Fgd6 (NM_001137645) Rat Tagged ORF Clone

CAT#: RR209254

Fgd6 (Myc-DDK-tagged ORF) - Rat FYVE, RhoGEF and PH domain containing 6 (Fgd6), (10 ug)

Product Images

Circular map for RR209254

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fgd6
• Synonyms: RGD1565609
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209254 representing NM_001137645
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCTGCCGTGGAGCTGAAGAAGCCACCATTGGCTCCCAAGCCTAAGTTAGTGGGGACCAATAATA
  AGTCACCGCCTCCTCCTATTGCCCCTAAACCGGACATTGGAAATGCTAGTGTTCCACGGTTGACGAAGAC
  CAAACCGGCAATTGCTCCAAAACCGAAAGTCCTGACAAACTCAGATGTTCAAGACGTCAAGGATCAACCC
  TCAAAGAAGCTCACTTTGAACCTGGAGGAGCGGGAGCCAGAATTACCCGAGAGCACTGGCAAGTTTAATT
  GCAAAGATGTCGGGGATGCACACGGTGATTATATTTTCCCAACATGTTCTTGCAGTTCTGGGTGTACTCA
  TGAGCCTAGGACTCGCGAAAATCTGTGTGCAGAGCAGCTTGTTTTAGAGCCTCTGGGAATGAAAGAACAT
  TTAGAGAATGGTAAAAATGGTGAGTCCTCAAAGAGGGGGAGCAGATGGAATTCAAGCCGTGAAAAGTGCC
  GGAGCCAAAGTGGAGTTGTTTTAAAGGCAAGCATCTTCGAAGAGAAGCTTAAAGAGGTTCTCACGCAGCC
  GAGATCAAGGTCACCTAGTAGTTCCCCAAGGAAGCACAGATGTCCAAATAAACCAGAAATGAATGGCGAC
  CACAGTTGCGCCAGACAGGTCAGAATTGAGTTTGCAGACACCTCTTCTTCCCAGTCCAGCTTCGGAAAAG
  CTCCTCACAGCTGCCACACACAACCTCCCAGGGACAAATCTCAGAATCTCCAGACTTGTCAAGATGGCAG
  TGCTGAGAGCCTTGGTCCCTTTCATTCATGTGAGCTGGAGGGCAAAAGAGGGTGTTCCGGCGGAACTAGT
  CAGAAGTCGGAGGTCCAAGGTCTCGGTCCTGTAGAAATCCACTTACTACCGTATACCTCAAAGTTTCCAA
  CGCCCAAGCCCAGAAGGACACACGCTGCTCGTCTGCGTCGCCAGAAGCATGTAGACACTCCTAGTGAAAG
  CACTGAAGAGCCAGAGAGCTCAAACAATGGCTCTTCTTGTCTCCATGAAGATAGTTTGAAAAACAATAAA
  GTCAGTGTTCTTCATCAGAATGCTTTGTCTAACCAGGGACCAGTGGATGAGGTGAGGCCAGGAAACAAGA
  AGGCATTGACCAGGGAGTCCAACAGTGACAGACAGGACTCAGTCAGCTCACAGAAAGCTGTGCAGCATGA
  AACGTCTTCTTTTGAAAAAGTGGCACCTTCTTTAGATACAGATTCTAGTTTGACTTCTGACAGCACGGTA
  GAAGACGGTTCTGATGTGTTACCTGCTGTGGACAGAGAGACTGCTTTCATACAATGCAGTCCTCAGCCTT
  TGAGCTTGCCTAAGCAAGTCAAATTAGCCTGTGCTGAACAGCAGCCAACTACCTGCAACAGGGAGGTGTC
  AGTGCCCCAGATACAAAAGGAATCTTCCTCGAGAATTGTTCCCAAAAAACCTCAAAGACACAGCTTGCCT
  GCGGCAGGGGTGCTTAAAAAGGCTGCATCAGAGGAACTAGTAGAAAAGAACTCTTCAAGTAAGGAAACCA
  ATTCAGAGAAAGGTCTGCAAAGAAACCACCTTCAACATTTGGGTCCCTCAAACCATGGCATGTCACCGTC
  ATCCTTTGATATGCCCAACCCAACTTCAGAAAAGCCAGTATGGAAGTTACCTCATCCTATTCTACCTTTT
  CCGGGGAGCCCAGAAGCCTTGAAGCGGGTCACTTTATCCTTAAATAATGAGCCTTCCATTTCCCTAACCA
  AGCCAAGAGCAAAATCCTTGTCTGCTGTGGATATGGACAGGTGCAGTAAGCCTTGCAAAGAGCCTCCAAA
  GAAAACGACTTTTAAAAAGTTGATTAACGTGAAGCTGTCCATTGGTTTCATAAAGAGTGATTTTCACAAA
  TTTAAGTCAAAAAACTGCCAACATGGGGATGTCTCTACCGGACATTCGCTCGGTAGAGAGCCAAAAGGGC
