Dis3 (NM_001127483) Rat Tagged ORF Clone

CAT#: RR206696

Dis3 (Myc-DDK-tagged ORF) - Rat DIS3 mitotic control homolog (S. cerevisiae) (Dis3), (10 ug)

Product Images

Circular map for RR206696

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dis3
• Synonyms: RGD1304646
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206696 representing NM_001127483
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAAGTCCAAAACATTCTTAAAGAAGACCCGCGCGGGCGGCGTGGTGAAGATCGTGCGCGAGCACT
  ACCTGCGGGATGACATCGGCTGCGGCACGCCGGCTTGCTCGGCCTGTGGCGCGGCGCACGCGGGCCCGGC
  CCTGGAGCTGCAGCCCCGGGACCAGGCGAGCAGCCTCTGCCCATGGCCGCACTACCTCCTGCCGGACACC
  AATGTGCTGCTGCACCAGATTGATGTTCTTGAGGACCCTGCTATCAGGAACGTAATTGTGCTTCAGACAG
  TCCTGCAGGAAGTGAGAAACCGGAGTGCCCCCATATATAAGCGAATCAGGGATGTGACGAATAACCAGGA
  GAAGCATTTCTATACGTTCACTAACGAGCACCATAAAGAAACCTACATAGAGCAAGAACAGGGGGAGAAT
  GCCAATGACAGAAATGACAGAGCCATTCGAGTAGCAGCAAAGTGGTACAATGAACATCTAAAGATGTCAG
  CAGAAAATCAGCTACAAGTTATCCTTATAACAAACGACAAGAAGAACAAAGAAAAGGCTGCAGAAGAAGG
  AATACCAGCTTTCACATGTGAAGAATATGTAAAGAGCCTGACTGCTAACCCTGAACTTATAGATCGTCTT
  GCTTGTTTGTCTGATGAGATGAATGAAATAGAAAGTGGGAAAATAATATTTTCAGAGCATCTTCCTTTAA
  GCAAGCTCCAACAAGGCATAAAATCTGGTTCATACCTTCAAGGAACATTCAGAGCTAGCAGGGAAAATTA
  CTTAGAAGCTACAGTGTGGATTCATGGAGACAAAGAAGATGAAAAAGAGATACTTATACAAGGACTTAAA
  CATCTAAACAGAGCTATTCATGAAGATATTGTGGCTGTGGAACTATTGCCCAAGAGTCAGTGGGTGGCAC
  CATCTTCTGTGGTTTTACATGATGAAGGTCAAAATGAAGATGATGTGGAGAAAGATGAGGAGAGAGAACT
  CCTGCTTAAGACTGCTGTAAGTGAAAAAATGTTGAGGCCTACAGGTAGAGTTGTGGGGATAATAAAGAGG
  AATTGGAGACCGTACTGTGGCATGCTTTCCAAGTCTGATATTAAGGAGTCAAGAAGACATCTCTTTACAC
  CTGCTGATAAGAGAATTCCACGAATTCGGATAGAAACCAGACAGGCTTCTGCATTAGAAGGACGGAGAAT
  TATTGTCGCTATTGATGGTTGGCCCAGAAATTCTAGATATCCAAATGGACACTTTGTAAAAAATTTAGGT
  GATGTTGGAGAGAAAGAGACGGAAACGGAAGTTTTGCTGCTTGAGCATGACGTTCCTCATCAGCCCTTTT
  CCCAGGCTGTTCTTAGCTTCCTGCCGAAGATGCCCTGGAGCATTACTGAGGAGGACATGAAAAACCGAGA
  AGATCTGAGACATCTGTGTGTTTGCAGTGTGGATCCTCCAGGATGTACTGACATAGATGATGCCCTGCAC
  TGCAGAGAACTCAGCAATGGAAACTTGGAGGTTGGTGTTCATATTGCGGATGTTAGCCATTTTATCAGGC
  CAGGAAATGCATTGGATCAAGAATCAGCAAGAAGAGGAACAACTGTTTATCTTTGTGAAAAGAGGATTGA
  CATGGTCCCAGAGCTGCTCAGCTCCAACCTCTGTTCCTTAAGATCCAACGTGGACAGGTTGGCATTTTCC
  TGTATTTGGGAAATGAATCATAATGCTGAAATATTAAAAACGAGATTTACCAAAAGTGTCATTAATTCAA
  AGGCTTCTCTTACATATGCTGAAGCACAGATGAGAATTGATTCAGCCGCTATGAATGATGACATTACCAC
  TAGTCTCCGTGGACTGAATAAACTAGCTAAAATTCTAAAAAGGGGAAGGATTGAAAAGGGGGCTTTGACA
  CTCTCTTCTCCAGAAATTCGATTTCACATGGACAGTGAAACTCACGACCCAATTGACCTGCAGACCAAGG
  AGCTGAGAGAAACAAATTCCATGGTGGAAGAATTTATGTTACTCGCTAATATTTCTGTTGCAAAAAAAAT
  TCATGAAGAGTTTTCTGAACATGCTTTGCTTCGAAAACATCCAGCCCCACCTCCCTCCAATTATGAAGTT
  CTTGTTAAGGCCGCTAAGTCCAAGAATTTGGAAATTCGGACTGATACAGCCAAATCTTTGGCTGACTCTT
  TGGACCGGGCTGAATGTCCTGATTTCCCATACCTGAATACTCTGTTAAGGATATTGGCCACTCGCTGTAT
  GATGCAAGCTGTCTACTTCTGTTCTGGGATGGATAGTGACTTTCATCACTATGGCTTAGCCTCCCCAATA
  TACACACATTTCACTTCTCCTATCAGAAGATATGCAGACATAATTGTTCATCGGCTATTAGCTGTGGCGA
  TTGGGGCTGATTGTACTTATCCAGAGTTGACGGACAAACACAAGCTTTCAGATATATGTAAAAACCTCAA
  TTTCCGGCATAAAATGGCTCAGTATGCCCAGCGTGCGTCAGTAGCTTTTCATACACAGTTGTTTTTCAAA
  AGCAAAGGAATAGTAAGCGAAGAAGCCTATATTCTCTTTGTAAGAAAAAATGCAATTGTAGTGTTAATTC
  CAAAGTATGGCTTAGAAGGTACAGTTTTTTTTGAAGAAAAAGATAAACCAAAGCCACGCCTTACTTATGA
  TGATGAGATACCTTCACTCAGAATAGAAGGTACAGTGTTCCATGTGTTTGATAAAGTTAAAGTGAAAATC
  ACATTAGATTCATCAAATCTTCAACACCAGAAAATCCGCATGGCCCTTGTAGAACCACAGATACCAGGAA
  TAAATATGCCTCCTGATGTTTCAGACATGGATCTTAATGGACCAGGGGGAAAGAAGAGGAAACTTGAAAA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206696 representing NM_001127483
  Red=Cloning site Green=Tags(s)
  
