Chst3 (NM_053408) Rat Tagged ORF Clone

CAT#: RR208913

Chst3 (Myc-DDK-tagged ORF) - Rat carbohydrate sulfotransferase 3 (Chst3), (10 ug)

Product Images

Circular map for RR208913

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chst3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208913 representing NM_053408
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAAGGACTCGCTTTGCCTCAGGATTGCCGGGACCTTGTGCACAACCTGAAGATTCGAGGCAGAT
  ATGTCCTGTTCTTGGCATTTGTGGTCATAGTTTTTATCTTCATTGAAAAGGAAAATAAAATCATATCCAG
  GGTCTCCGACAAGCTGAAGCAGATCCCTCAGTTTGTGGCAGATGCCAACAGCACCGACCCAGCCCTGCTC
  TTATCGGAAAATGCATCTCTCTTGTCCCTGAGCGAGCTGGATTCCACCTTCTCCCATCTGCGGAGCCGCC
  TGCACAACTTGAGCCTGCAGCTGGGCATAGCGCCAGCAATGGAGGCCCAGGGCCAGGAGGCTGTGGCAGA
  GAAGCCATCCCAGCAGGCTGGAGCAGGGACCCGGCGCCACGTGCTCCTCATGGCCACCACTCGCACCGGC
  TCCTCATTCGTGGGCGAGTTCTTCAACCAGCAAGGCAATATCTTTTACCTCTTTGAGCCACTGTGGCACA
  TCGAGCGCACCGTGTTCTTCCAGCAGGGAGGTGCCAGCGCGGCGGGCTCAGCGTTGGTCTACCGCGATGT
  CCTCAAGCAGCTATTGCTGTGTGACCTGTATGTGCTAGAGCCCTTCATCAGCCCTCCGCCCGAGGACCAC
  TTGACTCAGTTCCTGTTTCGCCGGGGATCCAGCCGCTCGCTCTGCGAGGATCCGGTGTGCACACCCTTCG
  TCAAGAAGGTCTTCGAGAAGTACCACTGCAGGAACCGTCATTGCGGGCCACTCAACGTGACCTTGGCCGC
  CGAGGCCTGCCGTCGCAAGGACCACATGGCCCTCAAGGTCGTGCGCATCCGTCAGCTGGAGTTCCTGCAG
  CCTCTGGCTGAGGACCCAAGGCTGGACCTGCGAGTCATCCAGCTGGTGCGCGACCCCCGGGCCGTGCTGG
  CTTCACGCATGGTGGCCTTTGCGGGCAAGTATGAGAGCTGGAAGAAGTGGCTGTCCGAGGGCCAGGACCA
  GCTGAGCGAGAATGAGGTGCAGCGGTTGCGGGGCAACTGTGAGAACATCCGCCTGTCTGCAGAGCTGGGC
  TTGCGGCAGCCAGCCTGGCTGCGCGGTCGGTACATGCTGGTGCGCTACGAGGATGTGGCACGCAGGCCAC
  TGCAGAAGGCCCGAGAGATGTATCGCTCCGCAGGCATCCCCTTGACCCCGCAGGTGGAGGACTGGATCCA
  GAAGAACACGCAGGCGGCGCGCGACAGCAGCGACGTCTACTCCACTCAGAAGAACTCCTCGGAGCAGTTT
  GAGAAGTGGCGCTTCAGCATACCCTTCAAGCTGGCACAGGTGGTGCAGGCTGTCTGCGGCCCGGCCATGC
  ACCTCTTCGGCCACAAGTTGGCAAAGGATACCGCCTCACTCACCAACCGCTCCATCAGCCTGCTGGAGGA
  GCGGGGCACCTTCTGGGTCACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208913 representing NM_053408
  Red=Cloning site Green=Tags(s)
  
  MEKGLALPQDCRDLVHNLKIRGRYVLFLAFVVIVFIFIEKENKIISRVSDKLKQIPQFVADANSTDPALL
  LSENASLLSLSELDSTFSHLRSRLHNLSLQLGIAPAMEAQGQEAVAEKPSQQAGAGTRRHVLLMATTRTG
  SSFVGEFFNQQGNIFYLFEPLWHIERTVFFQQGGASAAGSALVYRDVLKQLLLCDLYVLEPFISPPPEDH
  LTQFLFRRGSSRSLCEDPVCTPFVKKVFEKYHCRNRHCGPLNVTLAAEACRRKDHMALKVVRIRQLEFLQ
  PLAEDPRLDLRVIQLVRDPRAVLASRMVAFAGKYESWKKWLSEGQDQLSENEVQRLRGNCENIRLSAELG
  LRQPAWLRGRYMLVRYEDVARRPLQKAREMYRSAGIPLTPQVEDWIQKNTQAARDSSDVYSTQKNSSEQF
  EKWRFSIPFKLAQVVQAVCGPAMHLFGHKLAKDTASLTNRSISLLEERGTFWVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053408
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053408.1, NP_445860.1
• RefSeq Size: 1425 bp
• RefSeq ORF: 1425 bp
• Locus ID: 84468
• UniProt ID: Q9QZL2
• Cytogenetics: 20q11
• MW: 54 kDa
• Gene Summary: mouse homolog is a transferase that catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to the N-acetylgalactosamine residue of chondroitin [RGD, Feb 2006]