Hscb (NM_001108340) Rat Tagged ORF Clone

CAT#: RR206250

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  • TrueORF®

Hscb (Myc-DDK-tagged ORF) - Rat HscB iron-sulfur cluster co-chaperone homolog (E. coli) (Hscb), (10 ug)



Reconstitution Protocol

USD 330.00

4 Weeks*

Size
    • 10 ug

Product images

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Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Hscb
Synonyms RGD1311005
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR206250 representing NM_001108340
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGAGCTGCAGGGCTCGGTCCCTGCTGTGCGTGTGGGAGGTGCGACTGGCTAGGTTCCTTGGTAGGA
GCCTGCTGAGCAGCAATGCCGCCTCGGGAAAGAGCATCGAGCCGCAATGCTGGAACTGCGGCCGCGCGAT
GGGAGCCGGGCGTGGGGATGAGTTTTTCTGCGCACACTGCCGCGCGCTGCAGCCTCCTGACCCCACTCGG
GACTACTTCAGCCTCATGAACTGCAACCAATCCTTCAGGGTTGACATCCGGAAACTTCAACAAAGGTACC
AGCAACTACAGCGCCTTGTCCACCCAGATTTCTTCAGCCAAAAGTCTCAGACTGAGAAACGGTTCTCGGA
GAAGCATTCGACCCTGGTGAATGATGCCTACAAGACTCTTCAGGCCCCCGTGAGCAGAGGACTATATCTT
CTAAAGCTCCAAGGAATAGAAATTCCTGAAGGGACAGATTATAGAACAGACAGTCAGTTCCTTGTGGAAA
TCATGGAAATCAATGAAAAACTCGCAGACGCCAAAAGTGAGGCAGCCATGGAAGAGGTAGAAGCCACTGT
CAGAGCTAAACAGAAAGAATTTACGGACAATATAAACAGAGCTTTTGAACAAGGTGATTTTGAAAAAGCC
AAGGAACTTCTTACAAAAATGAGATACTTTTCAAACATAGAAGAAAAGATCAAGTTAAGCAAGAACCCTC
TC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR206250 representing NM_001108340
Red=Cloning site Green=Tags(s)

MWSCRARSLLCVWEVRLARFLGRSLLSSNAASGKSIEPQCWNCGRAMGAGRGDEFFCAHCRALQPPDPTR
DYFSLMNCNQSFRVDIRKLQQRYQQLQRLVHPDFFSQKSQTEKRFSEKHSTLVNDAYKTLQAPVSRGLYL
LKLQGIEIPEGTDYRTDSQFLVEIMEINEKLADAKSEAAMEEVEATVRAKQKEFTDNINRAFEQGDFEKA
KELLTKMRYFSNIEEKIKLSKNPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001108340
ORF Size 702 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001108340.1, NP_001101810.1
RefSeq Size 783 bp
RefSeq ORF 705 bp
Locus ID 360826
Cytogenetics 12q16
MW 27.1 kDa
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.