Bmp3 (NM_017105) Rat Tagged ORF Clone

CAT#: RR207491

Bmp3 (Myc-DDK-tagged ORF) - Rat bone morphogenetic protein 3 (Bmp3), (10 ug)

Product Images

Circular map for RR207491

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bmp3
• Synonyms: PBMP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207491 representing NM_017105
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGGCTCGCGGGCTGCTGTGTCTGTGGCTAGGTTGTTTCTGCCTGAACCTGGCACAGGGACAGA
  GACCAAATTTGCACCTCCCGGGACTCCGCGGGACTGAGTCAAGCGACCGCATGACAGGTGGTGGCCCGAG
  CCCGGACCTGAGGCCTCACGACAAGGTGTCAGAGCATATGCTGTGGCTCTATGACAGGTACAGCGGCAGC
  AACAGAGCCCAGGCTACCCGGACACCGGGCTCGCAGCTCCCGGGTCCCCAACCCCTGCGTGGCGGTAACA
  CGGTTCGTAGCTTCAGAGCCGCAGCCGCAGGAACTCTTCAAAGAAAGGGCTTGCACACTTTTAATCTGAC
  TTCTCTAACCAAGTCTGAGAACATTTTGTCAGCCACACTGTATTTCTACATTGGAGAATTGGTGAACACC
  AGTGTGAACTGTCCAGAATCCCAAGGTTGTTCCCATGACAGCCAGAGACAACACATCCAGATAGACCTCT
  CTGCATGGACCCTCCAATCCAACCAAAGCCAGCTCTTGGGTCATCTGTCAGTAGATACGGCCAAACCTTA
  TAGAGACAGCATGTCTTGGCTGTCAAAAGACATCACTCAGCTCTTACGGAAGGCCAAGCAAGACGAGGAG
  TTTCTCATAGGATTTAACATTACCTCCAGAGCACACGAGCTTCCCAAGAGGATGCTGCTTTTCCCGGAAC
  CTTATATCTTGGTGTATGCCAACGATGCCGCCATTTGTGAGCCTGAAAGCGTGGTATCTAGCCTACAGAG
  ACACCGCGATTTCACAGCAGGAACTGTGCCCAGACTGGATAGCCACGTCAGAGAAGCCCTCTCTGTTGAG
  AGGAGGAAGAAGCGCTCTACTGGGATCTTGTTGCCATTGCAGAACAACGAGCTCCCTGGGGCCGAGTATC
  AGTACAAGGAGGCTGGAGTGTGGGAGGAGAGAAAGCCTTACAAGAGCCTTCAGACTCAGCCTCCTGAAAA
  GAGCCGGAGCAAAAAGAAACAGAGGAAAGGGCCTCATCAAAAGGGACAGACACTGCAGTTTGATGAGCAG
  ACACTGAAGAAGGCGAGGCGAAAGCAGTGGATCGAGCCTCGGAACTGTGCCCGCAGGTACCTTAAAGTGG
  ACTTTGCCGATATTGGCTGGAGCGAATGGATTATCTCCCCCAAGTCATTTGATGCCTACTATTGCTCCGG
  AGCCTGCCAGTTCCCCATGCCAAAGTCTTTGAAGCCATCAAATCACGCCACCATCCAGAGCATAGTGAGA
  GCCGTGGGGGTCGTCTCTGGGATTCCTGAGCCCTGCTGTGTTCCGGAGAAGATGTCCTCGCTCAGCATCT
  TGTTCTTTGACGAGAACAAGAATGTGGTCCTCAAAGTCTATCCTAACATGACAGTAGACTCTTGTGCTTG
  TAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207491 representing NM_017105
  Red=Cloning site Green=Tags(s)
  
  MAGARGLLCLWLGCFCLNLAQGQRPNLHLPGLRGTESSDRMTGGGPSPDLRPHDKVSEHMLWLYDRYSGS
  NRAQATRTPGSQLPGPQPLRGGNTVRSFRAAAAGTLQRKGLHTFNLTSLTKSENILSATLYFYIGELVNT
  SVNCPESQGCSHDSQRQHIQIDLSAWTLQSNQSQLLGHLSVDTAKPYRDSMSWLSKDITQLLRKAKQDEE
  FLIGFNITSRAHELPKRMLLFPEPYILVYANDAAICEPESVVSSLQRHRDFTAGTVPRLDSHVREALSVE
  RRKKRSTGILLPLQNNELPGAEYQYKEAGVWEERKPYKSLQTQPPEKSRSKKKQRKGPHQKGQTLQFDEQ
  TLKKARRKQWIEPRNCARRYLKVDFADIGWSEWIISPKSFDAYYCSGACQFPMPKSLKPSNHATIQSIVR
  AVGVVSGIPEPCCVPEKMSSLSILFFDENKNVVLKVYPNMTVDSCACR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017105
• ORF Size: 1404 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017105.1, NP_058801.1
• RefSeq Size: 2158 bp
• RefSeq ORF: 1407 bp
• Locus ID: 25667
• UniProt ID: P49002
• Cytogenetics: 14p22
• MW: 52.7 kDa
• Gene Summary: may play a role in bone and cartilage formation [RGD, Feb 2006]