Prkaa2 (NM_023991) Rat Tagged ORF Clone

CAT#: RR208871

Prkaa2 (Myc-DDK-tagged ORF) - Rat protein kinase, AMP-activated, alpha 2 catalytic subunit (Prkaa2), (10 ug)

Product Images

Circular map for RR208871

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prkaa2
• Synonyms: Ampk; Ampka2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208871 representing NM_023991
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGAAGCAGAAGCACGACGGGCGTGTGAAGATCGGACACTACGTGCTGGGGGACACCCTGGGCG
  TCGGCACCTTCGGCAAAGTGAAGATTGGAGAACATCAATTGACAGGCCATAAAGTGGCAGTTAAGATCTT
  AAATAGACAGAAGATTCGCAGTTTAGATGTTGTTGGAAAAATAAAACGAGAAATTCAAAATCTTAAACTC
  TTTCGTCATCCTCATATTATCAAACTCTACCAAGTGATCAGCACTCCAACAGACTTTTTTATGGTAATGG
  AATATGTGTCTGGAGGTGAATTGTTCGACTACATCTGTAAACACGGGAGGGTTGAAGAGGTGGAAGCTCG
  CCGGCTCTTCCAGCAGATTCTGTCTGCCGTGGACTACTGTCACAGGCACATGGTTGTCCACAGGGACCTG
  AAGCCAGAGAACGTGTTGCTGGACGCCCAGATGAATGCTAAGATAGCTGACTTCGGACTCTCTAATATGA
  TGTCAGATGGTGAATTTCTACGAACTAGCTGTGGATCGCCAAATTATGCAGCACCGGAGGTCATCTCAGG
  AAGGCTGTATGCGGGTCCTGAGGTTGATATCTGGAGCTGTGGTGTTATCCTGTATGCCCTTCTCTGTGGC
  ACCCTCCCGTTCGACGATGAGCACGTGCCTACGCTCTTTAAGAAGATCCGAGGGGGTGTGTTCTACATCC
  CGGAGTATCTCAACCGTTCTATTGCCACTCTGCTGATGCACATGCTGCAGGTGGACCCCTTGAAGCGAGC
  AACTATCAAAGACATACGAGAGCATGAATGGTTTAAACAGGATTTGCCCAGTTACCTCTTTCCTGAAGAC
  CCCTCCTATGATGCTAACGTCATTGATGATGAGGCTGTGAAAGAAGTATGTGAAAAATTTGAGTGTACAG
  AATCAGAAGTGATGAACAGTTTATACAGTGGTGACCCTCAAGACCAGCTCGCAGTGGCTTATCATCTCAT
  CATTGACAATCGGAGAATAATGAACCAAGCCAGTGAGTTCTACCTCGCCTCCAGTCCTCCAACGGGTTCC
  TTCATGGACGATATGGCCATGCACATTCCCCCCGGCCTGAAACCACATCCTGAAAGGATGCCACCTCTCA
  TAGCAGACAGCCCCAAAGCACGCTGTCCACTGGATGCACTCAACACAACTAAGCCCAAATCTTTAGCTGT
  GAAAAAAGCCAAGTGGCACCTTGGGATCCGAAGCCAGAGCAAACCATACGACATTATGGCGGAGGTGTAC
  CGAGCTATGAAGCAGCTGGACTTTGAATGGAAGGTAGTGAATGCATACCATCTTCGAGTAAGAAGAAAAA
  ACCCAGTGACTGGCAATTACGTGAAAATGAGCTTACAGCTTTACCTGGTTGACAATCGGAGCTATCTTCT
  AGACTTTAAAAGCATCGATGATGAGGTGGTGGAGCAGAGGTCTGGTTCTTCAACACCTCAGCGCTCCTGT
  TCTGCTGCCGGCCTCCACAGACCTCGGTCAAGTGTCGATTCCAGCACAGCCGAGAACCATTCACTGTCTG
  GCTCTCTCACTGGTTCTTTGACTGGCAGCACTTTGTCCTCCGCTTCCCCGCGCCTGGGCAGTCATACCAT
  GGATTTTTTTGAAATGTGCGCCAGTCTTATCACTGCTTTAGCCCGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208871 representing NM_023991
  Red=Cloning site Green=Tags(s)
  
  MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAVKILNRQKIRSLDVVGKIKREIQNLKL
  FRHPHIIKLYQVISTPTDFFMVMEYVSGGELFDYICKHGRVEEVEARRLFQQILSAVDYCHRHMVVHRDL
  KPENVLLDAQMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCG
  TLPFDDEHVPTLFKKIRGGVFYIPEYLNRSIATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPED
  PSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPTGS
  FMDDMAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVY
  RAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSC
  SAAGLHRPRSSVDSSTAENHSLSGSLTGSLTGSTLSSASPRLGSHTMDFFEMCASLITALAR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023991
• ORF Size: 1656 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023991.1, NP_076481.1
• RefSeq Size: 2652 bp
• RefSeq ORF: 1659 bp
• Locus ID: 78975
• UniProt ID: Q09137
• Cytogenetics: 5q34
• MW: 62.3 kDa
• Gene Summary: protein kinase that phosphorylates and inhibits acetyl-CoA carboxylase (ACC); rate-limiting enzyme in malonyl-CoA synthesis [RGD, Feb 2006]