Txnip (NM_001008767) Rat Tagged ORF Clone

CAT#: RR208024

Txnip (Myc-DDK-tagged ORF) - Rat thioredoxin interacting protein (Txnip), (10 ug)

Product Images

Circular map for RR208024

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Txnip
• Synonyms: Vdup1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208024 representing NM_001008767
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGATGTTCAAGAAGATCAAGTCTTTTGAGGTGGTCTTCAACGACCCTGAGAAGGTGTATGGCAGCG
  GGGAGAAGGTGGCCGGCCGGGTGACAGTGGAAGTGTGTGAAGTTACCCGAGTCAAAGCCGTCAGGATCCT
  GGCTTGCGGTGTGGCCAAGGTCCTGTGGATGCAAGGGTCTCAGCAGTGCAAACAGACCTTGGACTACTTG
  CGCTATGAAGACACCCTTCTCCTAGAAGACCAGCCTACAGGTGAGAACGAGATGGTGATCATGAGACCAG
  GAAACAAATATGAGTACAAGTTCGGCTTTGAGCTTCCTCAAGGGCCCCTGGGAACATCCTTCAAAGGGAA
  ATATGGCTGTGTAGACTACTGGGTGAAGGCTTTTCTCGATCGTCCCAGCCAGCCAACTCAAGAGGCAAAG
  AAAAACTTTGAAGTGATGGATCTAGTGGACGTCAATACTCCTGACTTAATGGCACCAGTGTCTGCCAAAA
  AGGAGAAGAAAGTTTCCTGCATGTTCATTCCTGATGGACGCGTGTCAGTCTCCGCTCGAATCGACAGAAA
  AGGATTCTGTGAAGGTGATGACATTTCCATCCACGCTGACTTTGAGAACACATGTTCCCGAATCGTGGTC
  CCCAAAGCAGCTATTGTGGCCCGGCACACTTACCTTGCCAATGGCCAGACCAAAGTGCTCACTCAGAAGC
  TGTCCTCAGTCAGAGGCAATCACATTATCTCAGGGACTTGCGCATCATGGCGTGGCAAGAGTCTCCGAGT
  GCAGAAGATCAGACCGTCCATTCTGGGCTGCAACATCCTCAGAGTTGAATACTCCTTGCTGATCTATGTT
  AGTGTCCCTGGCTCCAAGAAAGTCATCCTCGATCTGCCCCTAGTGATTGGCAGCAGGTCGGGCCTAAGCA
  GCCGGACATCCAGCATGGCTAGCCGGACAAGCTCTGAAATGAGCTGGATAGACCTAAACATCCCAGACAC
  CCCAGAAGCTCCACCTTGCTATATGGACGTGATTCCTGAAGATCACAGACTGGAGAGCCCCACCACCCCC
  CTGCTAGATGATGTGGATGATTCTCAAGACAGCCCTATCTTTATGTACGCCCCCGAGTTCCAGTTCATGC
  CTCCGCCCACTTACACTGAGGTGGATCCCTGCGTCCTTAACAACAACAACAACAACGTGCAGCTCCGCCC
  ACTTACACTGAGGTGGATCCCTGCGTCCTTAACAACAACAACAACAACGTGCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208024 representing NM_001008767
  Red=Cloning site Green=Tags(s)
  
  MVMFKKIKSFEVVFNDPEKVYGSGEKVAGRVTVEVCEVTRVKAVRILACGVAKVLWMQGSQQCKQTLDYL
  RYEDTLLLEDQPTGENEMVIMRPGNKYEYKFGFELPQGPLGTSFKGKYGCVDYWVKAFLDRPSQPTQEAK
  KNFEVMDLVDVNTPDLMAPVSAKKEKKVSCMFIPDGRVSVSARIDRKGFCEGDDISIHADFENTCSRIVV
  PKAAIVARHTYLANGQTKVLTQKLSSVRGNHIISGTCASWRGKSLRVQKIRPSILGCNILRVEYSLLIYV
  SVPGSKKVILDLPLVIGSRSGLSSRTSSMASRTSSEMSWIDLNIPDTPEAPPCYMDVIPEDHRLESPTTP
  LLDDVDDSQDSPIFMYAPEFQFMPPPTYTEVDPCVLNNNNNNVQLRPLTLRWIPASLTTTTTTC
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008767
• ORF Size: 1182 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2771 bp
• RefSeq ORF: 1185 bp
• Locus ID: 117514
• UniProt ID: Q5M7W1
• Cytogenetics: 2q34
• MW: 44 kDa
• Gene Summary: regulates thioredoxin to play an important role in the preservation of cellular viability [RGD, Feb 2006]