Ppp3cc (NM_134367) Rat Tagged ORF Clone

CAT#: RR208106

Ppp3cc (Myc-DDK-tagged ORF) - Rat protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc), (10 ug)

Product Images

Circular map for RR208106

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp3cc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208106 representing NM_134367
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGGAGGCGCCCTCAGTTCTCCACGACCGAGCGCGTCATCAAAGCTGTCCCCTTTCCTCCAACCC
  GACGGCTAACTTTGAAGGAAGTATTTGAGAATGGGAAACCTAAAATGGATGTTTTGAAAAACCATTTGGT
  AAAAGAAGGCCGGGTAGAAGAGGACGTAGCCTTAAAGATAATCAATGATGGGGCTGCCATCCTGAAGCAA
  GAGAAGACTATGCTAGAAGTGGAAGCTCCAATCACAGTGTGTGGTGATGTTCATGGACAATTCTTTGACC
  TGATGAAGTTGTTTGAAGTTGGGGGATCACCTAGTAATACTCGCTACCTCTTCCTGGGTGACTATGTGGA
  CAGAGGCTATTTCAGTATAGAGTGTGTGCTGTATCTGTGGAGCTTAAAGATTAACCATCCTAAAACATTG
  TTTCTGCTTCGAGGGAATCATGAATGCAGGCATCTTACAGAGTACTTCACCTTCAAACAAGAATGTCGGA
  TCAAGTATTCAGAGCTGGTGTACGAAGCGTGCATGCACACTTTCGACTGTCTTCCTCTTGCTGCCCTCTT
  AAACCAGCAGTTTCTCTGTGTACACGGAGGAATGTCTCCTGAAATTACTTGTTTAGACGACATTAGGAAA
  TTAGATAGGTTTGCTGAACCTCCTGCATTTGGGCCAGTGTGCGATCTGCTTTGGTCTGATCCCTTAGAGG
  ATTATGGTAGTGAGAAGACCCTGGAGCACTACACCCACAACACTGTCCGAGGCTGCTCCTACTTCTTCAG
  TTACCCTGCAGTTTGTGAATTTTTACAGAACAATAGTTTATTATCAATAATCAGAGCCCATGAAGCCCAG
  GATGCTGGGTACCGAATGTACAGGAAGAACCAAGCAACTGGCTTTCCATCACTCATTACAATTTTCTCTG
  CCCCCAATTACCTAGATGTCTATAACAATAAAGCTGCAGTATTGAAATATGAAAACAATGTCATGAACAT
  CAGGCAGTTCAACTGTTCTCCACACCCTTACTGGCTCCCAAACTTCATGGATGTTTTCACATGGTCTTTG
  CCTTTTGTTGGAGAAAAAGTCACAGAGATGTTGGTCAATATTCTCAACATATGCTCGGATGAAGAAATGT
  CCTTAGTCGAAGAGGAAGGAGCCACTTCAGTTCGAAAAGAAGTCATCAGGAATAAAATCCGAGCCATTGG
  GAAAATGGCACGGGTCTTTACAGTTCTTCGGGAAGAGAGTGAGAATGTGCTGATTCTCAAGGGCCTCACT
  CCCACAGGCACACTCCCACTCGGGGTCCTCTCTGGAGGAAAGCAGACCATTGAGACTGCCAAACTACAAG
  CAGCAGAGGAGCGGGAAGCCATCAGAGGTTTTACAATTGCACACAGGATCCGAAGTTTTGAAGAAGCCCG
  AGGTCTAGACCGAATTAATGAGAGAATGCCACCCCGAAAAGAGGCTTCTTATCACCATGATGCAGGAAAG
  ACACACCCACACCCGTCAAAAAGGAGCGACCATGGAAAGAAAGCCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208106 representing NM_134367
  Red=Cloning site Green=Tags(s)
  
  MSGRRPQFSTTERVIKAVPFPPTRRLTLKEVFENGKPKMDVLKNHLVKEGRVEEDVALKIINDGAAILKQ
  EKTMLEVEAPITVCGDVHGQFFDLMKLFEVGGSPSNTRYLFLGDYVDRGYFSIECVLYLWSLKINHPKTL
  FLLRGNHECRHLTEYFTFKQECRIKYSELVYEACMHTFDCLPLAALLNQQFLCVHGGMSPEITCLDDIRK
  LDRFAEPPAFGPVCDLLWSDPLEDYGSEKTLEHYTHNTVRGCSYFFSYPAVCEFLQNNSLLSIIRAHEAQ
  DAGYRMYRKNQATGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSL
  PFVGEKVTEMLVNILNICSDEEMSLVEEEGATSVRKEVIRNKIRAIGKMARVFTVLREESENVLILKGLT
  PTGTLPLGVLSGGKQTIETAKLQAAEEREAIRGFTIAHRIRSFEEARGLDRINERMPPRKEASYHHDAGK
  THPHPSKRSDHGKKAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134367
• ORF Size: 1518 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134367.1, NP_599194.1
• RefSeq Size: 1842 bp
• RefSeq ORF: 1521 bp
• Locus ID: 171378
• Cytogenetics: 15p11
• MW: 57.8 kDa
• Gene Summary: involved in skeletal muscle hypertrophy and fiber type conversions [RGD, Feb 2006]