Ppp3r1 (NM_017309) Rat Tagged ORF Clone

CAT#: RR207229

Ppp3r1 (Myc-DDK-tagged ORF) - Rat protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) (Ppp3r1), (10 ug)

Product Images

Circular map for RR207229

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp3r1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207229 representing NM_017309
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAATGAGGCGAGTTACCCTTTGGAAATGTGCTCACACTTCGATGCTGATGAGATTAAAAGGCTAG
  GAAAGAGATTCAAGAAGCTTGACTTGGACAACTCTGGTTCTTTGAGCGTGGAGGAGTTCATGTCTCTGCC
  TGAGTTACAACAGAACCCTTTAGTACAGCGGGTCATAGATATATTCGACACAGACGGGAATGGAGAAGTG
  GACTTCAAAGAATTCATTGAAGGAGTCTCTCAGTTCAGTGTCAAAGGCGATAAGGAACAGAAGTTGAGGT
  TCGCTTTCCGTATCTACGACATGGATAAAGACGGCTATATTTCCAATGGAGAGCTCTTCCAGGTGTTGAA
  GATGATGGTGGGCAACAACCTGAAAGATACGCAGTTACAGCAGATTGTAGACAAAACCATAATAAACGCA
  GATAAGGACGGGGACGGGAGAATATCCTTTGAGGAGTTCTGTGCTGTTGTAGGTGGCCTAGATATCCACA
  AAAAGATGGTGGTGGATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207229 representing NM_017309
  Red=Cloning site Green=Tags(s)
  
  MGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEV
  DFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINA
  DKDGDGRISFEEFCAVVGGLDIHKKMVVDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017309
• ORF Size: 510 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017309.2, NP_059005.1
• RefSeq Size: 2803 bp
• RefSeq ORF: 513 bp
• Locus ID: 29748
• UniProt ID: P63100
• Cytogenetics: 14q22
• MW: 19.3 kDa
• Gene Summary: regulatory subunit of the enzyme that catalyzes the calcium-dependent removal of serine- and threonine-bound phosphate groups [RGD, Feb 2006]