Anapc5 (NM_001080147) Rat Tagged ORF Clone

CAT#: RR207668

Anapc5 (Myc-DDK-tagged ORF) - Rat anaphase-promoting complex subunit 5 (Anapc5), (10 ug)

Product Images

Circular map for RR207668

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Anapc5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207668 representing NM_001080147
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGACCAACGGGGTAGTGCACGCCAACTTGTTTGGCATCAAGGACTGGGTGACGCCCTATAAGATCG
  CGGTCCTGGTGCTGCTGAACGAGATGGGCCGCACGGGAGAGGGTGCTGTCAGCCTTGTGGAGCGGCGGAA
  GCTCAACCAGCTGCTCCTGCCCCTGCTGCAGGGCCCAGATATTACACTGTCAAAGCTGTACAAACTAATT
  GAAGAATCGTGTCCTCAGCTGGCAAATTCAGTGCAGATCAGAATCAAACTGATGGCTGAAGGCGAATTGA
  AGGATCTGGAACAATTTTTTGATGACCTTTCAGATTCTTTTTCTGGAACTGAACCAGAGGTTCACAAAAC
  AAGTGTAGTAGGTCTGTTTCTGCGTCACATGATCTTGGCCTACAGCAAGCTTTCCTTCAGTCAAGTGTTC
  AAGCTGTACACTGCCCTGCAGCAGTATTTCCAGAACGGGGAGAAAAAGACGGTGGAAGACGCTGACATGG
  ACAGAGAGGATGGAGAGAGACAGATGGAGAAGGAGGAGCTCGACGTATCTGTCAGAGAAGAGGAGGTATC
  TTGCAGTGGTCCTCTGTCCCAAAAACAAGCAGAATTTTTTCTTTCTCAGCAGGCTGCTTTGTTGAAGAAT
  GACGAGACTAAAGCCCTCACCCCAGCCTCCTTGCAGAAGGAGTTGAACAACCTGTTGAAATTTAATCCTG
  ATTTTGCTGAAGCTCATTACCTCAGCTACTTAAACAACCTCCGTGTTCAAGATGTTTTCAGCTCAACGCA
  CAGCCTCCTTCATTATTTTGACCGCCTGATTCTCACTGGAGCAGAGGGCAAAAGTAATGGGGAAGAAGGT
  TATGGCCGGAGCCTAAGATACGCCGCTCTCAACCTGGCTGCCCTGCACTGCCGCTTCGGCCACTATCAAC
  AGGCAGAGCTCGCCCTGCAGGAGGCAATTAGGATTGCCCAGGAGTCCAACGATCACGTGTGTCTGCAGCA
  CTGTTTGAGCTGGCTTTATGTGCTGGGGCAGAAGAGAGCCGATAGCTATGTTCTGCTGGAGCATTCTGTG
  AAGAAAGCAGTGCATTTTGGGTTACCGAGAGCTTTTGCTGGGAAGACGGCCAACAAACTGATGGATGCCC
  TAAAGGACTCTGACCTCCTGCACTGGAAACACAGCTTGTCAGAACTTATCGACATCAGCATCGCACAGAA
  AACGGCCATCTGGAGGCTGTACGGCCGCAGCACCATGGCACTGCAGCAAGCCCAGATGCTGCTGAGCATG
  AACAGCCTGGAGTCACTGAGTGCAGGTGTGCAACAGAACAACACCGAGTCCTTTGCCGTCGCTCTCTGCC
  ATCTTGCAGAGCTGCATGCAGAACAGGGCTGTTTCGCTGCTGCCGGGGAAGTACTGAAGCACTTGAAGGA
  ACGGTTCCCACCCAACAGTCAGCACGCCCAGTTATGGATGCTGTGTGATCAAAAAATACAGTTTGACAGA
  GCAATGAATGATGGTAAATTCCATTTGGCTGACTCGCTTGTTACAGGAATCACAGCACTTAATGGCATAG
  AAGGAGTATACAGAAAAGCAGTGGTGCTGCAAGCTCAAAACCAGATGACAGAGGCACACAAGCTTTTGCA
  GAAGTTGCTGACATACTGTCAGAAGTTGAAGAACACAGAGATGGTCATCAGCGTCCTCCTGTCGGTGGCA
  GAGCTGTACTGGCGATCTTCCTCCCCGACCATCGCAATGCCTGTGCTCCTGGAAGCTCTGGCCCTCTCCA
  AAGAATACCGGTTACAGTACTTGGCCTCTGAAACTGTGCTCAACTTGGCTTATGCCCAGCTCATCCTGGG
  GATCCCAGAACAGGCCTTAACCCTTCTCCACATGGCTATTGAGCCCATCCTGGCCGATGGGGCCATCCTG
  GACAAAGGCCGTGCCATGTTCTTAGTGTCCAAGTGCCAAGTGGCTTCAGCAGCTTCCTATGACCCAGTGA
  AGAAAGCAGAAGCTCTGGAAGCAGCCATTCAGAACCTCACTGAAGCCAAGAACTACTTTGCAAAAGTCGA
  CTGTAGAGAGCGCATCAGGGATGTGTCGTACTTCCAGGCCAGGCTGTACCACGCCCTCGGCAAGACCCAG
  GAGAGGAACCACTGTGCCATGGTCTTCCGGCAGCTGCACCAGGAGCTGCCTTCCCATGGGGTGCCCCTGA
  TTAACCATCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207668 representing NM_001080147
  Red=Cloning site Green=Tags(s)
  
  MMTNGVVHANLFGIKDWVTPYKIAVLVLLNEMGRTGEGAVSLVERRKLNQLLLPLLQGPDITLSKLYKLI
  EESCPQLANSVQIRIKLMAEGELKDLEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVF
  KLYTALQQYFQNGEKKTVEDADMDREDGERQMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQAALLKN
  DETKALTPASLQKELNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAEGKSNGEEG
  YGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCLSWLYVLGQKRADSYVLLEHSV
  KKAVHFGLPRAFAGKTANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLSM
  NSLESLSAGVQQNNTESFAVALCHLAELHAEQGCFAAAGEVLKHLKERFPPNSQHAQLWMLCDQKIQFDR
  AMNDGKFHLADSLVTGITALNGIEGVYRKAVVLQAQNQMTEAHKLLQKLLTYCQKLKNTEMVISVLLSVA
  ELYWRSSSPTIAMPVLLEALALSKEYRLQYLASETVLNLAYAQLILGIPEQALTLLHMAIEPILADGAIL
  DKGRAMFLVSKCQVASAASYDPVKKAEALEAAIQNLTEAKNYFAKVDCRERIRDVSYFQARLYHALGKTQ
  ERNHCAMVFRQLHQELPSHGVPLINHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080147
• ORF Size: 2181 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080147.1, NP_001073616.1
• RefSeq Size: 2384 bp
• RefSeq ORF: 2184 bp
• Locus ID: 288671
• UniProt ID: A1L1K3
• Cytogenetics: 12q16
• MW: 81.7 kDa
• Gene Summary: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]