Igfals (NM_053329) Rat Tagged ORF Clone

CAT#: RR207864

Igfals (Myc-DDK-tagged ORF) - Rat insulin-like growth factor binding protein, acid labile subunit (Igfals), (10 ug)

Product Images

Circular map for RR207864

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Igfals
• Synonyms: Als
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207864 representing NM_053329
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGCCCTGAGGACAGGAGGCCCAGCCCTGGTGGTGCTTCTGGCTTTCTGGGTGGCACTGGGCCCCTGTC
  ACCTGCAGGGGACAGATCCCGGAGCGTCGGCAGATGCCGAGGGCCCCCAGTGCCCCGTCGCCTGTACCTG
  CAGCCATGATGACTACACAGATGAGCTCAGCGTCTTTTGCAGTTCAAAGAACCTCACACATCTGCCTGAT
  GACATCCCAGTCAGCACCAGAGCCCTGTGGCTTGATGGCAACAACCTCTCTTCTATCCCCTCAGCGGCCT
  TCCAGAACCTGTCCAGCCTGGACTTTCTCAACCTGCAGGGCAGCTGGCTGAGGAGCCTGGAGCCACAGGC
  ACTGCTGGGGCTGCAGAACCTCTACTATCTGCACCTGGAACGGAACCGGCTCCGGAACCTCGCCGTGGGC
  TTGTTCACACACACACCGAGTCTGGCTTCACTCAGCCTGAGCAGCAACCTCTTGGGCCGGCTGGAGGAAG
  GGCTGTTCCAGGGCCTCAGTCACCTTTGGGACCTCAACCTGGGTTGGAACAGTCTAGTGGTCCTGCCTGA
  CACAGTGTTCCAGGGACTGGGCAACCTCCACGAGCTGGTGCTGGCTGGCAACAAACTGACTTACCTGCAG
  CCTGCGCTCTTCTGTGGCTTGGGCGAGCTGCGGGAGCTGGATCTGAGCAGGAACGCACTCCGAAGCGTCA
  AAGCTAACGTCTTTGTACATTTGCCCAGGCTGCAGAAGCTGTACCTGGACCGGAACCTCATTACAGCCGT
  GGCCCCTGGTGCCTTTCTGGGCATGAAGGCCCTGCGTTGGCTGGACCTGTCGCACAACCGCGTGGCTGGC
  CTCATGGAGGACACCTTCCCAGGCCTGCTGGGCCTGCACGTCCTGCGCCTGGCACACAATGCGATCGCTA
  GCTTGCGGCCGCGCACTTTCAAAGACCTGCACTTCCTGGAGGAACTGCAGCTGGGCCACAATCGAATCAG
  GCAGCTCGGGGAGAGGACATTCGAGGGCCTGGGGCAGCTGGAGGTGCTGACGCTCAATGACAACCAGATC
  ACTGAGGTCAGGGTGGGCGCCTTCTCTGGCCTTTTCAATGTGGCGGTTATGAATCTCTCCGGCAACTGTC
  TGAGGAGCCTCCCGGAGCGGGTGTTTCAGGGTCTGGACAAACTGCACAGCCTGCACCTAGAGCACAGCTG
  CCTGGGTCACGTCCGCCTGCACACTTTTGCTGGCCTCTCAGGGCTGCGCAGGCTCTTCCTCAGGGACAAC
  AGCATCTCCAGCATCGAAGAACAGAGCCTGGCAGGGCTTTCGGAGCTCCTGGAACTGGATCTTACTACCA
  ACCGCCTCACACATCTGCCCCGCCAGCTCTTCCAGGGCCTCGGCCACCTGGAGTACCTGCTTCTCTCCTA
  CAACCAACTGACGACTTTATCCGCGGAGGTCCTGGGCCCTCTGCAGCGGGCCTTCTGGTTGGATATCTCA
  CACAACCACCTGGAGACGCTGGCCGAAGGCCTTTTCTCATCTCTGGGGCGCGTTCGCTACCTCAGCCTCA
  GGAATAACTCCTTGCAGACCTTTTCACCACAGCCCGGCCTGGAGCGCCTGTGGCTTGATGCCAACCCCTG
  GGACTGCAGCTGTCCCCTCAAGGCGCTTCGAGACTTTGCCCTGCAGAACCCTGGTGTTGTCCCCCGCTTT
  GTTCAGACTGTCTGTGAGGGGGACGACTGCCAGCCGGTGTACACCTACAACAATATCACTTGCGCTGGCC
  CCGCCAACGTCTCGGGCCTCGACCTAAGAGACGTTAGTGAAACACATTTTGTGCACTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207864 representing NM_053329
  Red=Cloning site Green=Tags(s)
  
  MALRTGGPALVVLLAFWVALGPCHLQGTDPGASADAEGPQCPVACTCSHDDYTDELSVFCSSKNLTHLPD
  DIPVSTRALWLDGNNLSSIPSAAFQNLSSLDFLNLQGSWLRSLEPQALLGLQNLYYLHLERNRLRNLAVG
  LFTHTPSLASLSLSSNLLGRLEEGLFQGLSHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKLTYLQ
  PALFCGLGELRELDLSRNALRSVKANVFVHLPRLQKLYLDRNLITAVAPGAFLGMKALRWLDLSHNRVAG
  LMEDTFPGLLGLHVLRLAHNAIASLRPRTFKDLHFLEELQLGHNRIRQLGERTFEGLGQLEVLTLNDNQI
  TEVRVGAFSGLFNVAVMNLSGNCLRSLPERVFQGLDKLHSLHLEHSCLGHVRLHTFAGLSGLRRLFLRDN
  SISSIEEQSLAGLSELLELDLTTNRLTHLPRQLFQGLGHLEYLLLSYNQLTTLSAEVLGPLQRAFWLDIS
  HNHLETLAEGLFSSLGRVRYLSLRNNSLQTFSPQPGLERLWLDANPWDCSCPLKALRDFALQNPGVVPRF
  VQTVCEGDDCQPVYTYNNITCAGPANVSGLDLRDVSETHFVHC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053329
• ORF Size: 1809 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053329.2, NP_445781.2
• RefSeq Size: 2190 bp
• RefSeq ORF: 1812 bp
• Locus ID: 79438
• UniProt ID: P35859
• Cytogenetics: 10q12
• MW: 66.8 kDa
• Gene Summary: subunit of the ternary insulin-like growth factor-binding protein complex; involved in controlling the bioavailability of circulating insulin-like growth factors [RGD, Feb 2006]