  MLKSKTFLKKTRAGGVVKIVREHYLRDDIGCGTPACSACGAAHAGPALELQPRDQASSLCPWPHYLLPDT
  NVLLHQIDVLEDPAIRNVIVLQTVLQEVRNRSAPIYKRIRDVTNNQEKHFYTFTNEHHKETYIEQEQGEN
  ANDRNDRAIRVAAKWYNEHLKMSAENQLQVILITNDKKNKEKAAEEGIPAFTCEEYVKSLTANPELIDRL
  ACLSDEMNEIESGKIIFSEHLPLSKLQQGIKSGSYLQGTFRASRENYLEATVWIHGDKEDEKEILIQGLK
  HLNRAIHEDIVAVELLPKSQWVAPSSVVLHDEGQNEDDVEKDEERELLLKTAVSEKMLRPTGRVVGIIKR
  NWRPYCGMLSKSDIKESRRHLFTPADKRIPRIRIETRQASALEGRRIIVAIDGWPRNSRYPNGHFVKNLG
  DVGEKETETEVLLLEHDVPHQPFSQAVLSFLPKMPWSITEEDMKNREDLRHLCVCSVDPPGCTDIDDALH
  CRELSNGNLEVGVHIADVSHFIRPGNALDQESARRGTTVYLCEKRIDMVPELLSSNLCSLRSNVDRLAFS
  CIWEMNHNAEILKTRFTKSVINSKASLTYAEAQMRIDSAAMNDDITTSLRGLNKLAKILKRGRIEKGALT
  LSSPEIRFHMDSETHDPIDLQTKELRETNSMVEEFMLLANISVAKKIHEEFSEHALLRKHPAPPPSNYEV
  LVKAAKSKNLEIRTDTAKSLADSLDRAECPDFPYLNTLLRILATRCMMQAVYFCSGMDSDFHHYGLASPI
  YTHFTSPIRRYADIIVHRLLAVAIGADCTYPELTDKHKLSDICKNLNFRHKMAQYAQRASVAFHTQLFFK
  SKGIVSEEAYILFVRKNAIVVLIPKYGLEGTVFFEEKDKPKPRLTYDDEIPSLRIEGTVFHVFDKVKVKI
  TLDSSNLQHQKIRMALVEPQIPGINMPPDVSDMDLNGPGGKKRKLEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127483
• ORF Size: 2871 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127483.1, NP_001120955.1
• RefSeq Size: 3115 bp
• RefSeq ORF: 2874 bp
• Locus ID: 306103
• Cytogenetics: 15q21
• MW: 108.8 kDa