Hscb (NM_001108340) Rat Tagged ORF Clone

CAT#: RR206250

Hscb (Myc-DDK-tagged ORF) - Rat HscB iron-sulfur cluster co-chaperone homolog (E. coli) (Hscb), (10 ug)

Product Images

Circular map for RR206250

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hscb
• Synonyms: RGD1311005
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206250 representing NM_001108340
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAGCTGCAGGGCTCGGTCCCTGCTGTGCGTGTGGGAGGTGCGACTGGCTAGGTTCCTTGGTAGGA
  GCCTGCTGAGCAGCAATGCCGCCTCGGGAAAGAGCATCGAGCCGCAATGCTGGAACTGCGGCCGCGCGAT
  GGGAGCCGGGCGTGGGGATGAGTTTTTCTGCGCACACTGCCGCGCGCTGCAGCCTCCTGACCCCACTCGG
  GACTACTTCAGCCTCATGAACTGCAACCAATCCTTCAGGGTTGACATCCGGAAACTTCAACAAAGGTACC
  AGCAACTACAGCGCCTTGTCCACCCAGATTTCTTCAGCCAAAAGTCTCAGACTGAGAAACGGTTCTCGGA
  GAAGCATTCGACCCTGGTGAATGATGCCTACAAGACTCTTCAGGCCCCCGTGAGCAGAGGACTATATCTT
  CTAAAGCTCCAAGGAATAGAAATTCCTGAAGGGACAGATTATAGAACAGACAGTCAGTTCCTTGTGGAAA
  TCATGGAAATCAATGAAAAACTCGCAGACGCCAAAAGTGAGGCAGCCATGGAAGAGGTAGAAGCCACTGT
  CAGAGCTAAACAGAAAGAATTTACGGACAATATAAACAGAGCTTTTGAACAAGGTGATTTTGAAAAAGCC
  AAGGAACTTCTTACAAAAATGAGATACTTTTCAAACATAGAAGAAAAGATCAAGTTAAGCAAGAACCCTC
  TC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206250 representing NM_001108340
  Red=Cloning site Green=Tags(s)
  
  MWSCRARSLLCVWEVRLARFLGRSLLSSNAASGKSIEPQCWNCGRAMGAGRGDEFFCAHCRALQPPDPTR
  DYFSLMNCNQSFRVDIRKLQQRYQQLQRLVHPDFFSQKSQTEKRFSEKHSTLVNDAYKTLQAPVSRGLYL
  LKLQGIEIPEGTDYRTDSQFLVEIMEINEKLADAKSEAAMEEVEATVRAKQKEFTDNINRAFEQGDFEKA
  KELLTKMRYFSNIEEKIKLSKNPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108340
• ORF Size: 702 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108340.1, NP_001101810.1
• RefSeq Size: 783 bp
• RefSeq ORF: 705 bp
• Locus ID: 360826
• Cytogenetics: 12q16
• MW: 27.1 kDa