACAGATGTGGATTGACATATTTCCAAAGGTGCTGGGCCGGCCTGGACCTCCCTTC
  AACATCACCCCACGGAAAGCTCGAAGGTTTTTTCTGCGTTGTATTATTTGGAACACAAAAGATGTGATCT
  TGGATGACCTCAGCCTCACGGGGGAGAAGATGAGTGACATCTATGTGAAAGGCTGGATGGTGGGATTCGA
  AGAACACAAACAGAAGACAGATGTGCACTACCGTTCCCTGGGCGGGGAGGGCAATTTCAACTGGAGGTTC
  GTGTTTCCCTTCGACTATCTGCCCGCTGAGCAAGTCTGTGCTGTTGCCAAGAAGGATGCTTTCTGGAGAC
  TCGACAAGACAGAGAGCAAGATCCCAGCACGTGTGGTCTTCCAGATTTGGGACAATGACAAGTTCTCCTT
  TGATGACTTTCTGGGCTCCCTGCAGCTGGATCTCAACCGAATGCCCAAGCCAGCCAAGACGGCTGAGAAG
  TGCTCCTTGGACCAGCTGGATGACACCTTCCACCCAGAATGGTTTGTGTCCCTTTTTGAGCAGAAGACAG
  TGAAAGGATGGTGGCCTTGTGTGACAGAGGAGGGCGCGAAGAAGATCCTGGCGGGGAAGCTGGAAATGAC
  CTTGGAGATCGTTGCAGAAAGTGAACATGAGGAGCGGCCTGCTGGCCAGGGTCGGGATGAACCCAACATG
  AATCCAAAGCTAGAAGATCCCAGGCGCCCTGACACCTCTTTCCTGTGGTTCACCTCACCATACAAGACCA
  TGAAGTTCATCTTGTGGCGACGATTCCGGTGTGCCATCATCCTCTTCATCATCCTCTTCATCCTCCTGCT
  CTTCCTGGGTGTCTTTGTCTACGCCTTCCCGAACTATGCTGCCATGAAGCTGGTGAAGCCCTTTAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209414 representing NM_001107869
  Red=Cloning site Green=Tags(s)
  
  MLCCLLARASNLPNVKKDRRSDPVASLIFREAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVS
  YTPPPGAAPLFPPPASLAPSPTLPDMDLVADTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPP
  SHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSP
  LFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFS
  AGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMD
  NVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLT
  HNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAE
  LNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAI
  QFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRI
  ADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQI
  KEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGP
  SYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALV
  GNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLP
  GGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVE
  KMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPL
  EKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDS
  FSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMG
  RCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPP
  PQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFD
  VCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLS
  PGEDVLIDIDDKEPLIPVQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDF
  CNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQ
  PKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGE
  TVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQ
  EIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPF
  NITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRF
  VFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEK
  CSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNM
  NPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107869
• ORF Size: 6087 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107869.1, NP_001101339.1
• RefSeq Size: 6319 bp
• RefSeq ORF: 6090 bp
• Locus ID: 312492
• Cytogenetics: 4q34
• MW: 231 kDa