Spast (NM_001108702) Rat Tagged ORF Clone

CAT#: RR206671

Spast (Myc-DDK-tagged ORF) - Rat spastin (Spast), (10 ug)

Product Images

Circular map for RR206671

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Spast
• Synonyms: Spg4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206671 representing NM_001108702
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCTCCGGCCGGACGACGGAAGAAGAAAGGCTCAGGCGGCGCGAGCCCCGCGCCCGCCAGGCCTC
  CGCCCCCCGCCGCGGTCCCCGCCCCTGCCGCCGGCCCGGCCCCTGCGCCCGGCTCGCCGCATAAGCGGAA
  CCTGTATTACTTCTCGTATCCGCTGGTCGTCGGCTTCGCCCTGCTGCGCCTGCTGGCCTGCCACCTGGGG
  CTCCTCTTCGTGTGGCTCTGCCAGCGCTTCTCCCGCGCCCTCATGGCCGCCAAGAGGAGCTCCGGGACCG
  CGCCGGCGCCCGCCTCGCCCTCGACTCCAGCCCCCGGACCGGGTGGCGAGGCCGAGAGCGTCCGCGTCTT
  CCACAAGCAGGCCTTTGAGTACATCTCCATTGCCCTGCGCATCGACGAGGAAGAGAAAGGACAGAAGGAA
  CAAGCTGTGGAATGGTATAAGAAAGGCATCGAAGAACTAGAGAAAGGAATCGCGGTTATAGTTACGGGCC
  AAGGTGAACAGTACGAAAGAGCTAGACGCCTTCAAGCCAAAATGATGACGAATTTGGTTATGGCTAAGGA
  CCGTTTACAACTCCTAGAAAGTGGAGCAGTTCCGAAGAAGAAAGACCCCTTAACACATGCTAGTAATTCA
  TTGCCTCGATCAAAAACTGTCATGAAAAGTGGATCCACAGGGCTCTCCGGTCACCACAGGGCGCCTAGTT
  GCAGTGGTTTATCCATGGTTTCTGGAGCAAGACCGGGATCTGGTCCTGCAGCTACCACACATAAGGGTAC
  TTCAAAACCAAATAGAACCAACAAACCTTCTACACCCACAACTGCAGTTCGGAAAAAGAAAGACTTGAAA
  AATTTTAGGAATGTGGACAGCAATCTTGCTAACCTTATAATGAATGAAATTGTTGACAATGGGACAGCTG
  TTAAATTTGATGATATAGCTGGTCAGGAACTGGCAAAACAAGCATTGCAAGAGATTGTCATTCTCCCTTC
  TCTGCGGCCTGAGTTGTTCACAGGGCTCAGAGCTCCCGCCAGAGGCTTGTTACTCTTCGGTCCACCAGGA
  AACGGAAAAACCATGCTGGCTAAAGCAGTAGCTGCAGAATCTAATGCGACTTTTTTCAATATAAGTGCTG
  CAAGTTTAACTTCAAAATATGTAGGAGAGGGAGAGAAATTGGTGAGAGCTCTTTTTGCTGTGGCTCGAGA
  ACTTCAACCCTCTATAATTTTTATAGATGAAGTTGATAGTCTTTTGTGTGAGAGAAGAGAAGGGGAGCAC
  GATGCTAGTAGACGACTAAAAACTGAATTTTTAATAGAATTTGATGGTGTGCAGTCTGCTGGAGATGACA
  GAGTCCTCGTAATGGGTGCAACCAACAGGCCCCAAGAGCTGGATGAAGCTGTTCTCAGGCGTTTCATTAA
  ACGGGTGTATGTGTCATTACCCAATGAGGAGACACGACTACTTCTACTTAAAAACCTGTTATGTAAACAA
  GGAAGTCCACTGACCCAAAAAGAACTTGCTCAACTGGCGAGAATGACTGATGGATACTCTGGAAGTGATC
  TGACTGCTTTGGCAAAGGATGCAGCACTGGGTCCTATCCGAGAACTGAAACCAGAGCAGGTGAAGAACAT
  GTCTGCCAGTGAGATGAGAAATATTCGATTATCTGACTTCACAGAATCCTTAAAAAAGATCAAACGCAGT
  GTGAGTCCTCAGACTTTAGAAGCATACATACGCTGGAACAAGGACTTTGGAGACACCACTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206671 representing NM_001108702
  Red=Cloning site Green=Tags(s)
  
  MSSPAGRRKKKGSGGASPAPARPPPPAAVPAPAAGPAPAPGSPHKRNLYYFSYPLVVGFALLRLLACHLG
  LLFVWLCQRFSRALMAAKRSSGTAPAPASPSTPAPGPGGEAESVRVFHKQAFEYISIALRIDEEEKGQKE
  QAVEWYKKGIEELEKGIAVIVTGQGEQYERARRLQAKMMTNLVMAKDRLQLLESGAVPKKKDPLTHASNS
  LPRSKTVMKSGSTGLSGHHRAPSCSGLSMVSGARPGSGPAATTHKGTSKPNRTNKPSTPTTAVRKKKDLK
  NFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPG
  NGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEH
  DASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQ
  GSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRS
  VSPQTLEAYIRWNKDFGDTTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108702
• ORF Size: 1743 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108702.2, NP_001102172.2
• RefSeq Size: 4489 bp
• RefSeq ORF: 1746 bp
• Locus ID: 362700
• UniProt ID: B2RYN7
• Cytogenetics: 6q13
• MW: 63 kDa
• Gene Summary: ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated. Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Severing activity is not dependent on tubulin acetylation or detyrosination. Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex. Recruited at the midbody, probably by IST1, and participates in membrane fission during abscission together with the ESCRT-III complex. Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling. Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing (By similarity). Probably plays a role in axon growth and the formation of axonal branches (PubMed:18234839).[UniProtKB/Swiss-Prot Function]