Cnksr3 (NM_001012061) Rat Tagged ORF Clone

CAT#: RR208491

Cnksr3 (Myc-DDK-tagged ORF) - Rat Cnksr family member 3 (Cnksr3), (10 ug)

Product Images

Circular map for RR208491

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cnksr3
• Synonyms: Magi1; Prp4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208491 representing NM_001012061
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGTGACCAAGTGGAGCCCCAAACAAGTGGTGGACTGGACTAGAGGGTTGGATGACTGCCTGC
  AGCCGTACGTCCACAAGTTTGAGCGGGAGAAGATAGATGGGGAGCAGTTGCTAAAGATCTCCCACCAGGA
  TCTCGAGGAACTGGGGGTCACGCGGATCGGACACCAAGAGCTGGTGTTGGAGGCTGTGGACCTTCTCTGC
  GCACTGAATTATGGCCTTGAAACTGATAACATGAAGAACTTGGTTCTGAAGCTGCGGGCATCCTCCCATA
  ATCTGCAGAATTACATAAGTAGCCGGCGGAAGAGCCCAGCCTACGATGGGAACACGTCCCGCAAGCCCCC
  CAATGAGTTCCTGACTTCTGTGGTAGAGCTCATTGGTGCTGCCAAGGCCCTGCTGGCTTGGCTGGATCGG
  GCTCCATTCACAGGGATCACGGATCTGTCGGTAACAAAGAACAAAATCATCCAACTGTGCCTGGACCTGA
  CCACAGCAGTCCAGAAGGATTGCCTCGTAGCAGAAATGGAGGATAAAGTTTTAAATGTGGTCAAGGTCTT
  GAATGGCATCTGTGACAAAATGATGCACTCTACTACGGACCCTGTGATGAGCCAGTGCGCATGCCTGGAG
  GAAGTTCACTTACCGAATGTTAGACCCGGGGAAGGCCTGGGCATGTACATCAAGTCAACCTATGATGGAC
  TGCATGTGATTACTGGAACCACAGAAAATTCTCCTGCAGACAGATCTCAAAAGATTCATGCTGGTGACGA
  GGTCATTCAAGTCAATCGGCAAACAGTGGTCGGGTGGCAGTTGAAGAATCTGGTGAAGAAGTTGAGAGAG
  AATCCCACAGGCGTGGTGCTGCTGCTGAAGAAGAGGCCCACGGGCTCATTCAGCTTCACTCCAGCCCCGC
  TGAAGAACCTGAGGTGGAAGCCACCCCTCGTGCAGACCTCACCCCCGCCCACCACAACCCAGTCCCCAGA
  GAGCACCATGGATGCCTCCCTGAAGAAGGAGAAGCCAGCCATCCTGGACCTATACATTCCTCCCCCACCT
  GCTGTTCCCTACTCTCCCCGGGATGAGAACGTGAGTTTCAGCTATAGAGGACACACTAAGAGTAAACAGC
  CACTGCCTGGGCGCAAGGGTTCCGAGTCCCCTAACTCCTTCCTGGACCAGGAGAGCCAGAGACGCAGATT
  CACCATCACAGACTCCGATCAGCTACCCGGGTACTCGGTGGGGACCAATATTCTGCCCATAAAAATGAGA
  GGGAAGACGCCGTCCTATGGCAAACCCCGGCCTCTGTCCATGCCTGCAGATGGGAACTGGATGGGGATTG
  TGGACCCTTTTGCTAAACCTCGAGGTCATGGGAGGAAAGGGGAAGACGCCCTGTGCCGGTACTTCAGCAA
  TGAGCGCATTACACCCATCATTGAAGAAAGTGCCTCTCCCGTGTACCGGTTCTCCAGACCCCTGACTGAG
  CGGCATCTGGTTCGAGGTGCGGACTACATCCGAGGGAGCAGGTGCTATATCAACTCTGATCTACACAGCA
  GCGCCACCATTCCATTCCAGGAGGAAGGGCCCAAGAAGAAGTCGGCCTCCTCATCGGCCAAGGCGTCTTC
  TGGGGAGCCGTCGCTGTTGGTCAGCTGGCTGACTCGCCTCAAGCTGTTGACTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208491 representing NM_001012061
  Red=Cloning site Green=Tags(s)
  
  MEPVTKWSPKQVVDWTRGLDDCLQPYVHKFEREKIDGEQLLKISHQDLEELGVTRIGHQELVLEAVDLLC
  ALNYGLETDNMKNLVLKLRASSHNLQNYISSRRKSPAYDGNTSRKPPNEFLTSVVELIGAAKALLAWLDR
  APFTGITDLSVTKNKIIQLCLDLTTAVQKDCLVAEMEDKVLNVVKVLNGICDKMMHSTTDPVMSQCACLE
  EVHLPNVRPGEGLGMYIKSTYDGLHVITGTTENSPADRSQKIHAGDEVIQVNRQTVVGWQLKNLVKKLRE
  NPTGVVLLLKKRPTGSFSFTPAPLKNLRWKPPLVQTSPPPTTTQSPESTMDASLKKEKPAILDLYIPPPP
  AVPYSPRDENVSFSYRGHTKSKQPLPGRKGSESPNSFLDQESQRRRFTITDSDQLPGYSVGTNILPIKMR
  GKTPSYGKPRPLSMPADGNWMGIVDPFAKPRGHGRKGEDALCRYFSNERITPIIEESASPVYRFSRPLTE
  RHLVRGADYIRGSRCYINSDLHSSATIPFQEEGPKKKSASSSAKASSGEPSLLVSWLTRLKLLTH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012061
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012061.1, NP_001012061.1
• RefSeq Size: 3350 bp
• RefSeq ORF: 1668 bp
• Locus ID: 308113
• UniProt ID: Q5SGD7
• Cytogenetics: 1q11
• MW: 61.9 kDa
• Gene Summary: Involved in transepithelial sodium transport. Regulates aldosterone-induced and epithelial sodium channel (ENaC)-mediated sodium transport through regulation of ENaC cell surface expression. Acts as a scaffold protein coordinating the assembly of an ENaC-regulatory complex (ERC).[UniProtKB/Swiss-Prot Function]