  TTGAAAATAATTGGCAAGGTTTGGCCACAGGAGAAGAGAAGAAAAGTAAACCCATAAAGGCATATTCTGA
  AGAAAACTGCAGCCTGGAATCTCAAAAGGTGAAATCTTGGAGCCAGAGCAGTGCAACTAATGGTCAGAGA
  GCGGAGTCTTTGGATGACCAGATGCTCTCCCGGCACGCATCCTGTCAGGCCTCTAGTACAAGTGACTGTG
  GGCCAGAGTATGAAAATGTCCGCCATTATGAGGAGATACCAGAGTATGAGAACTTGCCTTTTGTCATGGC
  TCGAGGGAACCCTCCAGAATTGGGATGGCAGAATTCCAGCAGTGTGGAGGACACGGATGTAAGTCTGTAT
  GAAGTGGAAGAGCCCTATGACGCTCCAGATGGCCAGCTGCAACTTGATCCGAGACATCAGCCTTGCAGTT
  CTGGAAGATGCCAGGACAGGCAGGATGAGCTCCATCTTGACCTGCCTTCTGATGAGGAAGTCATCAATAG
  TTCTGACGAAGATGACGTCAGCTCCGAGTCCAGTAAAGGCGAGCCGGACCCGCTGGAGGATAAACAGGAT
  GAAGATGCTGGGATGAAAAGCAAAGTCCATCATATTGCCAAGGAGATCATGAGCTCGGAGAAAGTGTTTG
  TCGATGTGTTAAAACTTCTGCATATTGATTTCCGGGGTGCTGTAGCCCACGCGTCCAGGCAACTCGGGAA
  GCCCGTGATTGAGGACCGGATCCTTAACCAGATCCTGTACTACCTGCCTCAGCTGTATGAGCTCAACCGG
  GATCTCCTGAAGGAACTGGAGGAAAGAATGCTGAGCTGGGCTGAACAACAGAGAATTGCTGACATCTTTG
  TAAAGAAGGGGCCATATTTAAAAATGTACTCCATGTACATCAAAGAATTCGACAAGAACATTGCGTTGCT
  GGATGAACAGTGCAAGAAAAACTCTGGATTCGCCACGGTCGTCAGGGAATTCGAGATGAGCCCTCGATGT
  GCCAACCTGGCCCTGAAGCACTACCTGCTGAAGCCAGTTCAGAGGATCCCTCAGTATAGGCTGCTGCTGA
  CAGATTATTTAAAGAACCTCCTAGAAGACTCTGTAGACCACAGAGACACCCAAGATGCCCTAGCTGTTGT
  CATAGAGGTAGCTAACCATGCCAATGACACCATGAAGCAAGGGGACAACTTTCAGAAGCTCATGCAAATT
  CAGTACAGCTTGAGTGGACACCATGAAATCGTGCAGCCTGGGCGGGTGTTTCTTAAAGAAGGCATCCTGA
  TGAAGCTGTCTCGGAAAGTCATGCAGCCGCGAATGATTTTCCTGTTTAATGATGCTCTACTGTACACCAC
  ACCCATGCAGTCTGGGATGTACAAACTGAACAACATGCTCTCCTTGGCTGGAATGAAGGTCAGAAAACCT
  ACCCAGGAGGCATATCAGAACGAGTTAAAGATTGAAAGTGTAGAGCGTTCTTTCATCCTCTCAGCAAGTT
  CTGCCTCAGAAAGAGACGACTGGCTGGAGGCCATATCCAGGGCTATAGAAGAGTATGCCAAGAAAAGAAT
  CACCTTCTGCCCCAGCAGGAGTCTTGATGAGGACTCAGAAAGGAAAGAAGAAGTCAGCCCTCTTGGGGCA
  AAGGCTCCCATCTGGATTCCTGACACCAGAGCCACCATGTGTATGATTTGCACAAGTGAATTCACCCTGA
  CCTGGAGAAGACACCACTGCAGGGCCTGTGGGAAGATCGTGTGCCAAGCTTGCTCGTCCAACAAGTGTGG
  CTTGGATTACCTGAAAGGGCAGCCGGCGAGAGTGTGCGAGCTCTGCTTCCAAGAGCTGCAGAAACTAGAT
  CACCAGCTGTCCCCCAGGATTGGGTCTCCTGGAAATCACAAGTCTCCTTCAAGTGCCTTATCTTCAGTCT
  TACATAGCATCCCCTCAGGGAGGAAACAGAAGAAAATCCCTGCTGCCCTCAAAGAAGTGTCAGCGAACAC
  AGAGGACTCCACCATGAGCGGCTACCTCTACAGATCCAAGGGCAGTAAAAAGCCGTGGAAACACCTATGG
  TTCGTCATCAAGAACAAAGTGCTCTACACATACGCTGCAAGCGAGGATGTGGCAGCTTTGGAAAGTCAGC
  CCTTATTGGGATTCACCGTCGCTCAAGTCAAAGACGAGCACTCGGATCCTAGAGTGTTTCAGCTGCTGCA
  CAAAGGCTTGCTGTTCTACGTGTTCAAGGCAGACGACGCCCACTCCACTCAGAGGTGGATAGACGCATTT
  CAGGAGGGCACAGTATTA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209254 representing NM_001137645
  Red=Cloning site Green=Tags(s)
  
  MTSAVELKKPPLAPKPKLVGTNNKSPPPPIAPKPDIGNASVPRLTKTKPAIAPKPKVLTNSDVQDVKDQP
  SKKLTLNLEEREPELPESTGKFNCKDVGDAHGDYIFPTCSCSSGCTHEPRTRENLCAEQLVLEPLGMKEH
  LENGKNGESSKRGSRWNSSREKCRSQSGVVLKASIFEEKLKEVLTQPRSRSPSSSPRKHRCPNKPEMNGD
  HSCARQVRIEFADTSSSQSSFGKAPHSCHTQPPRDKSQNLQTCQDGSAESLGPFHSCELEGKRGCSGGTS
  QKSEVQGLGPVEIHLLPYTSKFPTPKPRRTHAARLRRQKHVDTPSESTEEPESSNNGSSCLHEDSLKNNK
  VSVLHQNALSNQGPVDEVRPGNKKALTRESNSDRQDSVSSQKAVQHETSSFEKVAPSLDTDSSLTSDSTV
  EDGSDVLPAVDRETAFIQCSPQPLSLPKQVKLACAEQQPTTCNREVSVPQIQKESSSRIVPKKPQRHSLP
  AAGVLKKAASEELVEKNSSSKETNSEKGLQRNHLQHLGPSNHGMSPSSFDMPNPTSEKPVWKLPHPILPF
  PGSPEALKRVTLSLNNEPSISLTKPRAKSLSAVDMDRCSKPCKEPPKKTTFKKLINVKLSIGFIKSDFHK
  FKSKNCQHGDVSTGHSLGREPKGLENNWQGLATGEEKKSKPIKAYSEENCSLESQKVKSWSQSSATNGQR
  AESLDDQMLSRHASCQASSTSDCGPEYENVRHYEEIPEYENLPFVMARGNPPELGWQNSSSVEDTDVSLY
  EVEEPYDAPDGQLQLDPRHQPCSSGRCQDRQDELHLDLPSDEEVINSSDEDDVSSESSKGEPDPLEDKQD
  EDAGMKSKVHHIAKEIMSSEKVFVDVLKLLHIDFRGAVAHASRQLGKPVIEDRILNQILYYLPQLYELNR
  DLLKELEERMLSWAEQQRIADIFVKKGPYLKMYSMYIKEFDKNIALLDEQCKKNSGFATVVREFEMSPRC
  ANLALKHYLLKPVQRIPQYRLLLTDYLKNLLEDSVDHRDTQDALAVVIEVANHANDTMKQGDNFQKLMQI
  QYSLSGHHEIVQPGRVFLKEGILMKLSRKVMQPRMIFLFNDALLYTTPMQSGMYKLNNMLSLAGMKVRKP
  TQEAYQNELKIESVERSFILSASSASERDDWLEAISRAIEEYAKKRITFCPSRSLDEDSERKEEVSPLGA
  KAPIWIPDTRATMCMICTSEFTLTWRRHHCRACGKIVCQACSSNKCGLDYLKGQPARVCELCFQELQKLD
  HQLSPRIGSPGNHKSPSSALSSVLHSIPSGRKQKKIPAALKEVSANTEDSTMSGYLYRSKGSKKPWKHLW
  FVIKNKVLYTYAASEDVAALESQPLLGFTVAQVKDEHSDPRVFQLLHKGLLFYVFKADDAHSTQRWIDAF
  QEGTVL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001137645
• ORF Size: 4218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001137645.1, NP_001131117.1
• RefSeq Size: 4732 bp
• RefSeq ORF: 4221 bp
• Locus ID: 500824
• Cytogenetics: 7q13
• MW: 157 kDa